甯哥敤澶ц偁鏉嗚弻鎰熷彈鎬?/p>
JM109
锛?/p>
DH5a
锛?/p>
BL21
鐨勫尯鍒?/p>
1
锛?/p>
DH5a
鑿屾牚
DH5a
鏄竴绉嶅父鐢ㄤ簬璐ㄧ矑鍏嬮殕鐨勮弻鏍�?/p>
E.coli DH5a
鍦ㄤ娇鐢?/p>
pUC
绯诲垪璐ㄧ矑杞戒綋杞?/p>
鍖栨椂锛屽彲涓庤浇浣撶紪鐮佺殑
尾
锛嶅崐涔崇硸鑻烽叾姘ㄥ熀绔疄鐜?/p>
伪
锛嶄簰琛ャ�傚彲鐢ㄤ簬钃濈櫧鏂戠瓫閫?/p>
閴村埆閲嶇粍鑿屾牚銆?/p>
鍩哄洜鍨嬶細
F-
锛?/p>
蠁80dlacZ螖M15
锛?/p>
螖(lacZYA
-argF)U169
锛?/p>
deoR
锛?/p>
recA1
锛?/p>
endA1
锛?/p>
hsdR17(rk-
锛?/p>
mk+)
锛?/p>
phoA
锛?/p>
supE44
锛?/p>
位
-
锛?/p>
thi-1
锛?/p>
gyrA96
锛?/p>
relA1
2
锛?/p>
BL21(DE3)
鑿屾牚
璇ヨ弻鏍敤浜庨珮鏁堣〃杈惧厠闅嗕簬鍚湁鍣弻浣?/p>
T7
鍚姩瀛愮殑琛ㄨ揪杞戒綋锛堝
pET
绯诲垪锛?/p>
鐨勫熀鍥犮�?/p>
T7
鍣弻浣?/p>
RNA
鑱氬悎閰朵綅浜?/p>
位
鍣弻浣?/p>
DE3
鍖猴紝璇ュ尯鏁村悎浜?/p>
BL21
鐨勬煋
鑹蹭綋涓娿�傝鑿岄�傚悎琛ㄨ揪闈炴瘨鎬ц泲鐧姐�?/p>
鍩哄洜鍨嬶細
F-
锛?/p>
ompT
锛?/p>
hsdS
锛?/p>
rBB-mB
锛嶏級锛?/p>
gal
锛?/p>
dcm
锛?/p>
DE3
锛?/p>
3
锛?/p>
BL21(DE3) pLysS
鑿屾牚
璇ヨ弻鏍惈鏈夎川绮?/p>
pLysS
锛屽洜姝ゅ叿鏈夋隘闇夌礌鎶楁�с�?/p>
PLysS
鍚湁琛ㄨ揪
T7
婧惰弻閰剁殑鍩?/p>
鍥狅紝鑳藉闄嶄綆鐩殑鍩哄洜鐨勮儗鏅〃杈炬按骞筹紝浣嗕笉骞叉壈鐩殑铔嬬櫧鐨勮〃杈俱�傝鑿岄�傚悎
琛ㄨ揪姣掓�ц泲鐧藉拰闈炴瘨鎬ц泲鐧姐�?/p>
鍩哄洜鍨嬶細
F-
锛?/p>
ompT hsdS
锛?/p>
rBB-mB
锛嶏級锛?/p>
gal
锛?/p>
dcm
锛?/p>
DE3
锛?/p>
pLysS
锛?/p>
Camr
4
锛?/p>
JM109
鑿屾牚
璇ヨ弻鏍湪浣跨敤
pUC
绯诲垪璐ㄧ矑杞戒綋杩涜
DNA
杞寲鎴栫敤
M13 phage
杞戒綋杩涜杞煋
鏃讹紝鐢变簬杞戒綋
DNA
浜х敓鐨?/p>
LacZa
澶氳偨鍜?/p>
JM09
缂栫爜鐨?/p>
LacZ螖M15
杩涜
伪
锛嶄簰
琛ワ紝浠庤�屾樉绀?/p>
尾
锛嶅崐涔崇硸鑻烽叾娲绘�э紝鐢辨寰堝鏄撻壌鍒噸缁勪綋鑿屾牚
鍩哄洜鍨嬶細
recA1
锛?/p>
endA1
锛?/p>
gyrA96
锛?/p>
thi
锛?/p>
1
锛?/p>
hsdR17
锛?/p>
supE44
锛?/p>
relA1
锛?/p>
螖
锛?/p>
lac
锛?/p>
proAB
锛?/p>
/F鈥橻traD36
锛?/p>
proAB+
锛?/p>
lacIq
锛?/p>
lacZ螖M15]
5
锛?/p>
TOP10
鑿屾牚
璇ヨ弻鏍�傜敤浜庨珮鏁堢殑
DNA
鍏嬮殕鍜岃川绮掓墿澧烇紝鑳戒繚璇侀珮鎷疯礉璐ㄧ矑鐨勭ǔ瀹氶仐浼犮�?/p>
鍩哄洜鍨嬶細
F-
锛?/p>
mcrA螖(mrr
-hsd RMS-mcrBC)
锛?/p>
蠁80
锛?/p>
lacZ螖M15
锛?/p>
鈻?/p>
lac
鈪?/p>
74
锛?/p>
recA1
锛?/p>
ara螖139螖(ara
-leu)7697
锛?/p>
galU
锛?/p>
galK
锛?/p>
rps
锛?/p>
(Strr) endA1
锛?/p>
nupG
6
锛?/p>
HB101
鑿屾牚
璇ヨ弻鏍仐浼犳�ц兘绋冲畾锛屼娇鐢ㄦ柟渚匡紝閫傜敤浜庡悇绉嶅熀鍥犻噸缁勫疄楠?/p>
鍩哄洜鍨嬶細
supE44
锛?/p>
hsdS20
锛?/p>
rB-mB
锛嶏級锛?/p>
recA13
锛?/p>
ara
锛?/p>
14
锛?/p>
proA2
锛?/p>
lacY1
锛?/p>
galK2
锛?/p>
rpsL20
锛?/p>
xyl-5
锛?/p>
mtl-1
锛?/p>
leuB6
锛?/p>
thi-1
7
锛?/p>
M110
鎴?/p>
SCS110
澶у鏁板ぇ鑲犳潌鑿岃弻鏍腑鍚湁
Dam
鐢插熀鍖栭叾鍜?/p>
Dcm
鐢插熀鍖栭叾锛屽墠鑰呭彲浠ュ湪
GATC
搴忓垪涓吅鍢屽懁
N-6
浣嶄笂寮曞叆鐢插熀锛屽悗鑰呭湪
CCA/TGC
搴忓垪鐨勭涓�涓優鍢?/p>
鍟?/p>
C-5
浣嶇疆涓婂紩鍏ョ敳鍩恒�?/p>
甯哥敤鐨勮弻鏍兘浼氫骇鐢?/p>
dam,dcm
锛屼粠鑰屽彈鍒扮敳鍩哄寲鐨勫奖