楂橀�氶噺娴嬪簭鍦ㄤ复搴婂垎瀛愯瘖鏂腑鐨勫簲鐢ㄤ笌灞曟湜
瀵逛簬鍗曞熀鍥犻仐浼犵梾锛屼互寰�涓村簥瀹為獙瀹や富瑕佸�熷姪浜?/p>
Sanger
娴嬪簭銆佺瓑浣嶅熀鍥犵壒寮傛�ц仛鍚堥叾閾惧弽
搴旓紙
allele
-
specific polymerase chain reaction
锛?/p>
AS
-
PCR
锛?/p>
銆佽崸鍏夊師浣嶆潅浜ゃ�?/p>
DNA
鍗拌鏉備氦绛夋妧鏈?/p>
杩涜妫�楠屻�?/p>
NGS
鎶�鏈拡瀵圭檶鐥囥�佸績琛�绠＄柧鐥呫�佽偩鐥呫�佺硸灏跨梾绛夊鏉傛�х柧鐥呯殑閬椾紶瀛︾瓫鏌ヤ笌璇?/p>
鏂彁渚涗簡渚挎嵎鐨勯�斿緞銆?/p>
鍙﹀锛?/p>
NGS
鎶�鏈湪鐥呭師寰敓鐗╃殑蹇�熼壌瀹氥�?/p>
鑽墿鐨勯澏鍚戞不鐤椾互鍙婁骇鍓?/p>
绛涙煡绛夊涓鍩熷叿鏈夋綔鍦ㄧ殑搴旂敤浼樺娍銆?/p>
1
娴嬪簭鎶�鏈殑鍙戝睍鍙婃�ц兘姣旇緝
2006
骞达紝
Illumina
鍏徃鎺ㄥ嚭浜?/p>
Solexa
娴嬪簭骞冲彴銆?/p>
鐩墠锛岃鍏徃宸茬粡鎺ㄥ嚭浜嗗绉嶅瀷鍙风殑娴嬪簭骞?/p>
鍙帮紝濡?/p>
MiSeq
銆?/p>
HiSeq
銆?/p>
NextSeq
绛夌郴鍒楋紝鍏朵腑
MiSeq
绯诲垪閫傚悎浜庡皬鍨嬪熀鍥犵粍娴嬪簭锛?/p>
HiSeq
绯?/p>
鍒楅�傜敤浜庡ぇ鍨嬪熀鍥犵粍娴嬪簭銆?/p>
2007
骞达紝缇庡浗搴旂敤鐢熺墿绯荤粺鍏徃鎺ㄥ嚭
SOLiD
娴嬪簭骞冲彴銆傝骞冲彴
閲囩敤浜旇疆娴嬪簭娉曚互
4
鑹茶崸鍏夋爣璁板鏍歌嫹閰哥殑杩炴帴鍚堟垚涓哄熀纭�锛屾祴搴忓噯纭�у緱浠ユ彁楂樸�?/p>
2010
骞达紝
缇庡浗鐢熷懡绉戝鍏徃鍜屽お骞虫磱鐢熺墿绉戝鍏徃鍒嗗埆鍙戝竷浜嗗崐瀵间綋娴嬪簭骞冲彴鍜岀
3
浠ｅ崟鍒嗗瓙瀹?/p>
鏃讹紙
single molecule realtime
锛?/p>
SMRT
锛?/p>
DNA
娴嬪簭骞冲彴銆傝繖
2
绉嶆祴搴忔妧鏈笌浠ュ線鐨勫熀浜庡厜瀛︿俊
鍙风殑妫�娴嬫妧鏈笉鍚岋紝
鍗婂浣撴祴搴忓钩鍙伴�氳繃鍗婂浣撹姱鐗囩洿鎺ユ劅搴斿湪搴忓垪鍚堟垚杩囩▼涓７閰镐簩閰敭
3'OH
鍩哄洟閲婃斁鐨勮川瀛愶紱绗?/p>
3
浠ｆ祴搴忎华閫氳繃绾崇背瀛旀妧鏈褰曞崟涓仛鍚堥叾鍦ㄤ笉鍙楀共鎵版儏鍐典笅杩?/p>
缁悎鎴愶紝
鍏朵腑
PacBio RS II
姣忔杩愯鑳藉浜х敓
60 000
脳
16
鏉″簭鍒楋紝
姣忔潯搴忓垪鐨勫钩鍧囬暱搴﹁揪
8 500
bp
銆?/p>
涓�鑸潵璇达紝
浠ヤ笂姣忕娴嬪簭浠湪搴忓垪璇绘闀垮害銆?/p>
鍑嗙‘鎬с�佹祴搴忛�氶噺銆?/p>
浠锋牸绛夊涓柟闈㈠瓨鍦ㄤ竴瀹?/p>
鐨勫樊寮傘�傜劍纾烽吀娴嬪簭骞冲彴娴嬪簭璇绘杈冮暱锛屾祴搴忛�氶噺杈冧綆锛屾垚鏈浉瀵硅緝楂橈紱
Illumina
绯诲垪骞冲彴
浜х敓鐨勮娈电浉瀵硅緝鐭紝娴嬪簭璐圭敤鐩稿杈冧綆锛屽簲鐢ㄦ瘮杈冨箍娉涳紱
SOLiD
娴嬪簭骞冲彴鍦ㄩ�氶噺鍜屽噯纭�?/p>
鏂归潰鐩稿浠ヤ笂
2
绉嶇被鍨嬬殑娴嬪簭骞冲彴鏈夋槑鏄炬敼鍠勶紝浣嗘槸娴嬪簭闀垮害鏇寸煭锛涘崐瀵间綋娴嬪簭骞冲彴浠ュ強
SMRT
娴嬪簭骞冲彴鐩告瘮鍏朵粬娴嬪簭骞冲彴杩愯鏃堕棿杈冪煭锛屽彟澶栧崟鍒嗗瓙娴嬪簭骞冲彴鍑忓皯浜嗘祴搴忓墠鐨勬墿澧?/p>
鍑嗗宸ヤ綔锛屾祴搴忚娈佃緝闀匡紝浣嗘槸娴嬪簭鎴愭湰鍜岄敊璇巼閮界浉瀵硅緝楂?/p>
[8
-
10]
銆備竴浜涘父鐢ㄧ殑娴嬪簭浠殑
娴嬪簭鍘熺悊鍜屾�ц兘瑙佽〃
1
銆?/p>
