骞冲窛楂樹笁骞寸骇绗竴娆℃湀鑰冪敓鐗╄瘯鍗?/p>
绗?/p>
1
椤?/p>
鍏?/p>
7
椤?/p>
骞冲窛涓楂樹笁骞寸骇绗竴娆℃湀鑰冪敓鐗╄瘯鍗?/p>
2013-8-28
涓�銆侀�夋嫨棰?/p>
(
姣忓皬棰?/p>
1
鍒嗭紝鍏?/p>
40
鍒嗐�傛瘡灏忛鍙湁涓�涓纭瓟妗堛�?/p>
)
1
銆侀檺鍒堕叾鏄竴缇ゆ牳閰稿垏鍓查叾锛?/p>
鍙鲸璇嗗苟鍒囧壊
DNA
鍒嗗瓙涓婄壒瀹氱殑鏍歌嫹閰哥⒈鍩哄簭鍒椼�?/p>
涓嬪浘涓?/p>
鍥涚闄愬埗閰?/p>
BamHI
锛?/p>
EcoRI
锛?/p>
HindIII
浠ュ強
Bg
l
II
鐨勮鲸璇嗗簭鍒椼�傜澶磋〃绀烘瘡涓�绉嶉檺鍒堕叾鐨勭壒
瀹氬垏鍓查儴浣嶏紝鍏朵腑鍝袱绉嶉檺鍒堕叾鎵�鍒囧壊鍑烘潵鐨?/p>
DNA
鐗囨鏈鍙互浜掕ˉ鐐煎悎锛熷叾姝ｇ‘鐨勬湯
绔簰琛ュ簭鍒椾负浣曪紵
BamHI
EcoRI
HindIII
Bg
l
II
鈫?/p>
鈫?/p>
鈫?/p>
鈫?/p>
鈥?/p>
GGATCC
鈥?/p>
鈥?/p>
GAATTC
鈥?/p>
鈥?/p>
AACCTT
鈥?/p>
鈥?/p>
AGA
TCT
鈥?/p>
鈥?/p>
CCTAGG
鈥?/p>
鈥?/p>
CTTAAG
鈥?/p>
鈥?/p>
TTGGAA
鈥?/p>
鈥?/p>
TCTAGA
鈥?/p>
鈫?/p>
鈫?/p>
鈫?/p>
鈫?/p>
A
锛?/p>
BamHI
鍜?/p>
EcoRI
锛涙湯绔簰琛ュ簭鍒椻�?/p>
AA
TT
鈥?/p>
B
锛?/p>
BamHI
鍜?/p>
HindIII
锛涙湯绔簰琛ュ簭鍒椻�?/p>
GA
TC
鈥?/p>
C
锛?/p>
BamHI
鍜?/p>
Bg
l
II
锛涙湯绔簰琛ュ簭鍒椻�?/p>
GATC
鈥?/p>
D
锛?/p>
EcoRI
鍜?/p>
HindIII
锛涙湯绔簰琛ュ簭鍒椻�?/p>
AA
TT
鈥?/p>
2
銆佷笅鍒楁墍绀虹殑榛忔�ф湯绔槸鐢卞嚑绉嶉檺鍒舵�у唴鍒囬叾浣滅敤浜х敓
A
锛?/p>
1
绉?/p>
B
锛?/p>
2
绉?/p>
C
锛?/p>
3
绉?/p>
D
锛?/p>
4
绉?/p>
3
銆?/p>
鈥?/p>
X
鍩哄洜鈥濇槸
DNA
鍒嗗瓙涓婁竴涓湁閬椾紶鏁堝簲鐨勭墖娈碉紝鑻ヨ鐢?/p>
PCR
鎶�鏈壒寮傛�у湴鎷疯礉鈥?/p>
X
鍩哄洜鈥?/p>
锛岄渶鍦?/p>
PCR
鍙嶅簲涓姞鍏ヤ袱绉嶅紩鐗╋紝涓ょ寮曠墿鍙婂叾涓庢ā鏉块摼鐨勭粨鍚堜綅缃鍥?/p>
1
鎵�绀恒�?/p>
缁?/p>
4
杞惊鐜悗浜х墿涓湁浜旂涓嶅悓鐨?/p>
DNA
鍒嗗瓙锛屽鍥?/p>
2
鎵�绀猴紝鍏朵腑绗懁绉?/p>
DNA
鍒嗗瓙鏈夊嚑
涓?/p>
A
锛?/p>
2
B
锛?/p>
4
C
锛?/p>
6
D
锛?/p>
8
4
銆佷笅鍥炬墍绀猴紝绉戠爺浜哄憳鍚堟垚浜嗕竴娈靛惈
15
N
鐨勭壒瀹氱殑
DNA
鐗囨銆傜敤瀹冧綔涓烘帰閽堬紝鍒╃敤纰卞熀
浜掕ˉ閰嶅鍘熷垯锛屽彲妫�娴嬪緟娴嬫爣鏈腑鐨勯仐浼犱俊鎭�傝繖椤规妧鏈洰鍓嶄笉鍙簲鐢ㄥ埌涓嬪垪鍝釜鏂归潰锛?/p>
A
锛庤倽鐐庣瓑鐤剧梾璇婃柇
B
锛庨仐浼犵梾鐨勪骇鍓嶈瘖鏂?/p>
C
锛庝慨澶嶆湁缂洪櫡鐨勫熀鍥?/p>
D
锛庢娴嬮ギ姘翠腑鐥呮瘨
5
銆佺敤
DNA
杩炴帴閰舵妸琚檺鍒堕叾
I(
璇嗗埆搴忓垪鍜屽垏鐐规槸鈥?/p>
鈫?
GA
TC
鈥?/p>
)
鍒囧壊杩囩殑杩愯浇浣撳拰琚檺鍒?/p>
閰?/p>
II(
璇嗗埆搴忓垪鍜屽垏鐐规槸鈥?/p>
G
鈫?
GA
TCC
鈥?/p>
)
鍒囧壊杩囩殑鐩殑鍩哄洜杩炴帴璧锋潵鍚庯紝璇ラ噸缁?/p>
DNA
鍒嗗瓙
鑳藉鍐嶈闄愬埗閰?/p>
II
鍒囧壊寮�鐨勬鐜囨槸
A
锛?/p>
1/2
B
锛?/p>
7/16
C
锛?/p>
1/16
D
锛?/p>
1/4
6
銆?/p>
涓�瀵圭瓑浣嶅熀鍥犵粡鏌愮闄愬埗鎬у唴鍒囬叾鍒囧壊鍚庡舰鎴愮殑
DNA
鐗囨闀垮害瀛樺湪宸紓锛?/p>
鍑濊兌鐢垫吵鍒?/p>
绂婚叾鍒囧悗鐨?/p>
DNA
鐗囨锛屼笌鎺㈤拡鏉備氦鍚庡彲鏄剧ず鍑轰笉鍚岀殑甯﹁氨銆傛牴鎹鐗规�э紝灏辫兘灏嗗畠浣滀负
鏍囪瀹氫綅鍦ㄥ熀鍥犵粍鐨勬煇涓�浣嶇疆涓娿�?/p>
鐜版湁涓�瀵瑰か濡囩敓
浜嗗洓涓効濂筹紝鍏朵腑
1
鍙锋�х姸鐗规畩
(
濡傚彸鍥?/p>
)
锛岀敱姝ゅ彲
鎺ㄧ煡杩欏洓涓効濂崇殑鍩哄洜鍨?/p>
(
鐢?/p>
D
銆?/p>
d
琛ㄧず
)
姝ｇ‘鐨勬槸
A
锛?/p>
1
鍙蜂负
X
d
X
d
B
锛?/p>
2
鍙蜂负
X
D
Y
C
锛?/p>
3
鍙蜂负
X
D
X
d
D
锛?/p>
4
鍙蜂负
DD
7
銆佺瀛﹀宸茶兘杩愮敤鍩哄洜宸ョ▼鎶�鏈紝璁╃緤鍚堟垚骞剁敱涔宠吅鍒嗘硨鎶椾綋锛屼笅鍒楃浉鍏冲彊杩颁腑姝ｇ‘鐨?/p>
鏄?/p>
鈶犺鎶�鏈皢瀵艰嚧瀹氬悜鍙樺紓
鈶?/p>
DNA
杩炴帴閰舵妸鐩殑鍩哄洜涓庤繍杞戒綋榛忔�ф湯绔殑纰卞熀瀵硅繛
鎺ヨ捣鏉?/p>
鈶㈣泲鐧借川涓殑姘ㄥ熀閰稿簭鍒楀彲涓哄悎鎴愮洰鐨勫熀鍥犳彁渚涜祫鏂?/p>
鈶ｅ彈绮惧嵉鏄悊鎯崇殑鍙椾綋
A
锛庘憼鈶♀憿鈶?/p>
B
锛庘憼鈶⑩懀
C
锛庘憽鈶⑩懀
D
锛庘憼鈶♀懀
8
銆?/p>
鍦熷￥鍐滄潌鑿屾槸鍩哄洜宸ョ▼涓皢鐩殑鍩哄洜瀵煎叆妞嶇墿缁嗚優鏃跺父鐢ㄧ殑涓棿
濯掍粙銆傚畠鍚湁涓�涓ぇ鍨嬬殑
Ti
璐ㄧ矑
(
濡傚浘鎵�绀?/p>
)
锛屽湪渚垫煋妞嶇墿缁嗚優鐨勮繃
绋嬩腑锛?/p>
鍏朵腑鐨?/p>
T-DNA
鐗囨杞叆妞嶇墿鐨勫熀鍥犵粍銆?/p>
鑻ユ兂鐢ㄥ熀鍥犲伐绋嬪苟閫?/p>
杩囧湡澹ゅ啘鏉嗚弻鍚戞煇绉嶆鐗╀腑瀵煎叆鎶楁棻鍩哄洜銆備互涓嬪垎鏋愪笉鍚堢悊鐨勬槸
A
锛庤嫢鐢?/p>
Ti
璐ㄧ矑浣滀负鎶楁棻鍩哄洜鐨勮浇浣擄紝鐩殑鍩哄洜鐨勬彃鍏ヤ綅缃簲璇ュ湪
T-DNA
鐗囨鍐咃紝
涓旇淇濊瘉澶嶅埗璧峰鐐瑰拰鐢ㄤ簬杞Щ
T-DNA
鐨勫熀鍥犵墖娈典笉琚牬鍧?/p>
绾悎浣?/p>
绾悎浣?/p>
鏉傚悎浣?/p>