2011
骞存嫑鏀舵敾璇荤澹浣嶇爺绌剁敓鍏ュ缁熶竴鑰冭瘯璇曢
绉戠洰鍚嶇О锛氱敓鐗╁寲瀛︿笌鍒嗗瓙鐢熺墿瀛?/p>
鎷涚敓鍗曚綅锛氫腑鍥界瀛﹂櫌
涓�锛庡悕璇嶈В閲婏紙姣忛
4
鍒嗭紝鍏?/p>
20
鍒嗭級
锛?/p>
1
锛?/p>
鎶椾綋閰?/p>
锛?/p>
2
锛?/p>
Sanger
鍙岃劚姘х粓姝㈡硶
锛?/p>
3
锛?/p>
DNA
鍒囬櫎淇
锛?/p>
4
锛?/p>
鍏夊悎浣滅敤
锛?/p>
5
锛?/p>
閰垫瘝浜哄伐鏌撹壊浣?/p>
浜岋紟鍗曢�夛紙姣忛
1
鍒嗭紝鍏?/p>
20
鍒嗭級
杩欓噷闈㈢殑棰樺叏鏄熀纭�锛?/p>
姣斿
浜х敓
ATP
鐨勬槸浠�涔堢粏鑳炲櫒锛燂紙绾跨矑浣擄級锛屽悎鎴?/p>
DNA
鐢ㄤ粈涔堝簳鐗╋紵锛?/p>
dNTP
锛変箣绫荤殑锛岄�佸垎鐨勶紝骞舵棤鍙傝�冧环鍊笺�?/p>
涓夛紟鍒ゆ柇锛堟瘡棰?/p>
1
鍒嗭紝鍏?/p>
30
鍒嗭級
杩欎竴閮ㄥ垎鍊煎緱璇翠竴璇达紝
鐭ヨ瘑鐐规湁鐐瑰亸
锛?/p>
浣嗗緢澶氫汉鍖呮嫭浠ュ墠鎴戠殑甯堝厔閮芥病鏈夋彁鍑洪噸
瑙嗭紝鍙兘澶卞垎灏卞湪杩欓噷銆?/p>
鍋氶鎶�宸ц繕鏄湁鐨勶細
棣栧厛
锛屽閿欏悇涓�鍗婏紝杩欐槸鍑洪鍘熷垯銆?/p>
绗簩
锛屽嚭棰樼偣寰堝鏄皬
鏍囬鍐呭銆?/p>
绗笁
锛岄櫡闃辨瘮杈冨锛岃缁嗗績锛岃繖涓笉瑙ｉ噴浣犳噦鐨勩�?/p>
鍥涳紟绠�绛旈锛堟瘡棰?/p>
5
鍒嗭紝鍏?/p>
30
鍒嗭級
1.DNA
鍒嗗瓙鍏嬮殕鐨勬蹇碉紵绠�杩板叾娴佺▼銆?/p>
2.
琛ㄨ閬椾紶瀛︾殑姒傚康锛屽寘鎷摢浜涙柟闈€�傛祬璁〃瑙傞仐浼犲湪鑲跨槫鍙戠敓鍙戝睍杩囩▼涓殑浣?/p>
鐢ㄣ�?/p>
3.
鐪熸牳鐢熺墿涓?/p>
RNA
鑱氬悎閰剁殑绉嶇被浠ュ強涓昏鍔熻兘銆?/p>
4.
绯栬泲鐧戒腑
N-
鍨嬬硸鑲介敭鍜?/p>
O-
鍨嬬硸鑲介敭鐨勭粨鏋勭壒寰佸強浜岃�呯敓鐗╁悎鎴愮殑鐗圭偣鏄粈
涔堬紵
5.
鏌愮爺绌剁敓鍋氬叧浜庢鐗╂柟闈㈢殑瀹為獙锛岄亣鍒颁互涓嬩笁绉嶆儏鍐碉紝璇蜂綘缁欏嚭瀹為獙鏂规硶浠?/p>
鑰岃揪鍒板疄楠岀洰鐨?/p>
锛?/p>
1
锛夋娴嬫煇涓�鐩殑鍩哄洜鏄惁鏁村悎鍒版鐗╃殑鍩哄洜缁勪笂
锛?/p>
2
锛夋娴嬫煇涓�鐩殑鍩哄洜鏄惁杞綍涓?/p>
mRNA