Trizol
娉曚娇鐢ㄦ楠?/p>
涓�銆佸垎绂荤函鍖栫殑鍩烘湰鍘熺悊
鐮旂┒鍩哄洜鐨勮〃杈惧拰璋冩帶鏃跺父甯歌浠庣粍缁囧拰缁嗚優涓垎绂诲拰绾寲
RNA
銆?/p>
RNA
璐ㄩ噺鐨勯珮浣庡父甯稿奖鍝?/p>
cDNA
搴擄紝
RT-PCR
鍜?/p>
Northern
Blot
绛夊垎瀛愮敓鐗╁
瀹為獙鐨勬垚璐ャ�?/p>
Trizol
鏄竴绉嶆柊鍨嬫�?/p>
RNA
鎶芥彁璇曞墏锛屽唴鍚紓纭鞍閰歌儘绛夌墿
璐紝鑳借繀閫熺牬纰庣粏鑳烇紝鎶戝埗缁嗚優閲婃斁鍑虹殑鏍搁吀閰躲�?/p>
浜屻�佺敤鎴烽渶鑷鐨勮瘯鍓傚拰鏉愭枡
鏃犳按涔欓唶銆佹隘浠裤�?/p>
Glycogen
锛堝彲鑳介渶瑕侊級銆?/p>
1.5ml Eppendorf
绠?/p>
锛?/p>
RNase-free
锛夈�?/p>
Tips
锛?/p>
RNase-free
锛?/p>
涓夈�佸噯澶囧伐浣?/p>
RNase
閰堕潪甯哥ǔ瀹氾紝鏄鑷?/p>
RNA
闄嶈В鏈�涓昏鐨勭墿璐ㄣ�傚畠鍦ㄤ竴浜涙瀬绔殑
鏉′欢鍙互鏆傛椂澶辨椿锛屼絾闄愬埗鍥犵礌鍘婚櫎鍚庢湁杩呴�熷鎬с�傜敤甯歌鐨勯珮娓╅珮鍘?/p>
钂告皵鐏弻鏂规硶鍜岃泲鐧芥姂鍒跺墏閮戒笉鑳芥槸
RNase
瀹屽叏澶辨椿銆傚畠骞挎硾瀛樺湪浜庝汉
鐨勭毊鑲や笂锛屽洜姝わ紝鍦ㄤ笌
RNA
鍒跺鏈夊叧鐨勫垎瀛愮敓鐗╁瀹為獙鏃讹紝蹇呴』鎴存墜濂椼�?/p>
RNase
鐨勫張涓�姹℃煋婧愭槸鍙栨恫鍣紝鏍规嵁鍙栨恫鍣ㄥ埗閫犲晢鐨勮姹傚鍙栨恫鍣ㄨ繘琛?/p>
澶勭悊銆?/p>
涓�鑸儏鍐典笅閲囩敤鐢?/p>
DEPC
閰嶅埗鐨?/p>
70%
涔欓唶鎿︽礂鍙栨恫鍣ㄧ殑鍐呴儴鍜屽閮紝
鍩烘湰杈惧埌瑕佹眰銆傚彇
RNase-free
鐨勭墿鍝佹椂蹇呴』鎴存墜濂椼�?/p>
1
銆?/p>
鏂欏埗鍝佺殑澶勭悊
灏藉彲鑳戒娇鐢ㄦ棤鑿岋紝
涓�娆℃�у鏂欏埗鍝侊紝
宸叉爣鏄?/p>
RNase-Free
鐨勫鏂欏埗鍝侊紝
濡傛病鏈夊紑灏佷娇鐢ㄨ繃閫氬父娌℃湁蹇呰鍐嶆澶勭悊銆傚浜庡浗浜у鏂欏埗鍝侊紝鍘熷垯涓?/p>
閮藉繀椤诲鐞嗘柟鍙娇鐢ㄣ�傚鐞嗘楠ゅ涓嬶細
1
锛夊湪鐜荤拑鐑ф澂涓敞鍏ュ幓绂诲瓙姘达紝鍔犲叆
DEPC
浣?/p>
DEPC
鐨勭粓娴撳害涓?/p>
0.1%
銆傛敞
鎰忥細
DEPC
涓哄墽姣掔墿锛屾椿鎬у緢寮猴紝灏忓績鍦ㄩ�氶鏌滀腑浣跨敤銆?/p>
2
锛夊鐞嗙殑濉戞枡鍒跺搧鏀惧叆涓�涓彲浠ラ珮娓╃伃鑿岀殑瀹瑰櫒涓紝娉ㄥ叆
DEPC
姘存憾娑诧紝
浣垮鏂欏埗鍝佺殑鎵�鏈夐儴鍒嗛兘娴告场鍒版憾娑蹭腑銆?/p>
3
锛夊湪閫氶鏌滀腑瀹ゆ俯澶勭悊杩囧銆?/p>
4
锛夊皢
DEPC
姘存憾娑插皬蹇冨�掑埌搴熸恫鐡朵腑锛岀敤閾濈當灏佷綇鍚凡
DEPC
姘村鐞嗚繃鐨?/p>
濉戞枡鍒跺搧鐨勭儳鏉紝楂樻俯楂樺帇钂告皵鐏弻鑷冲皯
30
鍒嗛挓銆?/p>
5
锛夌儤绠辩敤鍚堥�傜殑娓╁害鐑樻嫹鑷冲共鐕ャ�傜疆浜庡共鍑�澶勫鐢ㄣ�?/p>
2
銆?/p>
鐠冪幓鍜岄噾灞炵墿鍝?/p>