本科毕业论文
题目： 化学发光免疫分析技术
及在临床检验中的应用
学院： 化学与环境工程学院
班级： 2011级应用化学2班
姓名： 王俊烽
指导教师： 李小花 职称： 讲师
完成日期： 2015 年 05 月 22 日
化学发光免疫分析技术及在临床检验中的应用
摘 要：化学发光免疫分析技术（CLIA）是把免疫反应和化学发光检测方法结合的一种分析技术。其中免疫反应的特异性和灵敏度都很高。CLIA是在酶联免疫分析、放免疫分析和荧光免疫分析后面发展起来的一种检测技术。由于其具有操作简单，标记方便，稳定度和检测灵敏度高，速度快，对环境没有污染等优点因此CLIA在临床上受到医学检验者和医生的一致好评。
关键词：化学发光免疫分析技术；基本原理 ；临床；医学检验
CLIA Technology And Its Application in The Clinical Laboratory
Abstract：Chemiluminescence immunoassay (CLIA) is an analytical technique the immune response and chemiluminescent detection methods combined. Which the immune response is very high specificity and sensitivity. CLIA is the enzyme-linked immunoassay, put immunoassay and fluorescence immunoassay later developed a detection technology. Because of its simple, easy to mark, high stability and sensitivity, speed, no environmental pollution, etc. Therefore CLIA praise medical tests and doctors at the clinic.
Key words :chemiluminescent immunoassay; fundamental; clinical; medical laboratory
目 录
1. CLIA的基本简介 ·················································· 错误！未定义书签。 2. 化学发光免疫分析的原理 ································································· 3
2.1 CLIA原理 ·············································································· 3 2.2器检测原理 ············································································· 3 3 . CLIA的分类 ················································································· 4
3.1发光免疫物质的直接标记分析法 ·················································· 4 3.2化学发光免疫物酶催化分析法 ····················································· 4 3.3发光免疫物的电化学发光分析法(ECUA) ······································· 4 4. 自动化的CLIA分析系统 ································································· 4 5. CLIA技术有关问题探讨 ·································································· 5 6. CLIA在临床检验中的应用 ······························································· 5
6.1可用CLIA来检测分析激素 ························································ 5 6.2 CLIA对类风湿关节炎的检测 ····················································· 6 6.3 CLIA对梅毒的检测 ·································································· 7 6.4 CLIA对艾滋病（AIDS）病毒的检测 ··········································· 7 6.5检测分析肿瘤标志物 ································································· 8 6.6 CLIA对糖尿病的检测 ······························································· 8 6.7 其他物质的检测 ······································································ 8 7. 结 论 ························································································ 9 参考文献 ························································································· 10 致谢 ······························································································· 12