涓浗绉戝闄?/p>
2002
骞寸爺绌剁敓鍏ュ璇曢銆婄敓鐗╁寲瀛︿笌鍒嗗瓙鐢熺墿瀛︺�?/p>
涓�銆佹槸闈為锛?/p>
15
棰橈紝姣忛
1
鍒嗭紝鍏?/p>
15
鍒嗐�傜瓟
"
鏄?/p>
"
鍐?/p>
"+"
锛岀瓟
"
闈?/p>
"
鍐?/p>
"- "
锛屽啓鍦ㄩ鍚庣殑锛堬級
涓�?/p>
1
锛庣淮鐢熺礌瀵逛汉浣撶殑鐢熼暱鍜屽仴搴锋槸蹇呴渶鐨勶紝浣嗕汉浣撲笉鑳藉悎鎴愮淮鐢熺礌銆?/p>
锛?/p>
锛?/p>
2
锛庤兘琚煇绉嶆尟濂嬪垎瀛愯瘑鍒紝骞朵笌鍏剁壒寮傚拰鍏变环缁撳悎鐨勫師瀛愶紝鍘熷瓙鍥㈠拰鍒嗗瓙锛岀О涓洪厤鍩恒�?/p>
锛?/p>
锛?/p>
3
锛庡綋涓嶅悓鍒嗗瓙澶у皬鐨勮泲鐧借川娣峰悎鐗╂祦缁忓嚌鑳舵煴灞傛瀽鏃讹紝灏忓垎瀛愮墿璐ㄥ洜浣撶Н灏忔渶鍏堣娲楄劚鍑?/p>
鏉ャ�?/p>
锛?/p>
锛?/p>
4
锛庨叾鐨勬渶閫?/p>
pH
涓庨叾鐨勭瓑鐢电偣鏄袱涓笉鍚岀殑姒傚康锛屼絾涓よ�呬箣闂存湁鐩稿叧鎬э紝涓や釜鏁板�奸�氬父姣?/p>
杈冩帴杩戞垨鐩稿悓銆?/p>
锛?/p>
锛?/p>
5
锛?/p>
瀵逛簬涓�涓叾鑰岃█锛?/p>
鍏惰繃娓℃�佺殑搴曠墿绫讳技鐗╀笌搴曠墿鐨勭墿鐩告瘮杈冿紝
鏄洿鏈夋晥鐨勭珵浜夋�ф姂鍒跺墏銆?/p>
锛?/p>
锛?/p>
6
锛?/p>
Km
鍊兼槸閰剁殑鐗插父鏁颁箣涓�锛?/p>
涓庨叾娴撳害銆?/p>
pH
鍊笺�?/p>
绂诲瓙寮哄害绛夋潯浠舵垨鍥犵礌鏃犲叧銆?/p>
锛?/p>
锛?/p>
7
锛庣７鑴傞叾
A
姘磋В鑴傜敓鎴愮７鑴傞吀銆?/p>
锛?/p>
锛?/p>
8
锛?/p>
NAD
涓嶈兘鐢辩粏鑳炴祮閫氳繃绾跨矑浣撳唴鑶滆繘鍏ョ嚎鏌嗕綋鍐咃紝
鑰?/p>
NADH
鑳藉湪閫氳繃绾跨矑浣撳唴鑶滃悗琚?/p>
姘у寲銆?/p>
锛?/p>
锛?/p>
9
锛庡闇夌礌鏄嚎绮掍綋
ATP
鍚堟垚閰剁殑鎶戝埗鍓傘�?/p>
锛?/p>
锛?/p>
10
锛庢牳鑻风７閰稿寲閰跺偓鍖栬吅鑻风殑纾烽吀鍖栵紝鐢熸垚鑵哄槍鍛ゅ拰鏍哥硸
-5-
纾烽吀銆?/p>
锛?/p>
锛?/p>
12
锛庤偪鐦?/p>
RNA
鐥呮瘨鐨勫鍒惰繃绋嬩负
RNA->DNA->RNA
銆?/p>
锛?/p>
锛?/p>
13
锛庤偩涓婅吅绱犺兘涓庣粏鑳炶啘涓婁笓涓�鍙椾綋缁撳悎锛岃繖绉嶆縺绱?/p>
鍙椾綋澶嶅悎鐗╄兘鐩存帴娲诲寲鐜寲
閰讹紝浣跨粏鑳?/p>
cAMP
娴撳害澧炲姞锛屽紩璧风骇鑱斿弽搴斻�?/p>
锛?/p>
锛?/p>
14
锛庣淮鐢熺礌
E
鏄竴绉嶆姉姘у寲鍓傦紝瀵圭嚎浣撹啘涓婄殑纾疯剛鏈夋姉鑷敱鐨勪綔鐢ㄣ�?/p>
锛?/p>
锛?/p>
15
锛庡悺鍝嗛啗銆佸悺鍝嗚兒鍜屽悺鍝嗛唶鐨勭７閰搁叝閮藉彲浠ヤ綔涓鸿浆姘ㄧ殑杈呴叾銆?/p>
锛?/p>
锛?/p>
浜屻�侀�夋嫨棰橈細
20
棰橈紝姣忛
1
鍒嗭紝鍏?/p>
20
鍒嗐�傝灏嗛�夋嫨绛旀鐨勫彿鐮佸～鍏ワ紙锛変腑銆?/p>
1
锛庝负绋冲畾鑳跺師涓夎偂铻烘棆缁撴瀯锛屼笁鑱斾綋鐨勬瘡涓変釜姘ㄥ熀閰哥殑浣嶇疆蹇呴』鏄細
锛?/p>
锛?/p>
鈶犱笝姘ㄩ吀锛涒憽璋锋皑閰革紱鈶㈢敇姘ㄩ吀
2
锛庡垎绂诲惈鑳′簩纭敭鐨勮偨娈靛彲浠ョ敤
锛?/p>
锛?/p>
鈶?/p>
SDS PAGE
鐢垫吵锛涒憽瀵硅绾跨數娉筹紱鈶㈢惣鑴傜硸鐢垫吵
3
锛庡紩璧风柉鐗涚梾锛堢墰娴风坏鑴戠梾锛夌殑鐥呭師浣撴槸锛?/p>
锛?/p>
锛?/p>
鈶犱竴绉?/p>
DNA
锛涒憽涓�绉?/p>
RNA
锛涒憿涓�绉嶈泲鐧借川锛涒懀涓�绉嶅绯?/p>
4
锛庤儼宀涚礌绛夋縺绱犵殑鍙椾綋浠ュ強涓婃垚鎴栬〃鐨敓闀垮洜瀛愮殑鍙椾綋閮芥槸涓�绉嶏細
锛?/p>
锛?/p>
鈶犳縺閰讹紱鈶¤劚姘㈤叾锛涒憿杞皑閰?/p>
5
锛庡湪閰剁殑鍙�嗘姂鍒跺墏涓紝涓嶅奖鍝嶉叾鐨勪簩绾у父鏁帮紙
Kcat/Km
锛夌殑鏄細
锛?/p>
锛?/p>
鈶犵珵浜夋�ф姂鍒跺墏锛涒憽闈炵珵浜夋�ф姂鍒跺墏锛涒憿鍙嶇珵浜夋�ф姂鍒跺墏锛涒懀閮戒笉鏄?/p>
6
锛庢墍璋?/p>
"
澶氶叾浣撶郴
"
鏄寚涓�涓唬璋㈣繃绋嬩腑鍑犱釜閰舵畻浜嗕竴涓弽搴旈摼浣撶郴锛屽閰朵綋绯讳腑鐨勯叾閫氬父
鍏锋湁浠ヤ笅鎬ц川銆?/p>
锛?/p>
锛?/p>
鈶?/p>
鍙槸鍦ㄥ姛鑳戒笂鐩镐簰鏈夎仈绯伙紝
鍦ㄧ粨鏋勪笂浜掍笉鐩稿叧锛?/p>
涓嶅瓨鍦ㄧ浉浜掍綔鐢紱
鈶?/p>
涓嶄粎鍦ㄥ姛鑳戒笂鐩镐簰
鏈夎仈绯伙紝鍦ㄧ粨鏋勪笂涔熸湁鐩镐簰鑱旂郴锛屽舰鎴愬鍚堜綋锛涒憿
涓婅堪涓ょ鎯呭喌閮藉瓨鍦ㄣ�?/p>
7
锛庢墍璋?/p>
"
浠ｈ阿鐗?/p>
"
鎸囩殑鏄笅闈㈠摢涓�鏉★紵
锛?/p>
锛?/p>
鈶?/p>
鐗规寚浠ｈ阿鍙嶅簲涓殑鍙嶅簲鐗╋紱鈶?/p>
鐗规寚浠ｈ阿鍙嶅簲涓殑浜х墿锛?/p>
鈶?/p>
鐗规寚浠ｈ阿鍙嶅簲涓殑涓棿鐗╋紱鈶?/p>
鎵�鏈変唬璋㈠弽搴斾腑鐨勫弽搴旂墿銆佷腑闂寸墿鍜屼骇鐗╅兘绉颁负浠ｈ阿鐗┿�?/p>
8
锛庢湁鐨勯叾瀛樺湪澶氱鍚屽伐閰跺舰寮忥紝杩欎簺鍚屽伐閰舵墍鍌寲鐨勫弽搴旓細
锛?/p>
锛?/p>
鈶?/p>
骞朵笉瀹屽叏鐩稿悓锛涒憽
瀹屽叏鐩稿悓锛岃�屼笖鍙嶅簲鐨勫钩琛″父鏁颁篃鐩稿悓锛?/p>
鈶?/p>
瀹屽叏鐩稿悓锛屼絾鐢变簬姣忕鍚屽伐閰舵椿鎬т笉鍚岋紝鍙嶅簲骞宠 甯告暟鍙互涓嶇浉鍚屻�?/p>