MEGA
鏋勫缓绯荤粺杩涘寲鏍戠殑姝ラ锛堜互
MEGA7
涓轰緥锛?/p>
鏈枃鏄湅涓浗鎱曡灞变笢澶у鐢熺墿淇℃伅瀛﹁绋嬫�荤粨鍑烘潵鐨?/p>
鍒嗗瓙杩涘寲鐨勭爺绌跺璞℃槸鏍搁吀鍜岃泲鐧借川搴忓垪銆?/p>
鐮旂┒鏌愪釜鍩哄洜鐨勮繘鍖栵紝
鏄敤瀹冪殑
DNA
搴忓垪锛岃繕鏄炕璇戝悗鐨勮泲鐧借川搴忓垪鍛紵搴忓垪鐨勯�夊彇瑕侀伒
寰互涓嬪師鍒欙細
1
锛?/p>
濡傛灉
DNA
搴忓垪鐨勪袱涓ら棿鐨勪竴鑷村害
鈮?0%
锛岄�夌敤
DNA
搴忓垪銆傚洜涓猴紝濡傛灉
DNA
搴忓垪閮藉姝ょ浉浼硷紝瀹冪殑铔嬬櫧璐ㄤ細鐩镐技鍒扮湅涓?/p>
鍑哄尯鍒紝杩欏鏋勫缓绯荤粺鍙戠敓鏍戞槸涓嶅埄鐨勩�傛墍浠ヨ繖绉嶆儏鍐典笅搴旇閫夌敤
DNA
搴忓垪锛岃�屼笉閫夎泲鐧借川搴忓垪銆?/p>
2
锛?/p>
濡傛灉
DNA
搴忓垪鐨勪袱涓ら棿鐨勪竴鑷?/p>
搴?/p>
鈮?0%
锛?/p>
DNA
搴忓垪鍜岃泲鐧借川搴忓垪閮藉彲浠ラ�夌敤銆?/p>
1.
灏嗚鐢ㄤ簬鏋勫缓绯荤粺杩涘寲鏍戠殑鎵�鏈夊簭鍒楀悎骞跺埌鍚屼竴涓?/p>
fasta
鏍煎紡鏂?/p>
浠讹紝娉ㄦ剰锛氭墍鏈夊簭鍒楃殑鏂瑰悜閮借淇濇寔涓�鑷?/p>
( 5
鈥?/p>
-3
鈥?/p>
)
銆?/p>
鎯宠鍋氱郴缁熷彂鐢熸爲鍏堣鍋氬搴忓垪姣斿锛?/p>
鐒跺悗鎶婂搴忓垪姣斿鐨勭粨鏋?/p>
鎻愪氦缁欏缓鏍戣蒋浠惰繘琛屽缓鏍戯紝鎵�浠ュ湪鐢?/p>
MEGA
寤烘爲鏃跺彲浠ヨ緭鍏ヤ竴涓凡
缁忔瘮瀵瑰ソ鐨勫搴忓垪姣斿锛屼篃鍙互杈撳叆涓�鏉″師濮嬪簭鍒楋紝璁?/p>
MEGA
鍏堟潵
鍋氬搴忓垪姣斿锛屽啀寤烘爲锛堜竴鑸垜浠兘鏄師濮嬪簭鍒楋級銆傛墍浠ユ垜浠互鍚庤�?/p>
涓轰緥銆?/p>
2.
鎵撳紑
MEGA
杞欢锛岄�夋嫨涓荤獥鍙ｇ殑鈥?/p>
File
鈥?/p>
鈫?/p>
鈥?/p>
Open A File
鈥濃啋
鎵惧埌骞舵墦寮�
fasta
鏂囦欢锛岃繖鏃朵細璇㈤棶浠ヤ綍绉嶆柟寮忔墦寮�锛屾垜浠槸鍘熷搴?/p>
鍒楋紝闇�瑕佸厛杩涜澶氬簭鍒楁瘮瀵癸紝鎵�浠ラ�夋嫨鈥?/p>
Align
鈥濄�傚鏋滄槸姣斿濂界殑
澶氬簭鍒楁瘮瀵瑰彲浠ョ洿鎺ラ�夋嫨鈥?/p>
Analyze
鈥濄�?/p>
3.
鍦ㄦ墦寮�鐨?/p>
Alignment Explorer
绐楀彛涓�夋嫨鈥?/p>
Alignment
鈥?/p>
-
鈥?/p>
Align by
ClustalW
鈥?/p>
杩涜澶氬簭鍒楁瘮瀵癸紙
MEGA
鎻愪緵浜?/p>
ClustalW
鍜?/p>
Muscle
涓?/p>
绉嶅搴忓垪姣斿鏂规硶锛岃繖閲岄�夋嫨鐔熸倝鐨?/p>
ClustalW
锛夛紝寮瑰嚭绐楀彛璇㈤棶
鈥?/p>
Nothing selected for alignment
锛?/p>
Select all
锛熲�濋�夋嫨鈥?/p>
OK
鈥濄�?/p>
4.
涔嬪悗锛屽脊鍑哄搴忓垪姣斿鍙傛暟璁剧疆绐楀彛銆傝繖涓獥鍙ｅ拰
EMBL
鍦ㄧ嚎
澶氬簭鍒楁瘮瀵逛竴鏍凤紝鍙互璁剧疆鏇挎崲璁板垎鐭╅樀銆佷笉鍚岀殑绌轰綅缃氬垎锛堢綒鍒嗗～
鍐欑殑鏄鏁帮紝璁＄畻鏃舵寜璐熸暟璁＄畻锛夌瓑鍙傛暟銆?/p>
MEGA
鐨勬墍鏈夐粯璁ゅ弬鏁?/p>
閮芥槸缁忚繃鍙嶅鑰冮噺璁剧疆鐨勶紝
杩欎繚璇佷簡
MEGA
鍌荤摐鏈哄叏鑷姩妗ｇ殑鍝佽川锛?/p>
鎵�浠ュ綋浣犳棤浠庝笅鎵嬶紝
鎴栬�呮病鏈変粈涔堢壒鍒姹傜殑鏃跺�欙紝
鐩存帴鐐瑰嚮
鈥?/p>
OK
鈥?/p>
锛?/p>
鎺ュ彈杩欎簺榛樿鍙傛暟锛屽紑濮嬪搴忓垪姣斿銆?/p>