瀹炵敤姹囨�绘姤鍛?/p>
1 / 6
瀹為獙鍘熺悊
铔嬬櫧璐ㄥ嵃杩规槸鎶婄數娉冲垎绂荤殑铔嬬櫧璐ㄨ浆绉诲埌鍥哄畾鍩鸿川涓婏紝鐒跺悗鍒╃敤鎶楀師鎶椾綋鍙嶅簲鏉ユ娴嬬壒寮?/p>
鎬х殑铔嬬櫧鍒嗗瓙鐨勬妧鏈紝鍖呮嫭涓変釜閮ㄥ垎锛?/p>
SDS
鈥旇仛涓欑儻閰拌兒鍑濊兌鐢垫吵锛岃泲鐧借川鐨勭數娉宠浆绉伙紝鍏嶇柅
鍗拌抗鍒嗘瀽銆?/p>
SDS
鈥旇仛涓欑儻閰拌兒鍑濊兌鐢垫吵涓昏鐢ㄤ簬娴嬪畾铔嬬櫧璐ㄧ浉瀵瑰垎瀛愯川閲忥紝
SDS
鏄槾绂诲瓙鍘绘薄鍓傦紝鑳芥柇
瑁傝泲鐧借川鍒嗗瓙鍐呭拰鍒嗗瓙闂寸殑姘㈤敭锛屼娇鍒嗗瓙鍘绘姌鍙狅紝鐮村潖鍏堕珮绾х粨鏋勩�?/p>
SDS
涓庡ぇ澶氭暟铔嬬櫧璐ㄧ殑缁?/p>
鍚堟瘮涓?/p>
1.4:1
锛岀敱浜?/p>
SDS
甯︽湁澶ч噺鐨勮礋鐢佃嵎锛屼笌铔嬬櫧璐ㄧ粨鍚堟椂鎺╃洊浜嗕笉鍚岀绫昏泲鐧借川闂村師鏈夌殑
鐢佃嵎宸埆锛屼娇鍚勭铔嬬櫧璐ㄥ甫鏈夌浉鍚屽瘑搴︾殑璐熺數鑽凤紝褰技闀挎き鍦嗘锛岃泲鐧借縼绉荤巼涓庤泲鐧借川鐩稿鍒?/p>
瀛愯川閲忕殑瀵规暟鍛堢嚎鎬у叧绯汇�傚洜姝わ紝鍒╃敤鐩稿鍒嗗瓙璐ㄩ噺鏍囧噯铔嬬櫧鎵�浣滅殑鏍囧噯鏇茬嚎锛屽彲浠ユ眰寰楁湭鐭?/p>
铔嬬櫧鐨勭浉瀵瑰垎瀛愯川閲忋�?/p>
鐢垫吵鍚庤泲鐧借川鍒嗗瓙宓屽湪鍑濊兌浠嬭川涓紝鎺㈤拡鍒嗗瓙寰堥毦閫氳繃鍑濊兌瀛旓紝灏嗚泲鐧借川浠庡嚌鑳惰浆绉诲埌鍥?/p>
瀹氬熀璐ㄤ笂鍙互瀵硅泲鐧借川杩涜鍏嶇柅妫�娴嬪垎鏋愩�傛柟娉曟湁涓ょ锛氣憼姘村钩鍗婂共寮忚浆绉诲嵆灏嗗嚌鑳跺拰鍥哄畾鍩?/p>
璐ㄤ技涓夋槑娌绘牱澶瑰湪缂撳啿娑叉蹈婀跨殑婊ょ焊涓棿锛岄�氱數
10~30min
鍙畬鎴愨憽鍨傜洿婀垮紡杞Щ鍗冲皢鍑濊兌鍜?/p>
鍥哄畾鍩鸿川澶瑰湪婊ょ焊涓棿锛屾蹈鍦ㄨ浆绉昏缃殑缂撳啿娑蹭腑锛岄�氱數
2~4h
鎴栬繃澶滃彲瀹屾垚銆傚浐瀹氬熀璐ㄩ�氬父
鏈夌閰哥氦缁寸礌鑶溿�佽仛鍋忎簩姘熶箼鐑啘鍜屽凹榫欒啘銆?/p>
铔嬬櫧璐ㄨ浆绉诲埌鍥哄畾鍖栬啘涓婁箣鍚庯紝閫氳繃铔嬬櫧璐ㄦ煋鏂欏涓芥槬绾?/p>
S
妫�娴嬭啘涓婄殑鎬昏泲鐧斤紝鎴栫敤鑰冮┈
鏂寒钃濇娴嬪嚌鑳朵笂鐨勮泲鐧藉墿浣欓噺锛屼互楠岃瘉杞Щ鏄惁鎴愬姛銆傜敤鎶椾綋浣滀负鎺㈤拡杩涜鐗瑰紓鎬х殑鍏嶇柅鍙?/p>
搴旀娴嬫姉鍘熻泲鐧斤紝鍒嗕负
4
姝ワ細鈶犵敤闈炵壒寮傛�с�侀潪鍙嶅簲娲绘�у垎瀛愬皝闃诲浐瀹氬寲鑶滀笂鏈惛闄勮泲鐧界殑鑷?/p>
鐢辩粨鍚堝尯锛屼互闃叉浣滀负鎺㈤拡鐨勬姉浣撶粨鍚堝埌鑶滀笂锛屽嚭鐜版娴嬫椂鐨勯珮鑳屾櫙鈶″浐瀹氬寲鑶滅敤涓撲竴鎬х殑涓�
鎶楁俯鑲诧紝浣夸竴鎶椾笌鑶滀笂鐨勬姉鍘熻泲鐧藉垎瀛愮壒寮傛�х粨鍚堚憿閰舵爣浜屾姉涓庝竴鎶楃壒寮傜粨鍚堚懀鍔犲叆閰跺簳鐗╋紝
閫傚綋淇濇俯锛岃啘涓婁究鍙鍒伴鑹插弽搴旓紝妫�娴嬪嚭鎶楀師铔嬬櫧鍖哄甫銆?/p>
涓昏婧舵恫
10%
鍒嗙鑳?/p>
姘?/p>
3.3mL
銆?/p>
30%
涓?/p>
鐑?/p>
閰?/p>
鑳?/p>
娣?/p>
鍚?/p>
娑?/p>
4.0mL
銆?/p>
1.0mol/L
Tris
锛?/p>
pH8.8
锛?/p>
2.5mL
銆?/p>
10%
SDS
0.1mL
銆?/p>
10%
杩囩～閰搁摰
0.1mL
銆?/p>
TEMED 0.004mL
5%
娴撶缉鑳?/p>
姘?/p>
2.7mL
銆?/p>
30%
涓欑儻閰拌兒娣峰悎娑?/p>
0.67mL
銆?/p>
1.0mol/LTris0.5mL
銆?/p>
10%SDS0.04Ml/10%
杩囩～閰搁摰
0.04mL
銆?/p>
TEMED0.004mL
1
脳
Tris
鈥撶敇姘ㄩ吀鐢垫吵缂撳啿娑?/p>
Tris
纰?/p>
3.03g
銆佺敇姘ㄩ吀
18.77g
銆?/p>
SDS 1g
锛岀敤鍘荤瀛愭按瀹氬鑷?/p>
1L
2
脳
SDS
鍑濊兌鍔犳牱缂撳啿娑?/p>
Tris-HCl
锛?/p>
pH6.8
锛?/p>
100mmol/L
锛屛?/p>
-
宸熀涔欓唶
10%
锛?/p>
10%
鐢樻补锛?/p>
0.01%
婧撮厷钃濓紝
10%SDS
杞Щ缂撳啿娑?/p>
Tris 2.45g
锛岀敇姘ㄩ吀
11.25g
锛岀敳閱?/p>
100mL
锛屽姞鍘荤瀛愭按鑷?/p>
1L
TBST
Tris 1.21g NaCl 8.77g
锛?/p>
Tween-20 1mL
锛屽姞鍘荤瀛愭按鑷?/p>
1L
Stripping
1.3mL Tris
锛?/p>
pH 6.8
锛夛紝
4mL10%SDS
锛?40碌l尾
-
宸熀涔欓唶锛岀敤姘村畾瀹瑰埌
20mL
瀹為獙姝ラ
1 SDS
鈥旇仛涓欑儻閰拌兒鍑濊兌鐢垫吵
鈶村嚌鑳堕厤缃?/p>
鈶犲垎绂昏兌鐨勯厤缃細灏嗛厤缃ソ鐨勫垎绂昏兌娑叉贩鍖�鍚庤繀閫熷�掑叆鑳舵Ы涓紝鑷宠窛绂荤煭鐜荤拑鏉块《绔害
2cm
澶勶紝鍋滄鐏岃兌銆傛鏌ユ槸鍚︽湁姘旀场锛岃嫢鏈夌敤婊ょ焊鏉″惛鍑恒�傜劧鍚庡湪鑳舵恫鐣岄潰涓婂姞钂搁姘磋繘琛屾按灏併�?/p>
15~30min
鍚庯紝鍑濊兌鍜屾按灏佸眰鐣岄潰娓呮櫚锛岃鏄庤兌宸茬粡鑱氬悎瀹屽叏锛岀劧鍚庣敤婊ょ焊鍚稿彇姘村皝灞傦紝婊ょ焊鍒?/p>
鍕挎帴瑙﹀埌鍑濊兌闈€�傦紙浣胯泲鐧芥牱鍝佸垎绂伙級
鈶℃祿缂╄兌鐨勯厤缃細灏嗛厤缃ソ鐨勬祿缂╄兌鐏屾敞鍦ㄥ垎绂昏兌涔嬩笂锛岀洿鑷崇煭鐜荤拑鏉跨殑椤剁锛岀劧鍚庢彃鍏ユ牱鍝?