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1
璇鹃
2
澶氳仛閰堕摼寮忓弽搴旀墿澧?/p>
DNA
鐗囨
涓�銆佸崟椤归�夋嫨棰?/p>
1
锛庝笅鍒楁湁鍏?/p>
PCR
鐨勬弿杩帮紝涓嶆纭殑鏄?/p>
(
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A
锛?/p>
PCR
鎶�鏈殑鍘熺悊鏄?/p>
DNA
澶嶅埗
B
锛庣敤
PCR
鎶�鏈墿澧?/p>
DNA
鏄竴涓叾淇冨弽搴旓紝闇�鑰愰珮娓╃殑瑙ｆ棆閰跺拰
DNA
鑱氬悎閰?/p>
C
锛庝竴涓?/p>
DNA
鐗囨缁?/p>
PCR
鎵╁锛屽彲褰㈡垚
2
n
n
瑙ｆ瀽锛?/p>
PCR
鏄竴绉嶄綋澶栬繀閫熸墿澧?/p>
DNA
鐗囨鐨勬妧鏈紝瀹冧互鏋佸皯閲忕殑
DNA
涓烘ā鏉匡紝浠ュ洓绉嶈劚
姘ф牳鑻烽吀涓哄師鏂欙紝鍦ㄥ紩鐗╀綔鐢ㄤ笅浣?/p>
DNA
鑱氬悎閰朵粠寮曠墿鐨?/p>
3'
绔繛鎺ヨ劚姘ф牳鑻烽吀锛岀煭鏃堕棿鍐呰繀
閫熷鍒朵笂鐧句竾浠界殑
DNA
鎷疯礉銆?/p>
PCR
鍒╃敤
DNA
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鍚堝紩鐗╋紝涓嶇敤瑙ｆ棆閰惰В鏃嬨�?/p>
绛旀锛?/p>
B
2.PCR
鎶�鏈渶绐佸嚭鐨勪紭鐐规槸
(
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A
锛庡師鐞嗙畝鍗?/p>
B
锛庡師鏂欐槗鎵?/p>
C
锛?/p>
Taq
DNA
鑱氬悎閰舵湁鑰愮儹鎬?/p>
D
锛庡揩閫熴�侀珮鏁堛�佺伒娲汇�佹槗浜庢搷浣?/p>
绛旀锛?/p>
D
3
锛?/p>
PCR
鎶�鏈殑鎿嶄綔姝ラ渚濇鏄?/p>
(
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A
锛庨珮娓╁彉鎬с�佷腑娓╁欢浼搞�佷綆娓╁鎬?/p>
B
锛庨珮娓╁彉鎬с�佷綆娓╁鎬с�佷腑娓╁欢浼?/p>