鐚粰鍒濆鑰咃細澶х鏁欎綘鐬椂杞煋蹇紝鍑嗭紝鐙?/p>
涓�鑸儏鍐典笅锛岀灛鏃惰浆鏌撴槸灏?/p>
DNA
瀵煎叆鐪熸牳缁嗚優鐨勬柟寮忎箣涓�銆傚湪鐬椂杞煋涓紝閲嶇粍
DNA
瀵煎叆鎰熸煋鎬у己鐨勭粏鑳炵郴浠ヨ幏寰楃洰鐨勫熀鍥犳殏鏃朵絾楂樻按骞崇殑琛ㄨ揪銆?/p>
杞煋鐨?/p>
DNA
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鏀惰幏杞煋鐨勭粏鑳烇紝骞跺婧惰В浜х墿涓洰鐨勫熀鍥犵殑琛ㄨ揪杩涜妫�娴嬨�?/p>
濡傛灉杩囪〃杈惧熀鍥犵敤璐ㄧ矑锛?/p>
濡傛灉鏁查檷鐢ㄧ殑鏄?/p>
siRNA(
鍦?/p>
microRNA
瀹為獙涓紝
杩囪〃杈剧敤鐨?/p>
鏄?/p>
mimics
锛屾暡闄嶇敤鐨勬槸
inhibitor)
銆?/p>
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gain and loss
瀹為獙锛?/p>
锛岃瀵熶笅娓搁澏鍩哄洜
鐨勮〃杈炬儏鍐碉紝鎴栬�呯粏鑳炶〃鍨嬶紙
phenotype
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绛夈�傞偅璇ユ�庝箞鍋氬憿
01
瀹為獙鍓嶅噯澶?/p>
lipofectamine2000
锛堣浆鏌撹瘯鍓傦級
锛堥伩鍏夛級
锛?/p>
Opti-MEMI
鍑忚娓呭煿鍏诲熀锛堥伩鍏夛級
锛岀洰鐨?/p>
鍩哄洜鐨勮川绮掓垨鑰?/p>
siRNA
锛堜互
siRNA
涓轰緥锛?/p>
锛岀粏鑳烇紝鍏瓟鏉匡紝鍩瑰吇鍩猴紝浠ュ強涓�浜涚粏鑳炲疄楠?/p>
鎵�闇�鐨勬櫘閫氱墿鍝併�?/p>
02
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