鍖椾含鍚夌編鐢熺墿鎶�鏈湁闄愬叕鍙?/p>
ACK
绾㈢粏鑳炶瑙ｆ恫
(ACK Lysis Buffer)
浜у搧绠�浠嬶細
鍦ㄧ敓鐗╃鐮旈鍩燂紝缁忓父闇�瑕佸幓闄ょ孩缁嗚優锛屽幓闄ょ孩缁嗚優鐨勬柟娉曟湁澶氱锛屽
ACK
Lysis
Buffer
銆?/p>
Tris-
姘寲閾电粏鑳炶瑙ｆ恫銆?/p>
Gey
锛?
s Lysis Buffer
銆?/p>
ACK
绾㈢粏鑳炶瑙ｆ恫
(Red Blood Cell Lysis
Buffer)
涔熺О
ACK Lysis Buffer
锛?/p>
鏄竴绉嶄粠浜恒�?/p>
榧犳垨鍏朵粬鍝轰钩鍔ㄧ墿绛変綋鍐呯殑缁勭粐鏍峰搧鎴栬娑蹭腑
瑁傝В骞跺幓闄ゆ棤鏍哥孩缁嗚優鐨勬憾娑诧紝鍏朵富瑕佹湁鏁堟垚鍒嗕负
NH
4
CI
銆?/p>
Jimei ACK Lysis Buffer
缁忚繃浼樺寲閰嶆柟锛屽湪瑁傝В鏃犳牳绾㈢粏鑳炵殑鍚屾椂鍑犱箮涓嶆崯浼ゆ穻宸寸粏鑳?/p>
(Lymphocyte)
鎴栧叾瀹冩湁缁嗚優鏍哥殑缁嗚優銆傚浜庤瑙ｃ�佸幓闄ゆ湁缁嗚優鏍哥孩缁嗚優锛屼緥濡傞笩鎴栫绫荤殑绾?/p>
缁嗚優锛?/p>
鏁堟灉涓嶄匠锛?/p>
瑁傝В绫讳技缁嗚優鏃讹紝
涓嶅缓璁噰鐢ㄣ�?/p>
鏈瑙ｆ恫缁忚繃婊ら櫎鑿岋紝
缁忚繃
ACK Lysis Buffe
澶勭悊杩囩殑琛�娑叉垨缁勭粐缁嗚優鏍峰搧鍙互鐢ㄤ簬鍚庣画鐨勭粏鑳炲煿鍏汇�?/p>
缁嗚優铻嶅悎浠ュ強鏍搁吀鎴栬泲鐧界殑鎻愬彇鍙?/p>
鍚勭甯歌鐨勫垎鏋愬拰妫�娴嬨�?/p>
浜у搧缁勬垚锛?/p>
鑷鏉愭枡锛?/p>
1
銆?/p>
鑳拌泲鐧介叾
2
銆?/p>
绂诲績鏈?/p>
3
銆?/p>
PBS
銆?/p>
HBSS
銆佺敓鐞嗙洂姘存垨琛�娓呭煿鍏绘恫
鎿嶄綔姝ラ
锛堜粎渚涘弬鑰冿級
锛?/p>
锛堜竴锛夌粍缁囩粏鑳炴牱鏈殑甯歌鎿嶄綔
1
銆?/p>
鍒跺缁嗚優鎮恫锛氭柊椴滅粍缁囩粡杩囪儼铔嬬櫧閰舵垨鑳跺師閰剁瓑娑堝寲澶勭悊锛岄�氳繃閫傚綋鏂规硶鍒?
澶囨垚缁嗚優鎮恫锛岀蹇冨純涓婃竻銆?/p>
2
銆?/p>
瑁傝В锛氬姞鍏?/p>
3~5
鍊嶇粏鑳炴矇娣�浣撶Н鐨?/p>
ACK
Lysis
Buffer
锛岃交鏌斿惞鎵撴贩鍖�锛岃瑙?
1~2min
銆傛湰鎿嶄綔姝ラ鍦?/p>
4
銆?
C
鏉′欢涓嬫搷浣滄洿浣筹紝浜﹀彲鍦ㄥ娓╀笅鎿嶄綔銆?/p>
3
銆?/p>
绂诲績锛?/p>
4
銆?
C
锛?/p>
400~500g
绂诲績
5
min
锛屽純绾㈣壊涓婃竻銆傚鏃犱綆娓╃蹇冩満锛屾湰姝ラ浜?
鍙湪瀹ゆ俯涓嬫搷浣溿�?/p>
4
銆?/p>
濡傛灉鍙戠幇绾㈢粏鑳炶瑙ｄ笉瀹屽叏锛屽彲浠ラ噸澶嶄笂杩版楠?/p>
2
鍜屾楠?/p>
3
鍚勪竴娆°�?/p>
5
銆?/p>
娲楁钉锛氭牴妞愬疄楠岃姹傚姞鍏ラ�傞噺
PBS
銆?/p>
HBSS
銆佺敓鐞嗙洂姘存垨鏃犺娓呭煿鍏绘恫锛岃交鏌?
娣峰寑閲嶆偓娌夋穩銆?/p>
4
銆?
C
锛?/p>
400~500g
绂诲績
2~3
min
锛屽純涓婃竻锛岃绂诲績姝ラ浜﹀彲鍦ㄥ
娓╀笅鎿嶄綔銆傛墍鍔犲叆鐨?/p>
PBS
銆?/p>
HBSS
銆佺敓鐞嗙洂姘存垨鏃犺娓呭煿鍏绘恫鐨勯噺涓�鑸簲澶т簬
缁嗚優娌夋穩浣撶Н鐨?/p>
5
鍊嶄互涓娿�?/p>
6
銆?/p>
濡傛湁蹇呰锛岄噸澶嶄笂杩版楠?/p>
5
涓�娆★紝鍏辨礂娑?/p>
1~2
娆°�?/p>
7
銆?/p>
鏍规嵁瀹為獙闇�瑕佺敤閫傚綋婧舵恫閲嶆偓缁嗚優娌夋穩锛岃繘琛岃鏁般�佸煿鍏荤瓑鍚庣画瀹為獙銆?/p>
锛堜簩锛夌粍缁囩粏鑳炴牱鏈殑蹇�熸搷浣?/p>
锛堟棤闇�娲楁钉锛?/p>
1
銆佸埗澶囩粏鑳炴偓娑诧細鏂伴矞缁勭粐缁忚繃鑳拌泲鐧介叾绛夋秷鍖栧鐞嗭紝閫氳繃閫傚綋鏂规硶鍒跺鎴愮粏鑳?
鎮恫锛岀蹇冨純涓婃竻銆?/p>
缂栧彿
鍚嶇О
JC2145
JC2146
storage
ACK
绾㈢粏鑳炶瑙ｆ恫
(ACK Lysis Buffer)
100
ml
500
ml
4
銆?
C
浣跨敤璇存槑涔?/p>
1
浠?/p>