BCA
铔嬬櫧娴撳害娴嬪畾
Pierce
庐
BCA Protein Assay Kit
(
璇曞墏鐩掑娓╀繚瀛?/p>
)
1.
鍑嗗
BSA
鏍囧噯鍝?/p>
鎸?/p>
Table 1
閰嶅埗涓�绯诲垪鐨勮泲鐧芥爣鍑嗗搧
.
鍘熸恫
(stock)
:
鍙栧嚭涓�涓?/p>
ample(
鍐呭惈
2mg/mL
鐨勭櫧铔嬬櫧鏍囧噯鍝?/p>
(
姝ら噺瓒冲
3
涓?/p>
replication)
2.
鍑嗗
BCA
宸ヤ綔娑?/p>
(Working Reagent,WR)
(1)
鐢ㄥ叕寮忚绠楁�诲伐浣滄恫閲?/p>
(
鏍囧噯鍝佹暟
+
寰呮祴鏍峰搧鏁?/p>
)*(
澶嶅埗鏁?/p>
)*(
姣忎釜宸ヤ綔娑茬殑閲?/p>
)=
鎬荤殑闇�瑕佺殑宸ヤ綔娑?/p>
鐨勯噺
渚嬪瓙
:3
涓緟娴嬫牱鍝?/p>
,
姣忎釜鏍峰搧
2
涓鍒舵墍闇�鐨勫伐浣滄恫閲?/p>
(9
涓爣鍑嗗搧
+3
涓緟娴嬫牱鍝?/p>
)*(2
涓鍒舵暟
)*(2ml)=48ml
宸ヤ綔娑?/p>
Note:
璇曠娉?/p>
:
姣忎釜璇曠闇�
2ml
宸ヤ綔娑?/p>
寰瓟鏉?/p>
:
姣忎釜瀛旈渶
200ul
宸ヤ綔娑?/p>
(
渚嬪瓙涓渶鍏?/p>
4.8ml)
(2)BCA
璇曞墏
A:B=50:1
閰嶅埗閫傞噺
BCA
宸ヤ綔娑诧紝鍏呭垎娣峰寑銆?/p>
瀵逛簬涓婇潰鐨勪緥瀛?/p>
,
璇曠娉曢渶瑕?/p>
50ml BCA A
娑?/p>
+1ml BCA B
娑?/p>
,
寰瓟鏉挎硶闇�
5ml A
娑?/p>
鍜?/p>
1mlB
娑?/p>
.