IMAGE J
鐏板害鍒嗘瀽
DNA
鐏板害鍒嗘瀽
鏂规硶
1
1.
棣栧厛鎵撳紑杞欢鍚庯紝寮�鍚浘妗ｏ紱
2.
璇峰厛鍋氭牎姝ｏ紝
閫夋嫨
Analyze
搴曚笅鐨?/p>
Calibrate
閫夐」锛?/p>
鍐嶉�夋嫨鏍℃鐨勬ā寮忥紝
浣跨敤
Uncalibrate
OD
锛屽啀鎸?/p>
ok
锛?/p>
3.
鍦?/p>
瑕?/p>
鍒?/p>
鏋?/p>
鐨?/p>
绗?/p>
涓�
鏉?/p>
(first
lane)
鍔?/p>
涓?/p>
涓�
涓?/p>
闀?/p>
鍨?/p>
妗?/p>
(
宸?/p>
鍏?/p>
鍒?/p>
绗?/p>
涓�
涓?/p>
閫?/p>
椤?/p>
)
锛?/p>
鍐?/p>
鎸?/p>
涓?/p>
Analyze/Gels/select first Lane
蹇�熼敭
(Ctr+1)
锛?/p>
姝ゆ椂妗嗘灦涓細鍑虹幇涓�涓彿鐮?/p>
1
锛?/p>
涔嬪悗鍙互绉诲姩妗?/p>
鏋跺埌绗簩涓?/p>
lane
鍐嶉�夋嫨
Analyze/Gels/select second Lane
蹇�熼敭
(Ctr+2)
锛?/p>
褰撶劧鍙互涓�鐩村姞涓嬪幓锛?/p>
鏈�鍚庢寜
Analyze/Gels/plot Lanes
蹇�熼敭
(Ctr +3)
锛?/p>
4.
鍒嗘瀽浠ュ悗浼氬嚭鐜板浘鍨嬭〃绀轰綘鍒氶�夋嫨鐨勬鍐呯殑褰卞儚寮哄害锛?/p>
姝ゆ椂鍙互鐪嬪埌鏈夊嚑涓瘮杈冮珮鐨?/p>
鍖烘锛?/p>
灏辨槸鎴戜滑鎯冲畾閲忕殑
band
锛?/p>
浣跨敤鐩寸嚎宸ュ叿
(
宸ュ叿鍒楃浜斾釜閫夐」
)
鍏堝皢鍥惧舰涓珮鐐逛负鏈?/p>
band
鐨勫尯鍩熷拰娌℃湁
band
鐨勫尯鍩熷垎寮�鍐嶏紝
浣跨敤榄旀湳妫掑伐鍏?/p>
(
宸ュ叿鍒楃鍏釜閫夐」
)
鐐归�夎鍒嗘瀽鐨勫尯鍩燂紱
5.
褰撴垜浠偣閫夊垎鏋愭椂锛屽湪
result
鐨勫璇濊绐椾細鍑虹幇鍒嗘瀽鐨勬暟鎹紝渚濆簭鐐归�夊氨浼氬嚭鐜版瘡
涓?/p>
band
鐨勫�笺�?/p>
鏂规硶
2
1.
鎵撳紑鍥?/p>
2.
閫夋嫨瑕佸垎鏋愮殑鏉″甫锛?/p>
Ctrl+M
娴?/p>
WB
鐏板害鍒嗘瀽
1.
鎵撳紑鏂囦欢锛?/p>
2.
鎶婂浘鐗囪浆鍖栨垚鐏板害鍥剧墖锛?/p>
Image>Type
閫?/p>
8-bit
锛?/p>
3.
娑堥櫎鑳屾櫙褰卞搷锛?/p>
Process>Subtract Background.
閫夋嫨
50
鍩烘湰鍙互銆?/p>
4.
璁剧疆瀹氶噺鍙傛暟锛?/p>
Analyze>Set
Measurements,
鐐瑰嚮闈㈢Н锛屽钩鍧囧瘑搴︼紝鍜岀伆搴﹀�煎強
Integrated Density
锛?/p>
5.
璁剧疆鍗曚綅
Analyze>Set Scale,
鍦ㄢ�?/p>
Unit of length
鈥?/p>
杈圭殑鏂规閲岃緭鍏?/p>
"pixels"
锛?/p>
6.
鎶婂浘鐗囪浆鎹㈡垚浜甫锛?/p>
Edit>Invert
锛?/p>
7.
閫夋嫨
Freehand Selection
锛涘敖閲忔妸鏉″甫鍦堣捣鏉ワ紱
鐐瑰嚮閿洏
m
锛屽嚭鏉?/p>
IntDen
鐏板害鍊硷紱
8.
鐢ㄢ�?/p>
Freehand Selection
鈥濋�夋嫨涓嬩竴鏉″甫锛屾寜
m
杩涜娴嬮噺銆?/p>
11.
褰撴祴瀹氬畬鎵�鏈夋潯甯︼紝閫夌粨鏋滀腑鐨勨�?/p>
Edit
鈥濈殑鈥?/p>
Select
All
鈥?/p>
锛岀劧鍚庡鍒舵暟鎹�?/p>
IntDen
鈥?/p>
鍒?/p>
Excel
琛ㄥ嵆鍙繘琛屽垎鏋愩�?/p>
Merge
1.
鎵撳紑鏂囦欢锛?/p>
2. Image
鈥?/p>
color
鈥?/p>
merge channels
锛?/p>
3.
鍚屾牱鐨勮鎶婅緭鍑虹殑褰╄壊鍥惧儚鍚嶅瓧涓?/p>
composite
绫诲瀷鍙樹负
RGB color Iamge
--
type
--
RGB
color
锛?/p>
4.
鐒跺悗
ctrl+S
淇濆瓨灏卞彲浠ヤ簡銆?/p>