鍐诲瓨鍓嶅悗楠ㄩ珦闂村厖璐ㄥ共缁嗚優鐨勭敓鐗╁鐗规�у拰鏀寔閫?
琛�鐨勭爺绌?/p>
浣滆�咃細璧垫櫤鍒氾紝鍞愭檽鐞硷紝榛庣含鏄庯紝娓告吵锛岄偣钀?/p>
銆愭憳瑕併�?/p>
涓轰簡浜嗚В鍐诲瓨澶嶈嫃杩囩▼瀵归楂撻棿鍏呰川骞茬粏鑳?/p>
(mesenchymal
stem cell
锛?/p>
MSC)
鐢熺墿瀛︾壒鎬у拰鏀寔閫犺鑳藉姏鐨勫奖鍝嶏紝閲囩敤甯歌鏂规硶鍒嗙鍩瑰吇
楠ㄩ珦
MSC
锛屽皢浼犱唬鍚庣殑缁嗚優鐢ㄥ惈
10
锛呬簩鐢蹭簹鐮溿�?/p>
40
锛呰儙鐗涜娓呯殑
IMDM
缁嗚優鍐诲瓨
娑蹭繚瀛樺湪
-
196鈩冩恫姘腑锛岃瀵熺煭鏈燂紙
4
鍛級鍜屼腑鏈燂紙
9-15
鏈堬級澶嶈嫃鍚?/p>
MSC
鐨勬椿
鎬с�佸厤鐤〃鍨嬨�佸鍚戝垎鍖栬兘鍔涘拰鏀寔閫犺鐨勮兘鍔涳紝骞跺悓鍐诲瓨鍓嶇殑
MSC
杩涜姣?/p>
杈冦�?/p>
缁撴灉琛ㄦ槑锛氫腑鐭湡鍐诲瓨鍚庣殑
MSC
鐨勭粏鑳炴椿鎬у垎鍒负
(90
澹?/p>
3.75)
锛呭拰
(93
澹?/p>
2.51)
锛咃紱鍐诲瓨鍚庣粏鑳炵殑澧炴畺鑳藉姏銆佸厤鐤〃鍨嬨�佷綋澶栧垎鍖栦负鑴傝偑鍜岄缁嗚優鐨?/p>
鑳藉姏銆佹敮鎸侀泦钀斤紙
CFU-GM
锛?/p>
CFU-E
锛?/p>
CFU-GEMM
锛夌殑鐢熼暱浣滅敤鍜屽喕瀛樺墠
MSC
鐩镐技銆?/p>
缁撹锛氶楂?/p>
MSC
鍦ㄦ恫姘腑鐭湡鍜屼腑鏈熶繚瀛樺悗锛岀粏鑳炴椿鎬х暐鏈変笅闄嶏紝浣嗘槸骞朵笉褰?/p>
鍝?/p>
MSC
鐨勫娈栥�佸垎鍖栧拰鏀寔閫犺鑳藉姏銆?/p>
銆愬叧閿瘝銆?/p>
鏀寔閫犺
Biological Characteristics and
Ability of Supporting Hematopoiesis of Bone Marrow Mesenchymal
Stem Cells Pre-and Post-Cryop-reservation
Abstract This
study was aimed to observe the biological characteristics of
cryopreserved bone marrow mesenchymal stem cell (MSC) and to examine
their abilities to support in vitro hematopoiesis.Bone marrow MSC
were cryopreserved in -
196鈩?nbsp;liquid nitrogen for 4 weeks (short term)
and 9-15 months (medium term) with IMDM containing 10% DMSO
锛?/p>
40%
fetal calf serum as cryoprotectant.The viability
锛?/p>
proliferation
锛?/p>
immunophenotype
锛?/p>
in vitro differentiation and ability of supporting
hematopoiesis of thawed MSC were investigated and compared with these
of pre-cryopreserved MSC.The results showed that the cell viability
were (93
澹?/p>
2.51)
锛?/p>
and (90
澹?/p>
3.75)
锛?/p>
for MSC cryopreserved as long as
4 weeks or 9-15 months respectively.However
锛?/p>
there were no changes
detected
锛?/p>
as compared with pre-cryopreserved MSC in immunophenotype
锛?/p>
abilities of proliferation and supporting colony forming of CFU-GM
锛?/p>
CFU-E and CFU-GEMM.It is concluded that bone marrow-derived MSC can
be stored in liquid nitrogen for short-term (4 weeks) or medium-term
(9-15 months) without changes of abilities of proliferation
锛?/p>
differentiation and hematopoiesis support.
Key words
cryopreservation; mesenchymal stem cell
锛?/p>
hematopoiesis support
闂村厖璐ㄥ共缁嗚優锛?/p>
mesenchymal stem cell
锛?/p>
MSC
锛夊叿鏈夊鍚戝垎鍖栧拰鏀寔閫犺鐨勮兘
鍔涳紝鑰屼笖鏉ユ簮骞挎硾锛屽鏄撳湪浣撳鍩瑰吇鍜屾墿澧烇紝鐩墠宸茬粡搴旂敤浜庝复搴婂苟鏄剧ず浜嗚壇
濂界殑娌荤枟鏁堟灉锛?/p>
1-4
锛姐�傚皢鍩瑰吇鍚庣殑
MSC
鍐诲瓨锛屽湪鎮ｈ�呴渶瑕?/p>
MSC
娌荤枟鏃剁粰浜堣緭
娉紝鍏锋湁寰堝ソ鐨勪复搴婂簲鐢ㄥ墠鏅�備絾鏄紝鍐诲瓨鏄惁浼氬奖鍝?/p>
MSC
鐨勭敓鐗╁鐗瑰緛锛屾垜
浠皻涓嶆竻妤氥�傚洜姝わ紝鏈爺绌跺皢楠ㄩ珦鏉ユ簮鐨?/p>
MSC
鍐诲瓨浜?/p>
-
196鈩冩恫姘腑锛岃瀵熶腑鏈?/p>
鍜岀煭鏈熷喕瀛樺悗
MSC
鐨勫娈栥�佸垎鍖栧拰鏀寔閫犺鐨勮兘鍔涘苟鍚屽喕瀛樺墠鐨?/p>
MSC
杩涜姣?/p>
杈冿紝涓鸿繘涓�姝ュ湪涓村簥涓殑搴旂敤鎻愪緵璇﹀敖鐨勫疄楠屼緷鎹�?/p>
鏉愭枡鍜屾柟娉?/p>
璇?/p>
鍓?/p>
IMDM
銆?/p>
DMEM
銆?/p>
DF12
銆?/p>
MCDB
鍩瑰吇娑层�佽儙鐗涜娓呫�侀┈琛�娓呭拰鐢插熀绾ょ淮绱犲潎