铔嬬櫧璐ㄥ嵃杩癸紙
Western blot
锛夊疄楠屾柟妗?/p>
婧舵恫鍜岃瘯鍓?/p>
瑁傝В缂撳啿娑?/p>
杩欎簺缂撳啿娑插彲鍦?/p>
4
鈩?/p>
涓嬩繚瀛樻暟鍛紝鎴栬�呬互鍒嗚褰㈠紡鍦?/p>
-20
鈩?/p>
涓嬩繚瀛橀暱杈?/p>
1
骞淬�?/p>
Nonidet-P40 (NP40)
缂撳啿娑?/p>
150 mM NaCl
1.0% NP40
锛堝彲鐢?/p>
0.1% Triton X-100
鏇夸唬锛?/p>
50 mM Tris-HCl pH 8.0
铔嬬櫧閰舵姂鍒跺墏
RIPA
缂撳啿娑诧紙鏀惧皠鍏嶇柅娌夋穩璇曢獙缂撳啿娑诧級
150 mM NaCl
1.0% NP-40
鎴?/p>
0.1% Triton X-100
0.5%
鑴辨哀鑳嗛吀閽?/p>
0.1% SDS
锛堝崄浜岀兎鍩虹～閰搁挔锛?/p>
50 mM Tris-HCl pH 8.0
铔嬬櫧閰舵姂鍒跺墏
Tris-HCl
缂撳啿娑?/p>
20 mM Tris-HCl pH 7.5
铔嬬櫧閰舵姂鍒跺墏
鐢垫吵銆佽浆鑶溿�佸皝闂紦鍐叉恫
Laemmli 2脳
缂撳啿娑?/p>
/
涓婃牱缂撳啿娑?/p>
4% SDS
10% 2-
宸熀涔欓唶
20%
鐢樻补
0.004%
婧撮厷钃?/p>
0.125 M Tris-HCl
娴嬪畾
pH
骞惰皟鑺傝嚦
pH 6.8
銆?/p>
鐢垫吵缂撳啿娑诧紙
Tris-
鐢樻皑閰?/p>
/SDS
锛?/p>
25 mM Tris
纰?/p>
190 mM
鐢樻皑閰?/p>
0.1% SDS
娴嬪畾
pH
锛?/p>
pH
搴斾负绾?/p>
8.3
銆傚繀瑕佹椂杩涜璋冭妭銆?/p>
杞啘缂撳啿娑诧紙婀胯浆锛?/p>
25 mM Tris
纰?/p>
190 mM
鐢樻皑閰?/p>
20%
鐢查唶
娴嬪畾
pH
锛?/p>
pH
搴斾负绾?/p>
8.3
銆傚繀瑕佹椂杩涜璋冭妭銆?/p>
瀵逛簬澶т簬
80 kDa
鐨勮泲鐧借川锛屾垜浠帹鑽愬姞鍏ユ渶缁堟祿搴︿负
0.1%
鐨?/p>
SDS
銆?/p>
杞啘缂撳啿娑诧紙鍗婂共杞級