1.
椹搩钖憽钀勭硸鐞艰剛鍩瑰吇鍩?/p>
(PDA
鍩瑰吇鍩?/p>
)
椹搩钖?/p>
(
鍘荤毊
)
200g
钁¤悇绯?/p>
20g
鐞艰剛
20g
姘?/p>
1000mL
pH
鍊?/p>
鑷劧
PDA
鍩瑰吇鍩洪厤鍒舵敞鎰忎簨椤?/p>
1.
鍩瑰吇鍩虹粡鐏弻鍚庯紝蹇呴』鏀惧湪
37C
娓╃鍩瑰吇
24h
锛屾棤鑿岀敓闀胯�呮柟鍙娇鐢ㄣ�?/p>
2.PDA
鍩瑰吇鍩轰竴鑸笉闇�瑕佽皟
pH
銆?/p>
瀵逛簬瑕佽皟鑺?/p>
pH
鐨勫煿鍏诲熀锛?/p>
涓�鑸敤
pH
璇曠焊娴嬪畾鍏?/p>
pH
銆傚鏋滃煿鍏诲熀鍋忛吀鎴栧亸纰辨椂锛屽彲鐢?/p>
lmol/LNaOH
鎴?/p>
lmol/LHCL
婧舵恫杩涜璋冭妭銆?/p>
璋冭妭鏃跺簲閫愭淮鍔犲叆
NaOH
鎴?/p>
HCl
婧舵恫锛岄槻姝㈠眬閮ㄨ繃閰告垨杩囩⒈鐮村潖鍩瑰吇鍩烘垚鍒嗐�?/p>
3.
鍩瑰吇鍩哄湪浣跨敤鏃朵篃鍙互鍋氭垚涓嶅惈鐞艰剛鐨勬恫浣撳煿鍏诲熀锛岀敤浜庤弻绫荤殑闇囪崱鍩瑰吇銆?/p>
4.
鍩瑰吇鍩轰篃鍙互鍔犲叆姘湁绱犳垨鍦熼湁绱狅紝鍔犲叆閲忎负
0.2g/L
鍩瑰吇鍩猴紝涓昏鏄负浜嗘姂
鍒剁粏鑿岀殑鐢熼暱锛屽噺灏戝共鎵版�с�?/p>
15:49:27
澶彞鏃?/p>
2016-4-26
15:49:27
PDA
鍩瑰吇鍩烘槸椹搩钖憽钀勭硸鐞艰剛鍩瑰吇鍩虹殑绠�绉帮紝鍗?
Potato
Dextrose
Agar
(Medium)
锛屽垎鍒搴旈┈閾冭柉銆佽憽钀勭硸銆佺惣鑴傜殑鑻辨枃銆?/p>
瀹冨睘浜庡浐浣撳煿鍏诲熀銆佸崐鍚堟垚鍩瑰吇鍩恒�?/p>
PDA
鍩瑰吇鍩哄疁浜庡井鐢熺墿鐢熼暱鍙戣偛銆佽妭鐪佸師鏂?/p>
绛変紭鍔匡紝涓�鑸湪鍩瑰吇閰垫瘝鑿屻�侀湁鑿屻�佽槕鑿囩瓑鐪熻弻鏂归潰搴旂敤骞挎硾銆?/p>
PDA
鍩瑰吇鍩虹殑閰嶅埗
(1)
绉伴噺鍜岀啲鐓?/p>
鎸夊煿鍏诲熀閰嶆柟閫愪竴绉板彇鍘荤毊鍦熻眴銆傚湡璞嗗垏鎴愬皬鍧楁斁鍏ラ攨涓紝鍔犳按
1000ml
锛屽湪鍔?/p>
鐑櫒涓婂姞鐑嚦娌歌吘锛岀淮鎸?/p>
20-30min
锛岀敤鍙敤
2
灞傜罕甯冭秮鐑湪閲忔澂涓婅繃婊わ紝婊ゆ福
寮冨彇銆傛护娑茶ˉ鍏呮按鍒嗗埌
1000ml
銆?/p>
(2)
鍔犵儹婧惰В
鎶婃护娑叉斁鍏ラ攨涓紝鍔犲叆钁¤悇绯?/p>
20g
锛岀惣鑴?/p>
15
锝?/p>
20g(
鎻愬墠鎼炵
)
锛岀劧鍚庢斁鍦ㄧ煶妫夌綉
涓婏紝灏忕伀鍔犵儹锛屽苟鐢ㄧ幓妫掍笉鏂悈鎷岋紝浠ラ槻鐞艰剛绯婂簳鎴栨孩鍑猴紝寰呯惣鑴傚畬鍏ㄦ憾瑙ｅ悗锛?/p>
鍐嶈ˉ鍏呮按鍒嗚嚦鎵�闇�閲忋�?/p>
(3)
鍒嗚
鎸夊疄璁姹傦紝
灏嗛厤鍒剁殑鍩瑰吇鍩哄垎瑁呭叆璇曠鎴?/p>
500ml
涓夎鐡跺唴銆?/p>
鍒嗚鏃跺彲鐢ㄤ笁瑙掓紡
鏂椾互鍏嶄娇鍩瑰吇鍩烘簿鍦ㄧ鍙ｆ垨鐡跺彛涓婇�犳垚姹℃煋銆?/p>