鐩镐俊鑳藉氨涓�瀹氳兘
鏈�鍏ㄦ枃妗ｆ暣鐞?/p>
8
鏂伴檲浠ｈ阿鎬昏涓庣敓鐗╂哀鍖?/p>
1
锛庡凡鐭?/p>
NADH+H
+
缁忓懠鍚搁摼浼犻�掗亣
O
2
鐢熸垚姘寸殑杩囩▼鍙互鐢ㄤ笅寮忚〃绀猴細
NADH + H
+
+ 1/2O
2
H
2
O + NAD
+
璇曡绠楀弽搴旂殑
'
E
飦?/p>
飦?/p>
銆?/p>
'
G
飦?
飦?/p>
銆?/p>
瑙ｇ瓟
锛?/p>
鍦ㄥ懠鍚搁摼涓悇鐢靛瓙瀵规爣鍑嗘哀鍖栬繕鍘熺數浣?/p>
'
E
飦?
鐨勪笉鍚?/p>
,
瀹炶川涓婁篃灏辨槸鑳界骇鐨勪笉鍚屻�傝嚜
鐢辫兘鐨勫彉鍖栧彲浠ョ敱鍙嶅簲鐗╀笌鍙嶅簲浜х墿鐨勬哀鍖栬繕鍘熺數浣嶈绠?/p>
銆?/p>
姘у寲杩樺師鐢典綅鍜岃嚜鐢辫兘鐨勫叧绯诲彲
鐢变互涓嬪叕寮忚绠?/p>
:
'
'
G
nF
E
飦?/p>
飦?/p>
飦?/p>
飥?/p>
飥?/p>
飦?/p>
'
G
飦?/p>
飦?/p>
浠ｈ〃鍙嶅簲鐨勮嚜鐢辫兘
,
n
涓虹數瀛愯浆绉绘暟
,
F
涓?/p>
Farady
甯告暟锛?/p>
鍊间负
96.49kJ/V,
'
E
飦?
飦?/p>
涓?
鐢典綅宸�笺�?/p>
'
G
飦?
飦?/p>
浠?/p>
kJ/mol
璁°�?/p>
NADH+H
+
+
1/2O
2
鈫?/p>
NAD
+
+
H
2
O
G
垄
胃
=-2
脳
96.49
脳
[+0.82 -(-0.32)]
=-
220 kJ/mol
2
锛庡湪鍛煎惛閾句紶閫掔數瀛愮殑绯诲垪姘у寲杩樺師鍙嶅簲涓紝璇锋寚鍑轰笅鍒楀弽搴斾腑鍝簺鏄數瀛愪緵浣擄紝鍝?/p>
浜涙槸鐢靛瓙鍙椾綋锛?/p>
鍝簺鏄哀鍖栧墏锛?/p>
鍝簺鏄繕鍘熷墏
锛?/p>
E-FMN
涓?/p>
NADH
鑴辨阿閰跺鍚堢墿鍚搧纭泲鐧斤紝
杈呭熀涓?/p>
FMN
锛夛紵
锛?/p>
1
锛?/p>
NADH+H
+
+E-FMN
NAD
+
+E-FMNH
2
锛?/p>
2
锛?/p>
E-FMNH
2
+2Fe
3+
E-FMN+2Fe
2+
+2H
+
(3) 2Fe
2+
+2H
+
+Q
2Fe
3+
+QH
2
瑙ｇ瓟
锛?/p>
鍦ㄦ哀鍖栤�曡繕鍘熷弽搴斾腑
,
濡傛灉鍙嶅簲鐗╁け鍘荤數瀛?/p>
,
鍒欒鐗╄川绉颁负杩樺師鍓傦紱
濡傛灉鍙嶅簲鐗╁緱
鍒扮數瀛?/p>
,
鍒欒鍙嶅簲鐗╃О涓烘哀鍖栧墏銆傛墍浠ュ緱鍑哄涓嬬粨璁猴細
鍙嶅簲
鐢靛瓙渚涗綋
鐢靛瓙鍙椾綋
杩樺師鍓?/p>
姘у寲鍓?/p>
锛?/p>
1
锛?/p>
锛?/p>
2
锛?/p>
锛?/p>
3
锛?/p>
NADH
E-FMN
NADH
E-FMN
E-FMNH
2
Fe
3+
E-FMNH
2
Fe
3+
Fe
2+
Q
Fe
2+
Q
3
锛庣粍鎴愬師鐢垫睜鐨勪袱涓崐鐢垫睜锛屽崐鐢垫睜
A
鍚湁
1mol/L
鐨勭敇娌归吀鈥?/p>
3
鈥撶７閰稿拰
1mol/L
鐨?/p>
鐢樻补閱涒�?/p>
3
鈥撶７閰革紝
鑰屽彟澶栫殑涓�涓崐鐢垫睜
B
鍚湁
1mol/L NAD
+
鍜?/p>
1mol/L NADH
銆?/p>
鍥炵瓟涓嬪垪闂?/p>
棰橈細
锛?/p>
1
锛夊摢涓崐鐢垫睜涓彂鐢熺殑鏄哀鍖栧弽搴旓紵
锛?/p>
2
锛夊湪鍗婄數姹?/p>
B
涓紝鍝鐗╄川鐨勬祿搴﹂�愭笎鍑忓皯锛?/p>
(3
锛夌數瀛愭祦鍔ㄧ殑鏂瑰悜濡備綍锛?/p>
锛?/p>
4
锛夋�诲弽搴旓紙
鍗婄數姹?/p>
A+
鍗婄數姹?/p>
B
锛夌殑螖
E
鏄灏戯紵