GST
浜插拰灞傛瀽浠嬭川浣跨敤璇存槑涔?/p>
涓�銆?/p>
绠�浠?/p>
GST
浜插拰灞傛瀽浠嬭川锛?/p>
GST Agarose
锛夋槸涓撻棬璁捐鐢ㄤ簬绾寲璋疯儽鐢樿偨
S-
杞Щ閰讹紙
GST
锛夎瀺鍚堣泲鐧姐�佸叾瀹冭胺鑳辩敇鑲借浆绉婚叾
浠ュ強涓庤胺鑳辩敇鑲芥湁浜插拰浣滅敤铔嬬櫧鐨勫垎绂讳粙璐紝涓�姝ュ垎绂诲氨鍙緱鍒伴珮绾害鐨?/p>
GST
铻嶅悎鐩爣铔嬬櫧锛岀函鍖栨潯浠舵俯鍜岋紝鍙互淇?/p>
璇佽泲鐧界殑娲绘�с�?/p>
鏈骇鍝佹槸鑷富璁捐鍚堟垚鐨?/p>
GST
鐞艰剛绯栧嚌鑳讹紝鍏锋湁浼樿壇鐨勭墿鐞嗗拰鍖栧绋冲畾鎬э紝浣跨敤瀵垮懡闀匡紝鎿嶄綔鏂逛究锛屾壒娆￠噸澶嶆�уソ锛?/p>
鏄撲簬鏀惧ぇ锛屾槸鐮斿彂涓庣敓浜х殑鐞嗘兂閫夋嫨銆?/p>
浜屻�?/p>
鎬ц兘鍙傛暟
鐗圭偣
鍩哄洟瀵嗗害楂橈紝杞介噺澶?/p>
鍩鸿川
6%
鐨勪氦鑱旂惣鑴傜硸鍑濊兌
鍚搁檮杞介噺
鈮?5mg GST
铻嶅悎铔嬬櫧锛?/p>
55kDa
锛?/p>
/ml
浠嬭川骞冲潎绮掑緞
100渭m
鏈�澶ф祦閫?/p>
300cm/h
pH
鑼冨洿
3~10
锛屽湪浣嶆竻娲楁椂
pH
鑼冨洿鍙埌
2~11
浣跨敤娓╁害
甯告俯
淇濆瓨娓╁害
+4~8
鈩?/p>
淇濆瓨娑蹭綋
20%
涔欓唶
鍖栧绋冲畾鎬?/p>
瀹ゆ俯涓嬪彲鍦?/p>
0.1M
鏌犳閰?/p>
(pH 4.0)
锛?/p>
0.1 M NaOH
锛?/p>
70%
涔欓唶鎴?/p>
6M
鐩愰吀鑳嶄腑鑰愬彈
2h
锛岃泲鐧界粨鍚堣兘鍔涗笉鍙楀奖鍝?/p>
涓夈�?/p>
閫傜敤鑼冨洿
鍒嗙璋疯儽鐢樿偨
S-
杞Щ閰讹紙
GST
锛夎瀺鍚堣泲鐧姐�佸叾瀹冭胺鑳辩敇鑲借浆绉婚叾浠ュ強涓庤胺鑳辩敇鑲芥湁浜插拰浣滅敤鐨勮泲鐧姐�?/p>
鍥涖�?/p>
鎿嶄綔璇存槑
1.
缂撳啿娑查厤鍒?/p>
缂撳啿娑?/p>
A
锛堝钩琛＄紦鍐叉恫锛夛細
10mM Na
2
HPO
4
锛?/p>
1.8mM KH
2
PO
4
锛?/p>
140mM NaCl
锛?/p>
2.7mM KCl
锛岃皟鑺?/p>
pH
鍊艰嚦
8.0
銆?/p>
缂撳啿娑?/p>
B
锛堟礂鑴辩紦鍐叉恫锛夛細
10mM Glutathione
锛堣繕鍘熷瀷锛夛紝
50mM Tris-HCl
锛岃皟鑺?/p>
pH
鍊艰嚦
8.0
銆傚洜
Glutathione
鏄?/p>
姘?/p>
鍖栵紝闇�鐜扮敤鐜伴厤銆?/p>
锛堟敞锛氬悇绉嶆憾娑查厤鍒跺畬姣曞悗锛屾渶濂借繘琛岃劚姘斿鐞嗭紝
0.45 渭m
婊よ啘杩囨护澶囩敤
)
銆?/p>
2.
鏍峰搧棰勫鐞嗭細