1
楂樻晥娑茬浉鑹茶氨鐭ヨ瘑鍒嗕韩
鎬昏堪
锛氭牴鎹牱鍝佸湪娴佸姩鐩稿拰鍥哄畾鐩镐腑鐨勫垎閰嶇郴鏁扮殑涓嶅悓灏嗙粍鍒嗗垎绂诲紑鏉ワ紝渚濇閫氳繃绱
妫�娴嬪櫒妫�娴嬶紝鍐嶉�氳繃鑹茶氨宸ヤ綔绔欒緭鍑鸿壊璋辨祦鍑烘洸绾匡紝渚濇嵁鑹茶氨娴佸嚭鏇茬嚎鎴戜滑鍙互寰楀埌锛氣�滆壊
璋卞嘲涓暟鈥斺�旂粍鍒嗘渶灏戜釜鏁帮紱淇濈暀鍊尖�斺�斿畾鎬у垎鏋愶紱闈㈢Н銆佸嘲楂樷�斺�斿畾閲忓垎鏋愶紱淇濈暀鍊笺�佸尯
鍩熷搴︹�斺�斿垽鏂壊璋辨煴鐨勫垎绂绘晥鑳斤紱涓ゅ嘲闂寸殑璺濈鈥斺�旇瘎浠峰浐瀹氱浉銆佹祦鍔ㄧ浉鐨勯�夋嫨鍙婇厤姣旀槸
鍚﹀悎閫傗�濈瓑淇℃伅銆?/p>
涓嬮潰浠庝互涓嬪嚑鏂归潰杩涜闃愯堪锛堝師鐞嗐�佽澶囥�佹敞鎰忎簨椤癸級锛?/p>
I
锛庢璁?/p>
涓�銆佹恫鐩歌壊璋辩悊璁哄彂灞曠畝鍐?/p>
1
銆佽壊璋辨硶鐨勮癁鐢?/p>
鑹茶氨娉曟渶鏃╂槸鐢变縿鍥芥鐗╁瀹惰尐缁寸壒
锛?/p>
Tswett
锛?/p>
鍦?/p>
1906
骞寸爺绌剁敤纰抽吀閽欏垎绂绘鐗╄壊绱犳椂
鍙戠幇鐨勶紝鑹茶氨娉?/p>
(Chromatography)
鍥犱箣寰楀悕銆傚悗鏉ュ湪姝ゅ熀纭�涓婂彂灞曞嚭绾歌壊璋辨硶銆佽杽灞傝壊璋辨硶銆?/p>
姘旂浉鑹茶氨娉曘�佹恫鐩歌壊璋辨硶銆?/p>
2
銆?/p>
HPLC
鐨勭壒鐐瑰拰浼樼偣
2.1 HPLC
鏈変互涓嬬壒鐐癸細
楂樺帇
-
鍘嬪姏鍙揪
150~300Kg/cm2
銆傝壊璋辨煴姣忕背闄嶅帇涓?/p>
75 Kg/cm2
浠ヤ笂銆?/p>
楂橀�?/p>
-
娴侀�熶负
0.1~10.0 ml/min
銆?/p>
楂樻晥
-
鍙揪
5000
濉旀澘姣忕背銆傚湪涓�鏍规煴涓悓鏃跺垎绂绘垚浠藉彲杈?/p>
100
绉嶃�?/p>
楂樼伒鏁忓害
-
绱妫�娴嬪櫒鐏垫晱搴﹀彲杈?/p>
0.01ng
銆傚悓鏃舵秷鑰楁牱鍝佸皯銆?/p>
2.2
HPLC
涓庣粡鍏告恫鐩歌壊璋辩浉姣旀湁浠ヤ笅
浼樼偣
锛?/p>
閫熷害蹇?/p>
-
閫氬父鍒嗘瀽涓�涓牱鍝佸湪
15~30 min
锛屾湁浜涙牱鍝佺敋鑷冲湪
5 min
鍐呭嵆鍙畬鎴愩�?/p>
鍒嗚鲸鐜囬珮
-
鍙�夋嫨鍥哄畾鐩稿拰娴佸姩鐩镐互杈惧埌鏈�浣冲垎绂绘晥鏋溿�?/p>
鐏垫晱搴﹂珮
-
绱妫�娴嬪櫒鍙揪
0.01ng
锛岃崸鍏夊拰鐢靛寲瀛︽娴嬪櫒鍙揪
0.1pg
銆?/p>
鏌卞瓙鍙弽澶嶄娇鐢?/p>
-
鐢ㄤ竴鏍硅壊璋辨煴鍙垎绂讳笉鍚岀殑鍖栧悎鐗┿�?/p>
鏍峰搧閲忓皯锛屽鏄撳洖鏀?/p>
-
鏍峰搧缁忚繃鑹茶氨鏌卞悗涓嶈鐮村潖锛屽彲浠ユ敹闆嗗崟涓�缁勫垎鎴栧仛鍒跺銆?/p>
鑹茶氨娉曠殑
鍒嗙鍘熺悊
鏄細婧朵簬娴佸姩鐩?/p>
(mobile phase)
涓殑鍚勭粍鍒嗙粡杩囧浐瀹氱浉鏃讹紝鐢变簬涓庡浐瀹氱浉
(stationary phase)
鍙戠敓浣滅敤锛堝惛闄勩�佸垎閰嶃�佺瀛愬惛寮曘�佹帓闃汇�佷翰鍜岋級鐨勫ぇ灏忋�佸己寮变笉鍚岋紝
鍦ㄥ浐瀹氱浉涓粸鐣欐椂闂翠笉鍚岋紝浠庤�屽厛鍚庝粠鍥哄畾鐩镐腑娴佸嚭銆傚張绉颁负鑹插眰娉曘�佸眰鏋愭硶銆?/p>
浜屻�佸熀鏈蹇靛拰鏈
1
锛庤壊璋卞浘鍜屽嘲鍙傛暟