Bac-to-Bac
鏉嗙姸鐥呮瘨琛ㄨ揪绯荤粺
璇曞墏鐩掑唴瀹圭墿
锛?/p>
Introduction
锛?/p>
Overview
锛?/p>
Bac-to-Bac
鏉嗙姸鐥呮瘨琛ㄨ揪绯荤粺鎻愪緵蹇�熸湁鏁堢殑鏂规硶浜х敓閲嶇粍鏉嗙姸鐥呮瘨銆傛鏂规硶鍩轰簬璁╁凡缁忚浆鍏ユ潌鐘剁梾鐨勮川绮掞紙鏉嗙矑锛?/p>
鐨勪綅鐐圭壒鎰忚浆搴у瓙鐨勮〃杈炬鐨勮川绮掑湪
Ecoli
涓墿澧炪�?/p>
Bac-to-Bac
鏉嗙姸鐥呮瘨琛ㄨ揪绯荤粺涓昏鍖呮嫭锛?/p>
*pFastBac
鎹愮尞璐ㄧ矑鐨勯�夋嫨锛屽畠瑕佽兘澶熶骇鐢熷寘鍚洰鐨勪綅鐐圭殑琛ㄨ揪缁撴瀯锛岃繖涓洰鐨勫熀鍥犵殑浜х敓琚潌鐘剁梾姣掔壒鎰忎綅鐐瑰惎鍔ㄥ瓙
鎺у埗銆?/p>
*
涓�涓?/p>
Ecoli
瀹夸富锛?/p>
DH10Bac
锛?/p>
鍖呭惈鏉嗙姸鐥呮瘨璐ㄧ矑
锛堟潌绮掞級
鍜岃緟鍔╄川绮掞紝
鍦ㄨ浆鏌?/p>
pFastBac
琛ㄨ揪缁撴瀯鍚庡彲浠ヤ骇鐢熼噸缁勬潌绮掋�?/p>
*
涓�涓帶鍒惰〃杈剧殑璐ㄧ矑锛屽寘鎷?/p>
Gus
鍜?/p>
/
鎴?/p>
CAT
鍩哄洜锛屼互渚垮湪鎰熸煋缁嗚優鍚庝骇鐢熼噸缁勬潌鐘剁梾姣掞紝琛ㄨ揪尾
-
钁¤悇绯栭吀閰愰叾鍜?/p>
/
鎴?/p>
姘湁绱犱箼閰拌浆绉婚叾銆?/p>
Bac-to-Bac
琛ㄨ揪绯荤粺鐨勪紭鐐?/p>
锛?/p>
浣跨敤杩欎釜绯荤粺浜х敓閲嶇粍鏉嗙姸鐥呮瘨杈冧紶缁熺殑鍚屾簮閲嶇粍鏈変互涓嬩紭鐐癸細
*
涓庝娇鐢ㄥ悓婧愰噸缁勪骇鐢熼噸缁勬潌鐘剁梾姣掓墍闇�鐨?/p>
4-6
鍛ㄧ浉姣旓紝閴村埆绾寲閲嶇粍鐥呮瘨灏戜簬涓ゅ懆
*
鍑忓皯浜嗕粠鏂戠偣绛涢�夐噸缁勭梾姣?/p>
DNA
鎵�鍖呭惈浜茬紭鍜岄潪閲嶇粍鐥呮瘨鐨勫嚑鐜?/p>
*
鍙互蹇�熷悓鏃惰繘琛屽ぇ閲忛噸缁勶紝閫傚悎琛ㄨ揪鍔熻兘鎬х爺绌剁殑铔嬬櫧
閫夋嫨
pFastBac
鑿屼綋锛?/p>
Vector
锛?/p>
锛?/p>
澶ч噺鐨?/p>
pFastBac
鑿屼綋閮介�備簬杩涜
Bac-to-Bac
琛ㄨ揪绯荤粺銆傞�夋嫨瀵逛簬浣犵殑闇�瑕佹渶鍚堥�傜殑鑿屼綋銆?/p>
Vector
鐗圭偣
鍙傝�?/p>
pFastBac
TM
1
楂樻按骞宠〃杈剧殑寮?/p>
AcMNPV
鑱?/p>
涔欑儻锛?/p>
PH
锛夊惎鍔ㄥ瓙
鐢ㄤ簬绠�鍗曞厠闅嗙殑澶ч噺鍏嬮殕浣?/p>
鐐?/p>
Anderon 1996
pFastBac
TM
HT
楂樻按骞宠〃杈剧殑鑱氫箼鐑惎鍔ㄥ瓙锛?/p>
N-
鏈鍚湁
6XHis
锛?/p>
鍙互鐢ㄦ潵
绾寲閲嶇粍铔嬬櫧锛屽苟鍙敤
TEV
铔嬬櫧閰跺垏鍘伙紱
鎻愪緵
3
涓槄璇绘
Polayes 1996
pFastBac
TM
Dual
涓や釜寮哄惎鍔ㄥ瓙锛?/p>
PH
鍜?/p>
p10
锛?
Harris
鍜?/p>
Polaye 1997