閫嗚浆褰?/p>
PCR
RT-PCR
涓哄弽杞綍
RCR
锛?/p>
reverse
transcription
PCR
锛?/p>
鍜屽疄鏃?/p>
PCR
锛?/p>
real
time
PCR
锛?/p>
鍏卞悓鐨勭缉鍐欍�傞�嗚浆褰?/p>
PCR
锛屾垨鑰呯О鍙嶈浆褰?/p>
PCR(reverse transcription-PCR,
RT-PCR)
锛屾槸鑱氬悎閰堕摼寮忓弽搴?/p>
(PCR)
鐨勪竴绉嶅箍娉涘簲鐢ㄧ殑鍙樺舰銆傚湪
RT-PCR
涓紝涓�鏉?/p>
RNA
閾捐閫嗚浆褰曟垚涓轰簰琛?/p>
DNA
锛屽啀浠ユ涓烘ā鏉块�氳繃
PCR
杩涜
DNA
鎵╁銆?/p>
閫嗚浆褰?/p>
PCR
鐢变竴鏉?/p>
RNA
鍗曢摼杞綍涓轰簰琛?/p>
DNA
(cDNA)
绉颁綔
鈥?/p>
閫嗚浆褰?/p>
鈥?/p>
锛岀敱渚濊禆
RNA
鐨?/p>
DNA
鑱氬悎閰?/p>
锛堥�嗚浆褰曢叾锛?/p>
鏉ュ畬鎴愩�?/p>
闅忓悗锛?/p>
DNA
鐨勫彟涓�鏉￠摼閫氳繃鑴辨哀鏍歌嫹閰稿紩鐗╁拰渚濊禆
RNA
鐨?/p>
DNA
鑱氬悎閰跺畬鎴愶紝闅忔瘡涓惊鐜�嶅锛屽嵆閫氬父鐨?/p>
PCR
銆傚師鍏堢殑
RNA
妯℃澘琚?/p>
RNA
閰?/p>
H
闄嶈В锛岀暀涓嬩簰琛?/p>
DNA
銆?/p>
RT-PCR
鐨勬寚鏁版墿澧炴槸涓�绉嶅緢鐏垫晱鐨勬妧鏈紝
鍙互妫�娴嬪緢浣庢嫹璐濇暟鐨?/p>
RNA
銆?/p>
RT-PCR
骞挎硾搴旂敤浜庨仐浼犵梾鐨勮瘖鏂紝骞朵笖鍙互鐢ㄤ簬瀹氶噺鐩戞祴鏌愮
RNA
鐨勫惈閲忋�?/p>
锛堟娴嬪熀鍥犺〃杈?/p>
鐨勬柟娉曪紝鍙傝
Northern Blot
娉曘�?/p>
锛?/p>
RT-PCR
鏈夋椂鍊欎篃浼氭寚浠ｅ疄鏃?/p>
PCR(real-time
PCR)
銆備负浜嗕笌閫嗚浆褰?/p>
PCR
鐩稿尯鍒紝
閫氬父琚啓浣?/p>
鈥?/p>
瀹氶噺
PCR鈥?quantitative PCR)
鎴栬�?/p>
RTQ-PCR(real-time quantitative PCR)
銆?/p>
瀹炴椂
PCR
瀹炴椂
PCR(real-time PCR)
锛屽睘浜庡畾閲?/p>
PCR
锛?/p>
Q-PCR
锛夌殑涓�绉嶏紝浠ヤ竴瀹氭椂闂村唴
DNA
鐨勫骞呴噺涓哄熀纭�杩涜
DNA
鐨勫畾閲忓垎鏋愩�?/p>
real time PCR
鐨勫畾閲忎娇鐢ㄨ悿鍏夎壊绱狅紝鐩墠鏈変簩绉嶆柟娉曘�備竴绉嶆槸鍦?/p>
ds DNA
涓彃鍏?/p>
鐗瑰紓鐨勮悿鍏夎壊绱狅紱鍙︿竴绉嶄娇鐢ㄤ竴绉嶈兘涓庡骞?/p>
DNA
搴忓垪涓壒瀹氬鏍搁吀搴忓垪鐩哥粨鍚堢殑涓�
绉嶈悿鍏夋帰閽堬紙
probe
锛?/p>
銆?/p>
real
time
PCR
涓?/p>
reverse
transcription
PCR
鐩哥粨鍚堬紝鑳界敤寰噺鐨?/p>
RNA
鏉ユ壘鍑虹壒
瀹氭椂闂淬�佺粏鑳炪�佺粍缁囧唴鐨勭壒鍒〃杈剧殑閬椾紶鍩哄洜銆傝繖涓ょ
RT PCR
鐨勭粍鍚堝張琚О涔嬩负
鈥?/p>
瀹?/p>
閲?/p>
RT-PCR
锛?/p>
quantitative RT-PCR
锛?/p>
鈥?nbsp;
RT-PCR
鎶�鏈浉鍏宠瘯鍓?/p>
oligo:
澶氳仛浣擄紝鐩稿綋浜?/p>
mRNA
寮曠墿
AMV
锛?/p>
M-MLV
锛?/p>
锛氶�嗚浆褰曢叾
dNTP
锛氳劚姘ф牳鑻烽吀
RNase
锛?/p>
RNA
閰舵姂鍒跺墏
PCR Buffer
锛?/p>
RT-PCR
缂撳啿娑?/p>
MgCl2
锛?/p>
2
浠烽晛绂诲瓙