鐢熺墿鍖栧璇曞嵎锛?/p>
2
宸伙級
涓�銆佸悕璇嶈В閲?/p>
(
姣忎釜
2
鍒嗭紝鍏?/p>
10
鍒?/p>
)
1.
閰剁殑杞崲鏁?/p>
2.
铔嬬櫧鑱氱硸
3.
鎽嗗姩閰嶅
4. Southern
鏉備氦
5.
缁撴瀯鍩?/p>
浜屻�佸～
绌?/p>
(
姣忕┖
0.5
鍒嗭紝鍏?/p>
10
鍒?/p>
)
1
锛庣淮鐢熺礌
B
1
鏋勬垚鐨勮緟閰舵槸
TPP
锛屽鏋滅己涔忥紝浼氶�犳垚
鍜屼钩閰稿湪绁炵粡缁勭粐涓爢绉�?/p>
2
锛庢皑鍩鸿澏鍛よ兘涓?/p>
鍙戠敓涓嶅彲閫嗙粨鍚堬紝闃绘鍥涙阿鍙堕吀鐨勭敓鎴愶紝鎶戝埗浜?/p>
FH
4
鍙備笌鐨勫悇
绉?/p>
杞Щ鍙嶅簲銆?/p>
3
锛庨珮娴撳害鐨?/p>
浼氶檷浣?/p>
6-
纾烽吀鏋滅硸婵�閰跺
6-
纾烽吀鏋滅硸鐨勪翰鍜屽姏锛屾閰舵渶寮虹殑鍒瀯婵�娲?/p>
鍓傛槸
銆?/p>
4
锛庝互涔欓叞
CoA
涓哄師鏂欏彲鍚堟垚鐨勫寲鍚堢墿鏈?/p>
銆?/p>
銆?/p>
绛夈�?/p>
5
锛?/p>
DNA
澶嶅埗鏃朵笌
DNA
瑙ｉ摼鏈夊叧鐨勯叾鍜岃泲鐧借川鏈?/p>
銆?/p>
鍜?/p>
銆?/p>
6
锛?/p>
涔欓叞杈呴叾
A
杩涘叆涓夌晶閰稿惊鐜紝杩樻槸褰㈡垚閰綋
,
鐢辩粏鑳炰腑
鐨勬祿搴﹀喅瀹?/p>
7
锛庡悎鎴愯姵棣欐棌姘ㄥ熀閰哥浉鍚岀殑涓�娈佃繃绋嬬О涓?/p>
銆?/p>
8
锛?/p>
鍔熻兘澶嶆潅鎴栧鏍风殑鑶滐紝
鍗犵殑姣斾緥杈冨ぇ锛?/p>
鑶滀腑
鐨勫瓨鍦ㄥ彲鑳藉鐢熺墿鑶滀腑鑴傜被鐨勭墿
鐞嗙姸鎬佹湁涓�瀹氳皟鑺備綔鐢ㄣ�?/p>
9
锛?/p>
缇庡浗绉戝瀹?/p>
P.Agre
鍥犲彂鐜扮粏鑳炶啘涓婄殑姘村垎瀛愰�氶亾鑰岃幏
骞村害璇鸿礉灏斿寲瀛﹀鐨勪竴
鍗娿�?/p>
鍙︿竴浣嶇編鍥界瀛﹀
MacKinnon
鍥犲缁嗚優鑶滀笂绂诲瓙閫氶亾缁撴瀯鍜屼綔鐢ㄦ満鐞嗙殑鐮旂┒鑰?/p>
鑾锋濂栭」鐨勫彟涓�鍗娿�?/p>
涓夈�佸垽鏂锛堟纭啓
T
锛岄敊璇啓
F
锛屾瘡棰?/p>
1
鍒嗭紝鍏?/p>
8
鍒嗭級
1
锛?/p>
鐢?/p>
PITC
澶勭悊涓�灏忚偨锛?/p>
锛屾湭娴嬪嚭娓哥鐨?/p>
伪
-NH
2
锛屽垯姝よ偨蹇呬负鐜偨銆?/p>
2
锛?/p>
褰撴煇涓�姘ㄥ熀閰告櫠浣撴憾浜?/p>
pH7.0
鐨勬按涓悗锛屾墍寰楁憾娑茬殑
pH
涓?/p>
8.0
锛屽垯姝ゆ皑鍩洪吀鐨?/p>
pI
鐐瑰繀澶т簬
8.0
銆?/p>
3
锛?/p>
鍦ㄤ竴瀹氭哀鍖栧帇鏉′欢涓嬶紝琛�绾㈣泲鐧戒笌姘х殑浜插拰鍔涜緝鑲岀孩铔嬬櫧鏇村己銆?/p>
4
锛?/p>
Sanger
鎻愬嚭鐨?/p>
DNA
鏍歌嫹閰搁『搴忔祴瀹氭硶鏄弻鑴辨哀鏈缁堟娉曘�?/p>
5
锛?/p>
鑹叉皑閰告搷绾靛瓙涓瓨鍦ㄨ“鍑忓瓙锛屾晠姝ゆ搷绾电郴缁熸湁缁嗚皟鑺傚姛鑳姐�?/p>
6
锛庡皢姘ㄥ熀閰镐唬璋㈠拰鏍歌嫹閰镐唬璋㈣仈绯昏捣鏉ョ殑鏋㈢航鍖栧悎鐗╂槸
SAM
鍜?/p>
FH
4
7
锛?/p>
DIFP
鑳藉涓庨叾娲绘�х洿鎺ョ浉鍏崇殑
Cys
涓婄殑鐤忓熀缁撳悎锛屼粠鑰屾姂鍒舵煇浜涢叾銆?/p>
8
锛庨楠艰倢銆佸績鑲屻�佽倽鑴忎互鍙婅剳缁勭粐鐨勮劚姘ㄥ熀鏂瑰紡鏄互鍢屽懁鏍歌嫹閰稿惊鐜负涓汇�?/p>
鍥涖�侀�夋嫨棰橈紙姣忛
1
鍒嗭紝鍏?/p>
8
鍒嗭級
1
锛庤泲鐧借川涓夌淮缁撴瀯鐨勬瀯璞＄壒寰佷富瑕佸彇鍐充簬
A
锛庢阿閿�佺洂閿�佽寖寰峰崕鍔涘拰鐤忔按鍔涚瓑鏋勮薄缁寸郴鍔?/p>
B
锛庤偨閾鹃棿鍙婅偨閾惧唴鐨勪簩纭敭
C
锛庢皑鍩洪吀鐨勭粍鎴愩�侀『搴忓拰鏁扮洰
D
锛庢俯搴︺�?/p>
pH
鍜岀瀛愬己搴︾瓑鐜鏉′欢
2
锛?/p>
鐪熸牳鐢熺墿
mRNA
鐨勫附瀛愮粨鏋勬槸閫氳繃涓�涓?/p>
m
7
G
鍔犲埌鏂扮敓鐨?/p>
RNA
杞綍鐗╀笂浜х敓鐨勶紝
鍏?/p>
杩炴帴鏂瑰紡鏄?/p>
A
锛?/p>
3
锛囷紞
5
锛?/p>
B
锛?/p>
5
锛囷紞
3
锛?/p>
C
锛?/p>
5
锛囷紞
5
锛?/p>
D
锛?/p>
5
锛囷紞
3
锛?/p>
3
锛?/p>
DNA
鍏嬮殕涓嶅寘鎷笅鍒楀摢椤规楠?/p>
A
锛?/p>
閲嶇粍浣撶敤铻嶅悎娉曞鍏ョ粏鑳?/p>
B
锛?/p>
鐩?/p>
鐨勫熀鍥犵殑鑾峰彇
C
锛?/p>
閫?/p>
鎷╀竴涓悎閫傜殑杞戒綋
D
锛?/p>
鐢ㄨ繛鎺ラ叾杩炴帴杞戒綋
DNA
鍜岀洰鐨?/p>
DNA
褰㈡垚閲嶇粍
DNA
4
锛庣背姘忓父鏁板湪鎺ㄥ杩囩▼涓紩鍏ヤ簡鍝」鍋囪
A
锛庡拷鐣ュ弽搴?/p>
ES
E+S
瀛樺湪
B
锛庨叾娴撳害涓哄簳鐗╂祿搴︾殑涓�鍗?/p>
C
锛庣敱浜?/p>
ES
鐨勫瓨鍦ㄤ娇搴曠墿鍒濆娴撳害闄嶄綆
D
锛庣敱浜?/p>
P
0
锛屾墍浠ヤ笉鑰冭檻鍙嶅簲
E+P
ES
鐨勫瓨鍦?/p>
5
锛庡鏋滃皢鐞ョ弨閰?/p>
(
寤惰儭缇ч吀
/
鐞ョ弨閰告哀鍖栬繕鍘熺數浣?/p>
+0.03V)
鍔犲埌纭吀閾佸拰纭吀浜氶搧
(
楂橀搧
/
浜氶搧姘у寲杩樺師鐢典綅
+0.077V)
鐨勫钩琛℃憾娑蹭腑锛屽彲鑳藉彂鐢熺殑鍙樺寲鏄?/p>
A
锛庣～閰镐簹閾佸拰寤惰儭缇ч吀鐨勬祿搴﹀皢澧炲姞
B
锛庣～閰搁搧鐨勬祿搴﹀皢澧炲姞