M13
鍣弻浣撶┖鏂戝疄楠?/p>
涓�銆佸疄楠岀洰鐨勶細
1
銆佷簡瑙?/p>
M13
鍣弻浣撹浇浣撶殑涓�鑸粨鏋勩�佹�ц川鍙婃劅鏌撶壒寰併�?/p>
2
銆佸涔犲苟鎺屾彙
M13
鍣弻浣撹浇浣撶殑绌烘枒绾寲鎶�鏈�?/p>
浜屻�佸疄楠屽師鐞嗭細涓�涓?/p>
M13
鍣弻浣撶殑鐥呮瘨棰楃矑鎰熸煋涓�涓粏鑿屽悗褰㈡垚涓�涓櫖鑿屾枒銆備粠鎰熸煋缁嗚弻
閲婃斁鍑烘潵鐨勫瓙浠ｇ梾姣掗绮掓劅鏌撲复杩戠粏鑿岋紝
鍚庤�呭張鍙噴鏀句笅涓�浠ｇ梾姣掗绮掞紱
濡傛灉缁嗚弻鍦ㄥ崐鍥轰綋
鍩瑰吇鍩轰笂鐢熼暱锛?/p>
鑷甫鐥呮瘨棰楃矑鐨勬墿鏁ｅ彈鍒颁竴瀹氶檺鍒讹紝
璇稿
M13
绛変笣鐘跺櫖鑿屼綋骞朵笉瑁傝В缁嗚弻锛?/p>
浣嗗彲浠ヤ娇缁嗚弻鐢熼暱閫熷害闄嶄綆鑷冲師鏉ョ殑
1/2
锛屽湪涓婂眰鐞艰剛涓敓闀跨殑鑿屽彴鐨勮儗鏅笅锛岀敓闀跨紦鎱㈢粏
鑿屽舰鎴愪簡涓�涓笉鏂墿澶у埌鑲夌溂鍙鐨勫櫖鑿屾枒銆?/p>
涓夈�佹潗鏂欎笌璇曞墏锛?/p>
1
銆?/p>
M13
鍣弻浣?/p>
2
銆?/p>
ER2738
鑿岀
3
銆?/p>
LB
鍩瑰吇鍩?/p>
鍥涖�佸疄楠屾楠?/p>
1.
1
銆佸彇
5ml LB
娑蹭綋鍩瑰吇鍩轰簬
10ml
鐨勮瘯绠′腑锛屽姞鍏?/p>
5ul
鍥涚幆绱狅紙
20mg/ml
锛?/p>
锛屽皢澶ц偁鏉嗚弻
鎺ュ叆锛?/p>
3
7
鈩冮渿鑽★紙
80-100rpm
锛夊煿鍏昏繃澶滐紙
12h
锛?/p>
2.
灏?/p>
2ml
鐨?/p>
ER2738
鍩瑰吇娑茶浆鍏?/p>
20ml
鐨?/p>
LB
鍩瑰吇鍩轰腑锛?/p>
1:10
绋�閲婏級
锛岀户缁?/p>
3
7
鈩冮渿鑽?/p>
锛?/p>
80-100rpm
锛夊煿鍏?/p>
4.5h
锛堜笉瀹滆繃蹇紝鍚﹀垯锛岀粏鑿屾�ф瘺琚牬鍧忥紝鍣弻浣撴棤娉曞鍏ワ級
锛屽嵆
鎴愪负鎰熷彈鎬佺粏鑳?/p>
3.
鍩瑰吇缁嗚弻鏃跺皢姘存荡閿呮墦寮�锛?/p>
43
鈩冮鐑紝骞跺皢
LB
骞虫澘缃簬
37
鈩冮鐑鐢?/p>
4.
鐢ㄨ鏈?/p>
LB
鍩瑰吇鍩虹殑
EP
绠?/p>
10
鍊嶇█閲婂櫖鑿屼綋锛?/p>
鐞嗘兂鐨勭█閲婅寖鍥达細
鎵╁杩囩殑鍣弻浣?/p>
10
8
-10
10
鍊嶇█閲婏紝鏈墿澧炶繃鐨?/p>
10
4
-10
5
鍊嶇█閲?/p>
5.
灏嗛《灞傜惣鑴傜敤寰尝鐐夋垨鑰呯數纾佺倝娌告按铻嶅寲锛岀疆浜庢按娴撮攨涓繚娓╁緟鐢?/p>
6.
褰撳ぇ鑲犳潌鑿岃揪鍒板鏁版垚闀挎湡锛屽彇澶ц偁鏉嗚弻
ER2738
鎰熷彈鎬佺粏鑳?/p>
200ul
浜?/p>
EP
绠′腑
7.
鍔犲叆
10ul-100
ul
锛?/p>
鎴栬�呬竴瀹氫綋绉級
涓�瀹氱█閲婂害鐨勫櫖鑿屼綋婧舵恫锛?/p>
蹇�熸贩鍚堬紝
瀹ゆ俯瀛佃偛
1-5min
8.
灏嗘劅鏌撹繃鐨勫ぇ鑲犳潌鑿岀殑鍩瑰吇娑插�掑叆
43
鈩冮《灞傜惣鑴備腑锛岀敤
vortex
杩呴�熸贩鍖�锛岀珛鍒诲湪棰勭儹
鐨?/p>
LB
鍩瑰吇鍩轰笂鍊掑钩鏉匡紝杩呴�熸憞鏅冨钩鏉夸娇椤跺眰鐞艰剛鍧囧寑灞曞紑骞堕摵婊¤〃闈?/p>
9.
瀹ゆ俯闈欑疆
5min
宸﹀彸浣垮钩鏉垮喎鍗村嚌鍥猴紝灏嗗煿鍏荤毧鍊掔疆
37
鈩冨煿鍏昏繃澶?/p>
10.
绗簩澶╄瀵熷钩鏉匡紝瀵瑰煿鍏荤毧涓殑鍣弻鏂戣繘琛岃鏁帮紝璁＄畻绾﹀惈鏈?/p>
10-100
涓暟閲忕骇鐨勫钩鏉?
鍣弻鏂戠殑鍏蜂綋鏁扮洰锛?/p>
y
锛?/p>
锛岃绠楀櫖鑿屼綋鏁堜环锛?/p>
pfu/ml
锛夊櫖鑿屼綋鏁堜环
=y*10
x+2
puf/ml