鍝佷繚閮ㄥ疄楠屾搷浣滄寚瀵间功
缂?/p>
鍙?/p>
鐗?/p>
鏈?/p>
椤?/p>
鐮?/p>
3
鍒?/p>
瀹?/p>
瀹?/p>
鏍?/p>
鎵?/p>
鍑?/p>
涓婚
鍒嗘瀽瀹ゆ牱鍝佹娴嬫寚瀵间功
缂栧彿
DNA
闈炲彉鎬ц仛涓欑儻閰拌兒鍑濊兌鐢垫吵閾舵煋
1
锛?/p>
鐩殑锛氳鑼?/p>
閾舵煋妫�娴嬮潪鍙樻�ц仛涓欑儻閰拌兒鍑濊兌涓殑铔嬬櫧璐ㄦ垨
DNA
鐨勬祴瀹氭柟娉曚笌鎿嶄綔锛岀壒鍒?/p>
瀹氭湰瑙勭▼銆?/p>
2
锛庤寖鍥达細鏈绋嬮�傜敤浜庡凡鐭ヨ泲鐧借〃杈剧殑娴嬪畾銆?/p>
3
锛庤矗浠伙細瀹為獙瀹ゆ楠屼汉鍛樺瀹炴柦鏈绋嬭礋璐ｃ�?/p>
4
锛庡疄楠屽師鐞嗭細
鍦ㄧ⒈鎬ф潯浠朵笅锛岀敤鐢查啗灏嗚泲鐧藉甫涓婄殑纭濋吀閾讹紙閾剁瀛愶級杩樺師鎴愰噾灞為摱锛屼互浣?/p>
閾堕绮掓矇绉湪铔嬬櫧甯︿笂銆?/p>
鏌撹壊鐨勭▼搴︿笌铔嬬櫧涓殑涓�浜涚壒娈婄殑鍩哄洟鏈夊叧锛?/p>
涓嶅惈鎴栬�呭緢灏戝惈鍗婅儽
姘ㄩ吀娈嬪熀鐨勮泲鐧借川鏈夋椂鍊欏憟璐熸煋銆?/p>
5
锛庝华鍣ㄤ笌鍣ㄥ叿
绉绘恫鏋拰鏋ご銆佽劚鑹叉憞搴娿�?/p>
EP
绠°�?/p>
205ml
閿ュ舰鐡躲�佸鏂欑洅銆佺數娉充华銆佸瀭鐩寸數娉虫Ы鍙婂叾闄?/p>
浠躲�佺幓鐠冩澘
6
锛庣數娉宠瘯鍓?/p>
6.1
锛?/p>
30%
涓欑儻閰拌兒婧舵恫锛?/p>
涓欑儻閰拌兒锛?/p>
29g,N,N
鈥?/p>
-
浜氱敳鍩哄弻涓欑儻閰拌兒锛?/p>
1g,
鍔犲幓绂诲瓙姘村畾瀹硅嚦
100ml
锛岃浜庢
鑹茬摱鍐咃紝
4
鈩冨彲淇濆瓨涓や釜鏈堛�?/p>
6.2
锛庤繃纭吀閾?/p>
0.1g/ml
锛氱О鍙栬繃纭吀閾?/p>
1g
鍔犲幓绂诲瓙姘村�?/p>
10ml
銆?/p>
4
鈩冨彲淇濆瓨涓�鍛紝
-20
鈩冨彲淇?/p>
瀛樹竴涓湀銆?/p>
6.3
锛?/p>
TEMED(
鍥涚敳鍩轰箼鐑熀浜岃兒
)
锛氬拰
杩囩～閰搁摰涓�璧蜂綔涓轰氦鑱斿弽搴旂殑鍌寲鍓傘�傛槗鍚告疆锛?/p>
4
鈩冨皝
闂繚瀛樸�?/p>
6.4. 5*TBE
鐢垫吵缂撳啿娑?/p>
鍒嗗埆绉板彇
54
鍏?/p>
Tris,27.5
鍏嬬〖閰革紝
20ml0.5M/EDTA(pH=8.0)
瀹氬鑷?/p>
1L
6.5.
涓婃牱
Loading Buffer
鍒嗗埆鍑嗙‘绉伴噺婧撮厷钃濆拰浜岀敳鑻潚鍚?/p>
0.1
鍏嬶紝杞叆
50ml
瀹归噺鐡朵腑锛堥粡闄勫湪绉伴噺绾镐笂鐨勫皯閲?/p>
绮夋湯鍙敤绉绘恫鍣ㄨタ鍖虹敳閰拌兒閽熶钩瀹归噺鐡朵腑锛?/p>
锛屽啀浠嬪叆
1mlEDTA
锛屾渶鍚庣敤鐢查叞鑳哄畾瀹硅嚦
50ml
銆?/p>
7.
閾舵煋璇曞墏