鐚粰鍒濆鑰咃細澶х鏁欎綘鐬椂杞煋蹇紝鍑嗭紝鐙?/p>
涓�鑸儏鍐典笅锛?/p>
鐬椂杞煋鏄皢
DNA
瀵煎叆鐪熸牳缁嗚優鐨勬柟寮?/p>
涔嬩竴銆傚湪鐬椂杞煋涓紝閲嶇粍
DNA
瀵煎叆鎰熸煋鎬у己鐨勭粏鑳炵郴浠?/p>
鑾峰緱鐩殑鍩哄洜鏆傛椂浣嗛珮姘村钩鐨勮〃杈俱�?/p>
杞煋鐨?/p>
DNA
涓嶅繀鏁村悎鍒板涓绘煋鑹蹭綋锛?/p>
鍙湪姣旂ǔ瀹氳浆鏌撹緝鐭?/p>
鏃堕棿鍐咃紙鏈�澶氫竴鍛ㄥ乏鍙筹級鏀惰幏杞煋鐨勭粏鑳烇紝骞跺婧惰В浜х墿涓?/p>
鐩殑鍩哄洜鐨勮〃杈捐繘琛屾娴嬨�?/p>
濡傛灉杩囪〃杈惧熀鍥犵敤璐ㄧ矑锛屽鏋滄暡闄嶇敤鐨勬槸
siRNA(
鍦?/p>
microRNA
瀹為獙涓紝杩囪〃杈剧敤鐨勬槸
mimics
锛屾暡闄嶇敤鐨勬槸
inhibitor)
銆?/p>
閫氳繃鐬椂杞煋锛屽皢鐩殑鍩哄洜鏁查檷鎴栬�呰繃琛ㄨ揪锛?/p>
gain and loss
瀹為獙锛?/p>
锛岃瀵熶笅娓搁澏鍩哄洜鐨勮〃杈炬儏鍐碉紝鎴栬�呯粏鑳炶〃鍨?/p>
锛?/p>
phenotype
锛夌殑鍙樺寲锛屼緥濡傚噵浜★紝鍛ㄦ湡锛屽娈栵紝渚佃锛岃浆
绉荤瓑銆傞偅璇ユ�庝箞鍋氬憿
01
瀹為獙鍓嶅噯澶?/p>
lipofectamine2000
锛堣浆鏌撹瘯鍓傦級
锛堥伩鍏夛級
锛?/p>
Opti-MEMI
鍑忚娓?/p>
鍩瑰吇鍩?/p>
锛堥伩鍏夛級
锛?/p>
鐩殑鍩哄洜鐨勮川绮掓垨鑰?/p>
siRNA
锛堜互
siRNA
涓?/p>
渚嬶級
锛岀粏鑳烇紝鍏瓟鏉匡紝鍩瑰吇鍩猴紝浠ュ強涓�浜涚粏鑳炲疄楠屾墍闇�鐨勬櫘
閫氱墿鍝併�?/p>
02