Ensembl
data bank
鐢插熀鍖栨祴搴?/p>
BSP
娉?/p>
鐨勯偅涓粦鐧界偣鐘跺浘
锛堥粦琛ㄧず鐢插熀鍖栥�?/p>
鐧界偣琛ㄧず闈炵敳鍩哄寲锛?/p>
鏄?/p>
鎬庝箞鍋氬嚭鏉ョ殑鍛�
涓?/p>
鐢?/p>
BiQ ANALYZER
杞欢锛屽厤璐圭殑鍙互涓嬭浇锛屽畨瑁呴渶瑕佹渶鏂扮殑
java
绋嬪簭
锛屽叧浜庡悗缁殑涓�浜涙楠わ紝鎴戠湅浜嗕竴绡囧浗鍐呯殑纭曞＋璁烘枃锛屽涓嬶細
2.2.1.4.6
杩炴帴鍙嶅簲浜х墿鐨勮浆鍖?/p>
(l)
姣忎釜杩炴帴鍙嶅簲鍑嗗
1
涓惈鏈夋皑鑺傞潚闇夌礌鐨?/p>
LB
骞虫澘锛屾秱鏉垮墠鍗婂皬鏃跺皢骞虫澘浠庡啺绠?/p>
涓彇鍑哄钩琛¤嚦瀹ゆ俯銆?/p>
(2)
绂诲績浣胯繛鎺ュ弽搴斿唴瀹圭墿姹囬泦鍒扮搴曪紝鍚稿彇
10ul
杩炴帴鍙嶅簲浜х墿鍔犲埌缃簬鍐颁笂鐨?/p>
1.5ml
绂诲績绠′腑
.
(3)
灏嗗喕瀛樼殑
JM109
楂樻晥鐜囨劅鍙楁�佺粏鑳炰粠涓�
70
鈩冨啺绠变腑鍙栧嚭锛屾斁缃湪鍐版荡鐩磋嚦铻嶅寲
(
澶ф
5
鍒嗛挓
)
锛岃交杞绘尟鍔ㄧ蹇冪浣夸箣娣峰寑銆?/p>
(4)
鍚戞楠?/p>
2
鍑嗗鐨勬瘡涓浆鍖栫涓姞鍏?/p>
50ul
鎰熷彈鎬佺粏鑳炪�?/p>
(5)
杞昏交鎸姩灏忕娣峰寑锛屽啺娴?/p>
30
鍒嗛挓銆?/p>
(6)
鍦ㄧ簿纭殑
42
鈩冩按娴翠腑鐑嚮
45
涓�
50
绉?/p>
(
涓嶈鎸姩
)
銆?/p>
(7)
杩呴�熷皢绠″瓙绉诲埌鍐版荡涓紝浣跨粏鑳炲喎鍗?/p>
2
鍒嗛挓銆?/p>
(8)
姣忕杩炴帴鍙嶅簲杞寲缁嗚優涓姞鍏ュ钩琛¤嚦瀹ゆ俯鐨?/p>
200ulLB
鍩瑰吇鍩猴紝
(9)
鍦?/p>
37
鈩冩尟鑽″煿鍏?/p>
(150rpm)1
灏忔椂銆?/p>
(10)
灏嗘瘡涓浆鍖栧煿鍏诲熀
200ul
娑傚埌
LB/
姘ㄨ媱
/IPTG/X-Gal
骞虫澘涓娿�?/p>
(11)
灏嗗钩鏉夸簬
37
鈩冩亽娓╃涓繃澶滃煿鍏?/p>
(16
涓�
24
灏忔椂
)
銆?/p>
2.2.L4.7
闃虫�у厠闅嗙瓫閫?/p>
浠庡煿鍏荤涓彇鍑哄钩鏉匡紝缃簬
4
鈩冨啺绠变娇钃濊壊鍏呭垎鏄剧幇銆傛寫鍙栫櫧鏂戣弻钀藉埌
5mLB
鍩?/p>
鍩猴紝
37
鈩冩憞搴婁笂鍩瑰吇杩囧銆傚惛鍙?/p>
lml
鑿屾恫閫佹祴搴忥紝寮曠墿涓洪�氱敤寮曠墿
M13
銆?/p>
杩涘叆涓侀鍥紝
鏄粠鍋?/p>
MSP
寮�濮嬬殑銆?/p>
浠庡
DNA
鐢插熀鍖栫殑涓�鏃犳墍鐭ュ埌
MSP
瀹為獙鎴愬姛锛?/p>
缁忓巻浜?/p>
寰堝鑹伴毦锛屽悓鏃朵篃鏀惰幏寰椾簡涓嶅皯缁忛獙銆備粠涓侀鍥殑甯栧瓙鍙互鐪嬪嚭锛岃繎鍑犲勾鏉ワ紝鍥藉唴瀵?/p>
DNA
鐢插熀鍖栫殑鐮旂┒鍦ㄩ�愬勾澧炲姞锛屾垬鍙嬩滑鍦ㄥ疄楠屼腑閬囧埌鐨勫洶闅句篃涓嶅皯銆傚湪杩欓噷璋堜竴涓嬪
MSP
,
鍙?/p>
BSP
鐨勪竴鐐逛綋浼氾紝甯屾湜鑳藉鏂版墜浠湁鎵�甯姪锛屼篃璇疯�佹墜浠壒璇勮ˉ鍏呫�?/p>
浜氱～閰告阿鐩愯浆鍖栧拰
PCR
鎵╁鏄?/p>
MSP
,BSP
鐨勪袱涓熀鏈楠ゃ�備簹纭吀姘㈢洂杞寲锛屼及璁＄幇鍦ㄥ仛
鎵嬪伐淇グ鐨勪篃涓嶅浜嗭紝璇曞墏鐩掕兘澶熷緢鏂逛究鐨勫府鎴戜滑瑙ｅ喅闂銆備絾
PCR
涓殑寮曠墿璁捐锛岀幇鍦?/p>
鍙兘杩樻槸涓毦棰樸�傜湅鍒板緢澶氬笘瀛愰噷鎻愬埌鐨勫紩鐗╅兘鏄潵婧愪簬鏂囩尞鐨勶紝浣嗕簨瀹炰笂鍦ㄥ緢澶氭儏鍐典笅锛?/p>
鎴戜滑鏄病娉曟壘鍒扮幇瀛樺紩鐗╃殑锛屽洜姝ゆ帉鎻?/p>
BSP
,MSP
寮曠墿鐨勮璁″氨鏄惧緱闈炲父蹇呰浜嗐�?/p>
BSP
锛?/p>
MSP
寮曠墿璁捐鐨勪竴涓渶澶ч棶棰樺湪浜庝簹纭吀姘㈢洂鐨勮浆鍖栦娇寰楁ā鏉跨殑搴忓垪澶嶆潅鎬уぇ澶ч檷
浣庯紝
鑰屼笖缁忓父浜х敓
T
鎴?/p>
G
鎴栬�呭瘜鍚?/p>
GC
鐗囨浜ゆ浛鍑虹幇鐨勫簭鍒椼�?/p>
杩欎釜浼氫娇寮曠墿閫夋嫨鍙樺緱鍥伴毦銆?/p>
鍥犳锛屽線寰�鎴戜滑闇�瑕佽璁″发寮忓紩鐗╂潵瑙ｅ喅杩欎釜闂銆?/p>
BSP
,MSP
寮曠墿鐨勮璁★紝闄や簡瑕侀伒寰紩
鐗╄璁＄殑鍩烘湰鍘熷垯澶栵紝杩樿娉ㄦ剰涓�涓嬩竴浜涜鍒欙細