1
鎶�鑳借缁?/p>
3
闅鹃澶哄垎鎶�宸ц缁?/p>
1
涓撻璺熻釜璁粌
(
浜屽崄
)
涓�銆佸崟閫夐
1
锛?2018路鍖椾含甯備笢鍩庡尯浜屾ā
)
涓嬪垪鍏充簬鐢熺墿鎶�鏈簲鐢ㄧ殑鍙欒堪锛屾纭殑鏄?/p>
(
)
A
锛庡彲鍒╃敤鍔ㄧ墿浣撶粏鑳炴牳绉绘鎶�鏈埗澶囧崟鍏嬮殕鎶椾綋
B
锛庡彲鍒╃敤妞嶇墿缁勭粐鍩瑰吇鎶�鏈獊鐮寸墿绉嶉棿鐨勭敓娈栭殧绂?/p>
C
锛庡彲鍒╃敤
DNA
閲嶇粍鎶�鏈畾鍚戞敼閫犵敓鐗╃殑閬椾紶鎬х姸
D
锛庡彲鍒╃敤
PCR
鎶�鏈铔嬬櫧璐ㄥ垎瀛愯繘琛屼綋澶栨墿澧?/p>
[
瑙ｆ瀽
]
鍔ㄧ墿缁嗚優宸ョ▼鍖呮嫭鍔ㄧ墿缁嗚優鍩瑰吇銆?/p>
鍔ㄧ墿缁嗚優铻嶅悎绛夛紝
鍙埄鐢ㄥ姩鐗╃粏鑳炶瀺鍚堟妧鏈?/p>
鍒跺鍗曞厠闅嗘姉浣擄紝
A
閿欒锛涙鐗╃粏鑳炲伐绋嬪寘鎷鐗╃粍缁囧煿鍏诲拰妞嶇墿浣撶粏鑳炴潅浜ゆ妧鏈紝鍙埄鐢?/p>
妞嶇墿浣撶粏鑳炴潅浜ゆ妧鏈獊鐮寸墿绉嶉棿鐨勭敓娈栭殧绂伙紝
B
閿欒锛涘熀鍥犲伐绋嬬殑鍘熺悊鏄熀鍥犻噸缁勶紝灞炰簬瀹?/p>
鍚戠殑鐢熺墿鍙樺紓锛屽彲鍒╃敤
DNA
閲嶇粍鎶�鏈畾鍚戞敼閫犵敓鐗╃殑閬椾紶鎬х姸锛?/p>
C
姝ｇ‘锛涘彲鍒╃敤
PCR
鎶�鏈
DNA
鍒嗗瓙杩涜浣撳鎵╁锛屽師鐞嗘槸鍙岄摼
DNA
鍒嗗瓙鐨勫鍒讹紝
D
閿欒銆?/p>
[
绛旀
]
C
2
锛?2018路瀹夊窘鐪佽姕婀栧競妯℃嫙
)CRISPR/Cas9
鍩哄洜缂栬緫鎶�鏈彲瀵瑰熀鍥犺繘琛屽畾鐐圭紪杈戙�傚叾
鍘熺悊鏄敱涓�鏉″崟閾惧悜瀵?/p>
RNA
寮曞鏍搁吀鍐呭垏閰?/p>
Cas9
鍒颁竴涓壒瀹氱殑鍩哄洜浣嶇偣杩涜鍒囧壊銆傞�氳繃璁?/p>
璁″悜瀵?/p>
RNA
涓?/p>
20
涓⒈鍩虹殑璇嗗埆搴忓垪锛屽彲浜轰负閫夋嫨
DNA
涓婄殑鐩爣浣嶇偣杩涜鍒囧壊
(
瑙佷笅鍥?/p>
)
銆備笅
鍒楃浉鍏冲彊杩伴敊璇殑鏄?/p>
(
)
A
锛?/p>
Cas9
铔嬬櫧鑳芥柇瑁傜洰鏍?/p>
DNA
鍒嗗瓙涓殑纾烽吀浜岄叝閿?/p>
B
锛庡崟閾惧悜瀵?/p>
RNA
鍙岄摼鍖轰腑鍢屽懁鏁扮瓑浜庡槯鍟舵暟
C
锛庤杩囩▼鍙互瀵瑰熀鍥犺繘琛屽畾鍚戞敼閫?/p>
D
锛庡崟閾惧悜瀵?/p>
RNA
鍙瘑鍒�佸垏鍓?/p>
DNA
涓婄殑鐩爣浣嶇偣
[
瑙ｆ瀽
]
Cas9
铔嬬櫧涓烘牳閰稿唴鍒囬叾锛?/p>
鑳芥柇瑁傜洰鏍?/p>
DNA
鍒嗗瓙涓殑纾烽吀浜岄叝閿紝
A
姝ｇ‘銆?/p>
鍗曢摼