LB
鍩瑰吇鍩烘�庢牱閰嶅埗
LB
涓�鑸瑙ｉ噴涓?/p>
Luria-Bertani
锛岀劧鑰屾牴鎹叾鍙戞槑浜鸿礉灏斿灏?/p>
(Giuseppe
Bertani)
鐨勮娉曪紝杩欎釜鍚嶅瓧鏉ユ簮浜庤嫳璇?/p>
lysogeny broth
锛屽嵆婧惰弻鑲夋堡銆?/p>
LB
鍩瑰吇
鍩烘槸杩戝勾鏉ョ敤浜庡煿鍏诲熀鍥犲伐绋嬪彈浣撹弻
(
澶ц偁鏉嗚弻
)
鐨勫父鐢ㄥ煿鍏诲熀涔嬩竴銆?/p>
LB
鍩瑰吇鍩烘槸涓�绉嶅簲鐢ㄦ渶骞挎硾鍜屾渶鏅�氱殑缁嗚弻鍩虹鍩瑰吇鍩猴紝鏈夋椂鍙堢О鏅�氬煿
鍏诲熀锛屽惈鏈夐叺姣嶆彁鍙栫墿銆佽儼鍖栬泲鐧借儴鍜?/p>
NaCl
銆?/p>
閰垫瘝鎻愬彇鐗╋細閰垫瘝缁忕牬澹佸悗灏嗗叾涓泲鐧借川銆佹牳閰搞�佺淮鐢熺礌绛夋娊鎻愶紝鍐嶇粡鐢熺墿
閰惰В鐨勫瘜鍚皬鍒嗗瓙鐨勬皑鍩洪吀銆?/p>
鑲姐�?/p>
鏍歌嫹閰搞�?/p>
缁寸敓绱犵瓑澶╃劧娲绘�ф垚鍒嗙殑娣￠粍鑹茬矇鏈紝
鍏朵腑姘ㄥ熀閰稿惈閲?/p>
30%
浠ヤ笂锛屾�昏泲鐧?/p>
50%
浠ヤ笂锛屾牳鑻烽吀
10%
浠ヤ笂锛屽箍娉涚敤浣滅敓鐗╁煿
鍏诲熀鍜岄鍝佽皟鍛冲搧鍒堕�犲師鏂欍�?/p>
鑳板寲铔嬬櫧鑳細
涓�绉嶄紭璐ㄨ泲鐧借儴锛?/p>
鏄互鏂伴矞鐗涜倝鍜岀墰楠ㄧ粡鑳伴叾娑堝寲锛?/p>
娴撶缉骞茬嚗
鑰屾垚鐨勭櫧鑹茬矇鏈紝鍚湁涓板瘜鐨勬爱婧愩�佹皑鍩洪吀绛夈�?/p>
閰垫瘝鎻愬彇鐗╀负寰敓鐗╂彁渚涚⒊婧愩�佽兘婧愩�佺７閰哥洂銆佺敓闀垮洜瀛愩�佺淮鐢熺礌绛?/p>
;
铔嬬櫧
鑳ㄤ富瑕佹彁渚涙爱婧?/p>
;NaCl
涓昏鎻愪緵寰敓鐗╃敓闀跨幆澧?/p>
(
濡傛笚閫忓帇
)
锛屽叾娆℃槸鎻愪緵鏃犳満
鐩愩�?/p>
鍦ㄩ厤鍒跺浐浣撳煿鍏诲熀鏃惰繕瑕佸姞鍏ヤ竴瀹氶噺鐞艰剛浣滃嚌鍥哄墏銆傜惣鑴傚湪甯哥敤娴撳害涓?/p>
96鈩冩椂婧跺寲锛屼竴鑸疄闄呭簲鐢ㄦ椂鍦ㄦ哺姘存荡涓垨鍨互鐭虫缃戠洿鎺ョ叜娌告憾鍖栥�傜惣鑴傚湪
40鈩冩椂鍑濆浐锛?/p>
閫氬父涓嶈寰敓鐗╁垎瑙ｅ埄鐢ㄣ�?/p>
鍥轰綋鍩瑰吇鍩轰腑鐞艰剛鐨勫惈閲忔牴鎹惣鑴傜殑璐?/p>
閲忓拰姘旀俯鐨勪笉鍚岃�屾湁鎵�涓嶅悓銆?/p>
鐢变簬杩欑鍩瑰吇鍩哄鐢ㄤ簬鍩瑰吇缁嗚弻锛屽洜姝わ紝瑕佺敤绋�閰告垨绋�纰卞皢鍏?/p>
pH
璋冭嚦涓�?/p>
鎴栧井纰辨�э紝浠ュ埄浜庣粏鑿岀殑鐢熼暱绻佹畺銆?/p>
LB
鍩瑰吇鍩洪厤鏂?/p>
(
姣忓崌
)
閰垫瘝鎻愬彇鐗?/p>
5g
鑳板寲铔嬬櫧鑳?/p>
10g
姘寲閽?/p>
10g
NaOH
璋?/p>
pH
鑷?/p>
7.0
锛?21鈩冦�?/p>
20min
楂樺帇鐏弻銆?/p>
LB
鍥轰綋鍩瑰吇鍩?/p>
LB
鍩瑰吇鍩?/p>
1L