鑶滆泲鐧借浆杩愭満鍒跺強鐩稿叧铔嬬櫧璐ㄥ姛鑳界爺绌?/p>
鎽樿缁嗚優鍒嗘硨鏄熀鏈殑鐢熷懡娲诲姩锛?/p>
鏄敓鐗╀俊鎭紶閫掔殑鍏抽敭鐜妭銆?/p>
鍏嶇柅闃插崼鍙嶅簲銆?/p>
缁嗚優鐨勭敓闀裤�?/p>
鍙楃簿杩囩▼銆?/p>
缁嗚優鍐呭鐨勭墿璐ㄤ氦鎹€�?/p>
缁嗚優鑶滀笂鍙椾綋铔嬬櫧鐨勫祵鍏ヤ互鍙婂啀寰幆绛夌粏鑳炵敓鐞嗗姛鑳界殑瀹?/p>
鐜伴兘涓庣粏鑳炲垎娉屾椿鍔ㄦ湁鍏筹紝
涓旀墍娑夊強鐨勪竴浜涜啘杞繍铔嬬櫧鍜屽垎瀛愭満鍒舵槸绫讳技鐨勩�?/p>
鏂囩珷瀵瑰垎娉岃繃
绋嬨�?/p>
鐩稿叧铔嬬櫧璐ㄧ殑鍔熻兘浠ュ強瀵瑰垎娉岀殑璋冩帶绛夎繘琛屼簡姒傝堪锛?/p>
浠嬬粛浜嗗浗鍐呭瀵硅啘铔嬬櫧杞繍鏈哄埗鍙?/p>
鍏剁浉鍏宠泲鐧借川鍔熻兘鐨勬渶鏂扮爺绌舵垚鏋滐紝骞跺鐩墠鎻愬嚭鐨勫亣璁惧強瀛樺湪鐨勯棶棰樿繘琛屼簡璁ㄨ涓庡睍鏈涖�?/p>
鍏抽敭璇嶇粏鑳炲垎娉屽泭娉¤浆杩愯優鍚炶啘铔嬬櫧
鍥婃场杞繍鍜屽垎娉岃繃绋嬫槸鐢熷懡娲诲姩鐨勫熀鏈簨浠朵箣涓�锛?/p>
鏄缁忎俊鍙蜂紶瀵煎拰鍐呭垎娉屾縺绱犻噴鏀剧殑
鍩虹銆傚垎娉岃繃绋嬫槸涓�涓繛缁殑鍔ㄦ�佸惊鐜紝鍖呮嫭鍥婃场鍦ㄧ粏鑳炲唴鐨勭敓鎴愩�佸嫙闆嗐�佹嫶绯汇�侀敋瀹氬拰婵�
娲伙紝
浠ュ強涓庣粏鑳炶啘铻嶅悎褰㈡垚铻嶅悎瀛斿苟灏嗗唴瀹圭墿閲婃斁鍒拌優澶栫瓑姝ラ锛?/p>
鍏跺悗浼撮殢鐫�鍥婃场鐨勮優鍚炲拰
寰幆鍐嶅埄鐢ㄣ�?/p>
鐩墠鍥介檯涓婂舰鎴愪簡涓�涓爺绌惰優鍐呰泲鐧借川涓庤啘杞繍銆?/p>
鍥婃场涓庣粏鑳炶川鑶滅殑铻嶅悎鍙婄粏
鑳炵殑鍒嗘硨鍜岃優鍚炶繃绋嬩腑鍒嗗瓙鏈哄埗涓庤皟鑺傞�斿緞鐨勭儹鐐癸紝
杩欏浜庢彮绀虹缁忎俊鍙蜂紶閫掋�?/p>
瀛︿範涓庤蹇嗐�?/p>
鍐呭垎娉屾縺绱犲垎娉屻�?/p>
缁嗚優鐨勭敓闀裤�?/p>
鍏嶇柅缁嗚優鑴遍绮掋�?/p>
鑶滀笂鍔熻兘铔嬬櫧鐨勫祵鍏ョ瓑閲嶈鐢熷懡杩囩▼鏈夌潃
鏋佸叾閲嶈鐨勬剰涔夈�?/p>
1
鍥婃场寰幆姒傝堪
鍒嗘硨娲诲姩涓紝
鍥婃场婧愯嚜浜庝粠鍐呰川缃戝埌楂樺皵鍩轰綋鐨勮繛缁繍杈撹繃绋嬶紝
骞朵笖瑕佺粡杩囦竴涓鏉傜殑鍔ㄦ�?/p>
寰幆锛?/p>
灏界鍒嗘硨杩囩▼鍚勬楠や箣闂撮毦浠ユ埅鐒跺垎寮�锛?/p>
浣嗗悇姝ラ涓弬涓庡弽搴旂殑鍒嗗瓙鏈夋墍涓嶅悓锛?/p>
鎵�鍙?/p>
鍒扮殑璋冩帶涔熶笉鍚岋紝鍥犺�屽彲灏嗘暣涓繃绋嬪垎涓轰笉鍚岀殑鍔熻兘闃舵銆?/p>
1
锛?/p>
1
鍥婃场鐨勫舰鎴?/p>
鍦ㄧ粏鑳炰腑鏈夊绉嶉�斿緞褰㈡垚鍥婃场锛?/p>
鍒嗘硨鍥婃场鍙互鐢卞唴璐ㄧ綉鎴栬�呴珮灏斿熀浣撳嚭鑺戒骇鐢燂紝
涔熷彲浠ラ�氱敓锛?/p>
鍐呮兜浣擄紙
endo-some
锛変篃鍙互鍒嗛�夊嚭鏂扮殑鍥婃场銆傚垎娉岃泲鐧藉湪鍐呰川缃戜腑鍚堟垚浠ュ悗锛屽唴璐ㄧ綉浼氬舰
鎴愬惈鏈夎繖浜涜泲鐧界殑杞繍鍥婃场锛屽苟绉诲姩鍒伴『寮忛珮灏斿熀浣擄紙
cisgolgireticulum
锛?/p>
CGN
锛夌殑鑶滃眰锛屽埌
杈鹃珮灏斿熀鐨勯『闈紙
cisface
锛?/p>
銆傝繖浜涜泲鐧界Щ鍔ㄧ┛杩囬珮灏斿熀澶嶅悎浣撳埌杈惧弽闈紙
transface
锛?/p>
锛岃繘
鍏ュ弽寮忛珮灏斿熀浣擄紙
tansgolgireticulum
锛?/p>
TGN
锛?/p>
锛屽湪杩欓噷锛岃泲鐧借川琚垎閫夊埌涓ょ被涓嶅悓鐨勫泭娉′腑銆?/p>
涓�绫诲泭娉＄洿鎺ヤ笌缁嗚優鑶滆瀺鍚堝苟灏嗗叾鍐呭鐗╋紙濡傝兌鍘熸垨琛�娴嗚泲鐧界瓑鍒嗘硨铔嬬櫧锛夐噴鏀惧埌缁嗚優澶栵紱
鑰屽ぇ澶氭暟鍒嗘硨铔嬬櫧鐨勫垎娉屾槸涓嶈繛缁殑锛岃繖浜涜泲鐧藉湪
TGN
涓鍒嗛�夊埌鍙︿竴绫诲泭娉￠噷锛岃繖绫诲泭
娉″彧鏈夌瓑鍙楀埌鍒烘縺鏃舵墠鍦ㄧ粏鑳炲唴杞繍骞跺垎娉岋紝
渚嬪鍒嗘硨婵�绱犵殑缁嗚優鏄敱涓嶅悓鐨勭缁忛�掕川鍜屾縺
绱犲埡婵�寮曡捣鐨勩�?/p>
1
锛?/p>
2
鍥婃场鐨勫嫙闆?/p>
鍥婃场鍕熼泦鏄優娴嗗唴鍥婃场鍚戠粏鑳炲懆杈归儴浣嶅畾鍚戣浆杩愮殑杩囩▼銆?/p>
鍏跺垎瀛愭満鍒舵秹鍙婂寘鎷姩鍔涜泲鐧姐�?/p>
Rab
铔嬬櫧鍜?/p>
SNARE
铔嬬櫧绛夊湪鍐呯殑澶氱鍥婃场铔嬬櫧涓庣粏鑳為鏋剁殑鐩镐簰浣滅敤浠ュ強澶氱璋冭妭鍥犲瓙鐨勮皟鎺с�?/p>
浠庨珮灏斿熀浣撳埌缁嗚優鍛ㄨ竟閮ㄤ綅鐨勫泭娉¤浆杩愪緷璧栦簬寰绯荤粺锛?/p>
ATP
鎻愪緵浜嗗泭娉¤繍杈撴墍闇�瑕佺殑鑳介噺銆?/p>
1
锛?/p>
3
鍥婃场鐨勬嫶绯诲拰閿氬畾
鎷寸郴鏄寚鍥婃场鏉炬暎鍦伴檮鐫�浜庨澏鑶滅殑杩囩▼鎴栫姸鎬併�?/p>
鎷寸郴鐘舵�佷笅锛?/p>
鍥婃场鑶滀笌闈惰啘鐨勮窛绂荤害涓?/p>
75
锝?/p>
150nm
銆?/p>
鎷寸郴鍚庡泭娉¤繘涓�姝ラ潬杩戦澏鑶滐紝
涓ゅ眰鑶滀箣闂寸殑璺濈绾︿负
5
锝?/p>
10nm
锛?/p>
姝よ繃绋嬫垨鐘舵�佸嵆鍥?/p>
娉＄殑閿氬畾銆?/p>
鍥婃场鐨勬嫶绯诲拰閿氬畾瀵逛繚璇佸泭娉′笌闈惰啘鐨勭壒寮傛�ц瀺鍚堛�?/p>
浠ュ強缁嗚優鍦ㄦ纭殑鏃堕棿鍜岀┖
闂村唴杩涜鑶滀笌铔嬬櫧璐ㄧ殑杞繍鍏锋湁閲嶈鎰忎箟锛?/p>
瀵圭淮鎸佸悇缁嗚優鍣ㄧ殑鐗瑰紓鎬т篃鍗佸垎鍏抽敭銆?/p>
鍥婃场浠庢嫶
绯荤姸鎬佽浆鍙樹负閿氬畾鐘舵�佺殑鍒嗗瓙鏈哄埗鐩墠杩樹笉娓呮銆?/p>
涓�绉嶅彲鑳芥槸鎷寸郴澶嶅悎鐗╁湪涓庡泭娉＄粨鍚堝悗鍙?/p>
鐢熷ぇ鐨勬瀯璞℃敼鍙橈紝鐗垫媺鍥婃场浣夸箣闈犺繎闈惰啘锛涘彟涓�绉嶅彲鑳芥槸
Sec1
锛?/p>
Munc18
铔嬬櫧涓?/p>
Rab
铔嬬櫧鏁?/p>
搴旂墿鍙婃嫶绯诲鍚堢墿鎴栧叾瀹冪粏鑳炶啘铔嬬櫧鐩镐簰浣滅敤锛屼績杩涘泭娉′粠鎷寸郴鐘舵�佽浆鍙樹负閿氬畾鐘舵�併�?/p>
1
锛?/p>
4
鍥婃场鐨勬縺娲?/p>