瀹為獙鍗?/p>
琛�绯栫殑娴嬪畾
琛�娑蹭腑鐨勮憽钀勭硸绉拌绯栥�傛甯镐汉琛�绯栨祿搴﹁緝鎭掑畾锛岀淮鎸佸湪
3
锛?/p>
9mmol
锛?/p>
L
锝?/p>
6
锛?/p>
1mmol
锛?/p>
L
涔嬮棿銆?/p>
琛�绯栨祿搴︾殑鐩稿鎭掑畾鏄満浣撹繘琛屾甯哥敓鐞嗘椿鍔ㄧ殑鍓嶆彁鏉′欢涔嬩竴锛?/p>
鏈夌潃鍙岄噸瀹為檯鎰忎箟锛?/p>
鍏朵竴锛岀淮鎸佺ǔ瀹氱殑鑳芥簮渚涚粰锛屾弧瓒虫満浣撳湪鍚勭鐢熺悊鐘舵�佷笅瀵硅兘閲忕殑闇�姹傘�傚叾浜岋紝淇濊瘉鏈轰綋
涓嶅洜杩涢鑷磋绯栨祿搴﹁繃楂橈紝瀵艰嚧绯栫殑涓㈠け銆?/p>
鍥犳锛岃绯栫殑娴嬪畾鏄复搴婄敓鍖栨楠屽疄楠屽鐨勫父瑙勬娴嬮」鐩�傝绯栨祴瀹氭寜鍏跺彂灞曡繃绋嬪強
鍙嶅簲鍘熺悊鐨勪笉鍚岋紝澶ц嚧鍒嗕笁绫伙細姘у寲杩樺師娉?/p>
锛岀缉鍚堟硶锛堜富瑕佹湁閭荤敳鑻兒娉曪級
锛岄叾娉曪紙涓昏
鏈夊繁绯栨縺閰舵硶鍜岃憽钀勭硸姘у寲閰舵硶锛?/p>
銆備笅杈逛粙缁嶄袱绉嶆柟娉曚緵閫夋嫨銆?/p>
涓�銆佽憽钀勭硸姘у寲閰舵硶
銆愮洰鐨勩�?/p>
1.
浜嗚В钁¤悇绯栨哀鍖栭叾娉曟祴瀹氳绯栫殑鍘熺悊锛岃兘杩涜琛�绯栨祴瀹氱殑鎿嶄綔銆?/p>
2.
鎺屾彙琛�绯栨祴瀹氱殑涓村簥鎰忎箟銆?/p>
銆愬師鐞嗐�?/p>
钁¤悇绯栨哀鍖栭叾
(glucose oxidase
锛?/p>
GOD)
鑳藉皢钁¤悇绯栨哀鍖栦负钁¤悇绯栭吀鍜岃繃姘у寲姘€�傚悗
鑰呭湪杩囨哀鍖栫墿閰?/p>
(peroxidase
锛?/p>
POD)
浣滅敤涓嬶紝
鍒嗚В涓烘按鍜屾哀鐨勫悓鏃跺皢鏃犺壊鐨?/p>
4-
姘ㄥ熀瀹夋浛姣?/p>
鏋椾笌閰氭哀鍖栫缉鍚堢敓鎴愮孩鑹茬殑閱岀被鍖栧悎鐗╋紝鍗?/p>
Trinder
鍙嶅簲銆傚叾棰滆壊鐨勬繁娴呭湪涓�瀹氳寖鍥村唴
涓庤憽钀勭硸娴撳害鎴愭姣旓紝鍦?/p>
505nm
娉㈤暱澶勬祴瀹氬惛鍏夊害锛屼笌鏍囧噯绠℃瘮杈冨彲璁＄畻鍑鸿绯栫殑娴撳害銆?/p>
鍙嶅簲寮忓涓嬶細
2H
2
O
2
+
4-姘ㄥ熀瀹夋浛姣旀灄
+
閰?
绾㈣壊閱岀被鍖栧悎鐗?
POD
2H
2
O
2H
2
O
2
O
2
钁¤悇绯?/p>
+
钁¤悇绯栭吀
+
GOD
+
銆愬櫒鏉愩�?/p>
璇曠銆佸惛绠°�佽瘯绠℃灦銆佹亽娓╂按娴寸銆佸垎鍏夊厜搴﹁
銆愯瘯鍓傘�?/p>
1. 0.1mol/L
纾烽吀鐩愮紦鍐叉恫锛?/p>
pH7.0
锛?/p>
绉板彇鏃犳按纾烽吀姘簩閽?/p>
8.67g
鍙婃棤姘寸７閰镐簩姘㈤捑
5.3g
婧朵簬
800ml
钂搁姘翠腑锛岀敤
1mol/L
姘㈡哀鍖栭挔锛堟垨
1mol/L
鐩愰吀锛夎皟鑺?/p>
pH
鑷?/p>
7.0
锛岀劧鍚庣敤钂搁姘寸█閲婅嚦
1L
銆?/p>
2.
閰惰瘯鍓?/p>
绉板彇杩囨哀鍖栫墿閰?/p>
1200U
锛岃憽钀勭硸姘у寲閰?/p>
1200U
锛?/p>
4-
姘ㄥ熀瀹夋浛姣旀灄
10mg
锛屽彔姘挔
100mg
锛?/p>
婧朵簬涓婅堪纾烽吀鐩愮紦鍐叉恫
80ml
涓紝鐢?/p>
1mol/L NaOH
璋?/p>
pH
鑷?/p>
7.0
锛屽姞纾烽吀缂撳啿娑茶嚦
100ml
銆?