鑶滆泲鐧借浆杩愭満鍒跺強鐩稿叧铔嬬櫧璐ㄥ姛鑳界爺绌?- 鐧惧害鏂囧簱

鏂板缓

涓婁紶

棣栭〉

鍔╂墜

鏈�杩?/div>

璧勬枡搴?/div>

宸ュ叿

鑶滆泲鐧借浆杩愭満鍒跺強鐩稿叧铔嬬櫧璐ㄥ姛鑳界爺绌?/p>

 

鎽樿�佺粏鑳炲垎娉屾槸鍩烘湰鐨勭敓鍛芥椿鍔�锛?/p>

鏄�鐢熺墿淇℃伅浼犻�掔殑鍏抽敭鐜�鑺傘�?/p>

鍏嶇柅闃插崼鍙嶅簲銆?/p>

缁嗚優鐨勭敓闀裤�?/p>

鍙楃簿杩囩▼銆?/p>

缁嗚優鍐呭�栫殑鐗╄川浜ゆ崲銆?/p>

缁嗚優鑶滀笂鍙椾綋铔嬬櫧鐨勫祵鍏ヤ互鍙婂啀寰�鐜�绛夌粏鑳炵敓鐞嗗姛鑳界殑瀹?/p>

鐜伴兘涓庣粏鑳炲垎娉屾椿鍔ㄦ湁鍏筹紝

涓旀墍娑夊強鐨勪竴浜涜啘杞�杩愯泲鐧藉拰鍒嗗瓙鏈哄埗鏄�绫讳技鐨勩�?/p>

鏂囩珷瀵瑰垎娉岃繃

绋嬨�?/p>

鐩稿叧铔嬬櫧璐ㄧ殑鍔熻兘浠ュ強瀵瑰垎娉岀殑璋冩帶绛夎繘琛屼簡姒傝堪锛?/p>

浠嬬粛浜嗗浗鍐呭�栧�硅啘铔嬬櫧杞�杩愭満鍒跺�?/p>

鍏剁浉鍏宠泲鐧借川鍔熻兘鐨勬渶鏂扮爺绌舵垚鏋滐紝骞跺�圭洰鍓嶆彁鍑虹殑鍋囪�惧強瀛樺湪鐨勯棶棰樿繘琛屼簡璁ㄨ�轰笌灞曟湜銆?/p>

 

鍏抽敭璇嶇粏鑳炲垎娉屽泭娉¤浆杩愯優鍚炶啘铔嬬櫧

 

 

鍥婃场杞�杩愬拰鍒嗘硨杩囩▼鏄�鐢熷懡娲诲姩鐨勫熀鏈�浜嬩欢涔嬩竴锛?/p>

鏄�绁炵粡淇″彿浼犲�煎拰鍐呭垎娉屾縺绱犻噴鏀剧殑

鍩虹��銆傚垎娉岃繃绋嬫槸涓�涓�杩炵画鐨勫姩鎬佸惊鐜�锛屽寘鎷�鍥婃场鍦ㄧ粏鑳炲唴鐨勭敓鎴愩�佸嫙闆嗐�佹嫶绯汇�侀敋瀹氬拰婵�

娲伙紝

浠ュ強涓庣粏鑳炶啘铻嶅悎褰㈡垚铻嶅悎瀛斿苟灏嗗唴瀹圭墿閲婃斁鍒拌優澶栫瓑姝ラ�わ�?/p>

鍏跺悗浼撮殢鐫�鍥婃场鐨勮優鍚炲拰

寰�鐜�鍐嶅埄鐢ㄣ�?/p>

鐩�鍓嶅浗闄呬笂褰㈡垚浜嗕竴涓�鐮旂┒鑳炲唴铔嬬櫧璐ㄤ笌鑶滆浆杩愩�?/p>

鍥婃场涓庣粏鑳炶川鑶滅殑铻嶅悎鍙婄粏

鑳炵殑鍒嗘硨鍜岃優鍚炶繃绋嬩腑鍒嗗瓙鏈哄埗涓庤皟鑺傞�斿緞鐨勭儹鐐癸紝

杩欏�逛簬鎻�绀虹�炵粡淇″彿浼犻�掋�?/p>

瀛︿範涓庤�板繂銆?/p>

鍐呭垎娉屾縺绱犲垎娉屻�?/p>

缁嗚優鐨勭敓闀裤�?/p>

鍏嶇柅缁嗚優鑴遍�楃矑銆?/p>

鑶滀笂鍔熻兘铔嬬櫧鐨勫祵鍏ョ瓑閲嶈�佺敓鍛借繃绋嬫湁鐫�

鏋佸叾閲嶈�佺殑鎰忎箟銆?/p>

 

1

鍥婃场寰�鐜�姒傝堪

 

鍒嗘硨娲诲姩涓�锛�

鍥婃场婧愯嚜浜庝粠鍐呰川缃戝埌楂樺皵鍩轰綋鐨勮繛缁�杩愯緭杩囩▼锛�

骞朵笖瑕佺粡杩囦竴涓�澶嶆潅鐨勫姩鎬?/p>

寰�鐜�锛?/p>

灏界�″垎娉岃繃绋嬪悇姝ラ�や箣闂撮毦浠ユ埅鐒跺垎寮�锛?/p>

浣嗗悇姝ラ�や腑鍙備笌鍙嶅簲鐨勫垎瀛愭湁鎵�涓嶅悓锛?/p>

鎵�鍙?/p>

鍒扮殑璋冩帶涔熶笉鍚岋紝鍥犺�屽彲灏嗘暣涓�杩囩▼鍒嗕负涓嶅悓鐨勫姛鑳介樁娈点�?/p>

 

1

锛?/p>

1

鍥婃场鐨勫舰鎴?/p>

 

鍦ㄧ粏鑳炰腑鏈夊�氱�嶉�斿緞褰㈡垚鍥婃场锛?/p>

鍒嗘硨鍥婃场鍙�浠ョ敱鍐呰川缃戞垨鑰呴珮灏斿熀浣撳嚭鑺戒骇鐢燂紝

涔熷彲浠ラ�氱敓锛?/p>

鍐呮兜浣擄紙

endo-some

锛変篃鍙�浠ュ垎閫夊嚭鏂扮殑鍥婃场銆傚垎娉岃泲鐧藉湪鍐呰川缃戜腑鍚堟垚浠ュ悗锛屽唴璐ㄧ綉浼氬舰

鎴愬惈鏈夎繖浜涜泲鐧界殑杞�杩愬泭娉★紝骞剁Щ鍔ㄥ埌椤哄紡楂樺皵鍩轰綋锛�

cisgolgireticulum

锛?/p>

CGN

锛夌殑鑶滃眰锛屽埌

杈鹃珮灏斿熀鐨勯『闈�锛�

cisface

锛?/p>

銆傝繖浜涜泲鐧界Щ鍔ㄧ┛杩囬珮灏斿熀澶嶅悎浣撳埌杈惧弽闈�锛�

transface

锛?/p>

锛岃繘

 

鍏ュ弽寮忛珮灏斿熀浣擄紙

tansgolgireticulum

锛?/p>

TGN

锛?/p>

锛屽湪杩欓噷锛岃泲鐧借川琚�鍒嗛�夊埌涓ょ被涓嶅悓鐨勫泭娉′腑銆?/p>

涓�绫诲泭娉＄洿鎺ヤ笌缁嗚優鑶滆瀺鍚堝苟灏嗗叾鍐呭�圭墿锛堝�傝兌鍘熸垨琛�娴嗚泲鐧界瓑鍒嗘硨铔嬬櫧锛夐噴鏀惧埌缁嗚優澶栵紱

鑰屽ぇ澶氭暟鍒嗘硨铔嬬櫧鐨勫垎娉屾槸涓嶈繛缁�鐨勶紝杩欎簺铔嬬櫧鍦�

TGN

涓�琚�鍒嗛�夊埌鍙︿竴绫诲泭娉￠噷锛岃繖绫诲泭

娉″彧鏈夌瓑鍙楀埌鍒烘縺鏃舵墠鍦ㄧ粏鑳炲唴杞�杩愬苟鍒嗘硨锛�

渚嬪�傚垎娉屾縺绱犵殑缁嗚優鏄�鐢变笉鍚岀殑绁炵粡閫掕川鍜屾縺

绱犲埡婵�寮曡捣鐨勩�?/p>

 

1

锛?/p>

2

鍥婃场鐨勫嫙闆?/p>

 

鍥婃场鍕熼泦鏄�鑳炴祮鍐呭泭娉″悜缁嗚優鍛ㄨ竟閮ㄤ綅瀹氬悜杞�杩愮殑杩囩▼銆?/p>

鍏跺垎瀛愭満鍒舵秹鍙婂寘鎷�鍔ㄥ姏铔嬬櫧銆?/p>

Rab

铔嬬櫧鍜?/p>

SNARE

铔嬬櫧绛夊湪鍐呯殑澶氱�嶅泭娉¤泲鐧戒笌缁嗚優楠ㄦ灦鐨勭浉浜掍綔鐢ㄤ互鍙婂�氱�嶈皟鑺傚洜瀛愮殑璋冩帶銆?/p>

浠庨珮灏斿熀浣撳埌缁嗚優鍛ㄨ竟閮ㄤ綅鐨勫泭娉¤浆杩愪緷璧栦簬寰�绠＄郴缁燂�?/p>

 

ATP

鎻愪緵浜嗗泭娉¤繍杈撴墍闇�瑕佺殑鑳介噺銆?/p>

 

1

锛?/p>

3

鍥婃场鐨勬嫶绯诲拰閿氬畾

 

鎷寸郴鏄�鎸囧泭娉℃澗鏁ｅ湴闄勭潃浜庨澏鑶滅殑杩囩▼鎴栫姸鎬併�?/p>

鎷寸郴鐘舵�佷笅锛?/p>

鍥婃场鑶滀笌闈惰啘鐨勮窛绂荤害涓?/p>

75

锝?/p>

150nm

銆?/p>

鎷寸郴鍚庡泭娉¤繘涓�姝ラ潬杩戦澏鑶滐紝

涓ゅ眰鑶滀箣闂寸殑璺濈�荤害涓�

5

锝?/p>

10nm

锛?/p>

姝よ繃绋嬫垨鐘舵�佸嵆鍥?/p>

娉＄殑閿氬畾銆?/p>

鍥婃场鐨勬嫶绯诲拰閿氬畾瀵逛繚璇佸泭娉′笌闈惰啘鐨勭壒寮傛�ц瀺鍚堛�?/p>

浠ュ強缁嗚優鍦ㄦ�ｇ‘鐨勬椂闂村拰绌�

闂村唴杩涜�岃啘涓庤泲鐧借川鐨勮浆杩愬叿鏈夐噸瑕佹剰涔夛�?/p>

瀵圭淮鎸佸悇缁嗚優鍣ㄧ殑鐗瑰紓鎬т篃鍗佸垎鍏抽敭銆?/p>

鍥婃场浠庢嫶

绯荤姸鎬佽浆鍙樹负閿氬畾鐘舵�佺殑鍒嗗瓙鏈哄埗鐩�鍓嶈繕涓嶆竻妤氥�?/p>

涓�绉嶅彲鑳芥槸鎷寸郴澶嶅悎鐗╁湪涓庡泭娉＄粨鍚堝悗鍙?/p>

鐢熷ぇ鐨勬瀯璞℃敼鍙橈紝鐗垫媺鍥婃场浣夸箣闈犺繎闈惰啘锛涘彟涓�绉嶅彲鑳芥槸

Sec1

锛?/p>

Munc18

铔嬬櫧涓?/p>

Rab

铔嬬櫧鏁?/p>

搴旂墿鍙婃嫶绯诲�嶅悎鐗╂垨鍏跺畠缁嗚優鑶滆泲鐧界浉浜掍綔鐢�锛屼績杩涘泭娉′粠鎷寸郴鐘舵�佽浆鍙樹负閿氬畾鐘舵�併�?/p>

 

1

锛?/p>

4

鍥婃场鐨勬縺娲?/p>