Cell biology experiments report Topic：Sudan Red staining of fat cells in mice Name: Crew: Tutor: Student ID: Time： Digest：
Cells are surrounded by a membrane, independent breeding the smallest group of protoplasm, while the cells are the basic structural and functional units of the activities of life, the cell composition of water, so the cells or organelles under the microscope is a colorless, hard to tell boundaries between cells, in normal circumstances by staining to distinguish cells, staining, usually by some special parts of the cell, or special component into chromatin, and thereby to distinguish from the tissue cells.
In adipose tissue, lipid substances can be stored in the form of oil particles in the fat cells, but in the low magnification under the microscope, the granular material is difficult to see, unless the stain, thus observed. Commonly used to stain the dye Sudan fluid, such as Sudan III, Sudan V.
Sudan Ⅲ is a common right triglycerides were stained fat soluble dye. Its color is reddish brown, the maximum UV absorption of 507 nm, at (304). Sometimes also known as Sudan Red BK, Fat Ponceau G, Cerasin Red, CI 26100, Solvent Red 23, Sudan Red, Sudan Red III, Sudan V, Sudan Red B, Sudan G, Scarlet B, and Tony Red.. In industry, it
is often used to non-polar substances, like oils, fats, waxes, animal fats and oils and other hydrocarbon products, and acrylic emulsion, or emulsion.
Ⅰ, Sudan, Sudan III, Sudan IV has been the International Association for Cancer Research go to the three types of carcinogens, great harm on the human body.
Fat cells to store fat, and adipose tissue by fat cells, which store energy. Mainly by two kinds of adipose tissue, white adipose tissue (WAT) and brown adipose tissue (BAT), which is composed of two different kinds of fat cells in the mouse mesentery is the WAT.
Sudan dyes solubility in lipids is greater than its solubility in solvents, dyes and samples containing lipids in contact, there were a large number of dye into the structure of the lipids, so that these structures were red. And more lipid-soluble dye Sudan Red dye, oil red O, Nile Blue staining, lipid coloring. Can also be used osmium tetroxide staining, fatty acid or choline can osmium tetroxide reduction dioxide osmium black. Mesenteric bilayer membrane of the peritoneum in the posterior wall of the abdomen connected to the jejunum and ileum, that is, it is generally connected to a variety of abdominal part of.
In this experiment, mainly through the mesenteric staining of fat cells in mice, decapitation method directly to the mice killed, separation mice's abdominal cavity at the superior mesenteric, under a microscope after
staining. Background：
Pathological examination, lipid staining, the most commonly used to prove that fatty degeneration, fat emboli, and the identification of the tumor. Lipid staining the most widely used dyes Sudan dyes, the most commonly used with Sudan III, Sudan IV, Sudan black and oil red O. Fat staining, in fact, the Sudan dyes are fat soluble dye adsorption and render color. That the organization of lipids in the liquid or semi-liquid, the coloring effect of the Sudan dyes. According to this theory, an appropriate increase in temperature (37 ° C -60 ° C) Staining effect is good. Materials and Methods： Methods：
1. Cervical dislocation at the expense of mice.
1) Seize the mouse tail, put the mouse on the anatomical plate. Let the front paws of the mice must be on the disk.
2) (Optional) tweezers or scissors gently stroked mice, making it quiet. 3) Tweezers or scissors on the back of the neck of mice, forced down. 4) The tweezers fixed, forced to pull back the mouse tail until the mouse could not move.
5) Forceps fixed fixed period of time, confirm that the mice had died. 2. mice flat on the anatomical plate, abdomen upward. Tweezers to pick up the fur of the lower abdomen, using the scissors cut a small horizontal