瀹為獙瀹ゅ父鐢ㄦ憾娑茬殑閰嶇疆
Amp
+
/Kan
+
:
璐瓨娑叉祿搴︿负
50mg/mL
绉板彇
500mg
浜?/p>
10mL
瓒呯函姘翠腑婧惰В锛?/p>
鎶芥护鍚?/p>
1mL
鍒嗚鍒扮蹇冪涓紝
浜?/p>
-30
鈩?/p>
鍐诲瓨
X-gal
锛氳串瀛樻祿搴︿负
20mg/mL
绉板彇
20mg X-gal
婧朵簬
1mL
浜岀敳鍩虹敳閰拌兒涓紝
-30
鈩冧腑鐢ㄩ敗绠旂焊鍖呰９鍚庨伩鍏変繚
瀛橈紝涓嶉渶瑕佽繃婊ら櫎鑿?/p>
IPTG
锛氳串瀛樻祿搴︿负
0.84mol/L
绉板彇
2g IPTG
婧朵簬
8mL
瓒呯函姘翠腑锛岀敤姘村畾瀹瑰埌
10mL
锛岀敤
0.22um
鐨勪竴娆℃�?/p>
婊よ繃鍣ㄨ繃婊ら櫎鑿岋紝
1mL
鍒嗚鍒扮蹇冪涓苟浜?/p>
-30
鈩冧繚瀛?/p>
CaCl
2
锛氳串瀛樻祿搴︿负
1mol/L
绉板彇
1.11g
CaCl
锛堟垨鑰?/p>
CaCl:2H
2
O
1.4698g
锛夋憾浜?/p>
8mL
瓒呯函姘翠腑锛屽畾瀹瑰埌
10mL
锛岀敤
0,22um
鐨勪竴娆℃�ф护杩囧櫒杩囨护闄よ弻锛?/p>
1mL
鍒嗚鍒扮蹇冪涓紝骞朵簬
-30
鈩?/p>
淇濆瓨
LB
鍩瑰吇鍩?/p>
锛?/p>
鑳拌泲鐧借儴锛?/p>
Tyrtone
锛?/p>
10g/L
閰垫瘝鎻愬彇鐗╋紙
Yeast Extraction
锛?/p>
5g/L
姘寲閽?/p>
10/L
鏍规嵁缁忛獙鐢?/p>
NaOH
璋冭妭
PH
涓?/p>
7.2-7.6
锛?/p>
鐒跺悗楂樺帇钂告苯鐏弻锛?/p>
娉ㄦ剰鍙婃椂浠庣伃鑿岄攨涓?/p>
鍙栧嚭
鏃?/p>
DNA
閰剁殑
RNA
閰讹細
灏嗚儼
RNA
閰讹紙
RNA
閰?/p>
A
锛夋憾浜?/p>
10mmol/L Tris-Cl
锛?/p>
PH7.5
锛?/p>
銆?/p>
15mmol/L NaCl
涓紝閰嶆垚
10mg/mL
鐨勬祿搴︼紝浜?/p>
100
鈩冨姞鐑?/p>
15min
锛岀紦鎱㈠喎鍗磋嚦瀹ゆ俯锛屽垎瑁呮垚灏?/p>
浠戒繚瀛樹簬
-20
鈩?/p>
Bradford
璐瓨娑?/p>
95%
涔欓唶
100mL
88%
纾烽吀
200mL
G 250
0.35g
瀹ゆ俯涓嬪彲鍌ㄥ瓨锛屼竴鑸?/p>
4
鈩?/p>
Bradford
宸ヤ綔娑?/p>
Bradford
璐瓨娑?/p>
30mL
鍙岃捀姘?/p>
425mL
95%
涔欓唶
15mL
88%
纾烽吀
30mL
鍌ㄥ瓨浜?/p>
4
鈩?/p>