RNA
骞叉壈鎶�鏈強鍏跺簲鐢?/p>
鏌虫弧
鐢熺墿鍒惰嵂
1201 1202150124
鎽樿锛?/p>
1998
骞?/p>
Andrew
锛?/p>
Fire
鍜?/p>
Craig
C
锛?/p>
Mello
鍙戠幇鍙岄摼
RNA
鍙互鎶戝埗绾?/p>
铏悓婧愬熀鍥犵殑琛ㄨ揪浣垮熀鍥犳矇榛橈紝骞朵笖娌夐粯鍙仐浼犵粰鍚庝唬銆傜幇灏嗚繖绫诲皬骞叉壈
RNA
鍒嗗瓙鍙互楂樻晥銆佺壒寮傚湴闃绘柇浣撳唴鍚屾簮鍩哄洜琛ㄨ揪锛屼績浣垮悓婧?/p>
mRNA
闄嶈В锛岃浣跨粏
鑳炶〃鐜板嚭鍩哄洜缂洪櫡鐨勮〃鍨嬬幇璞＄О涓?/p>
RNA
骞叉壈銆傛湰鏂囦粠
RNAi
鐨勫彂鐜般�佹満鍒躲�佺敓
鐗╁鍔熻兘銆佸簲鐢ㄦ潵璁鸿堪
RNAi
銆傝浆鍩哄洜娌夐粯鐜拌薄鏈�鍒濆湪妞嶇墿涓鍙戠幇銆?/p>
RNAi
鐨?/p>
浣滅敤鏈哄埗锛?/p>
dsRNA
杩涘叆缁嗚優鍐呰
Dicer
閰跺垏鍓叉垚
siRNA
銆?/p>
siRNA
鐨勫弻閾剧粨鏋勮瑙?/p>
铻烘棆涓庢矇榛樺鍚堢墿褰㈡垚澶嶅悎鐗╋紝
siRNA
鍙岄摼琚В寮�锛岀劧鍚庣壒寮傛�х粨鍚堝埌闈?/p>
RNA
涓婏紝鍒囧壊
RNA
銆?/p>
mRNA
鍓垏杩囩▼涓柊褰㈡垚鐨?/p>
21-23
涓?/p>
siRNA
鍙?/p>
鍙互杩涘叆涓嬩竴杞?/p>
寰幆鑰岃揪鍒版墿澧炴晥搴旂殑鐩殑銆傜敓鐗╁鍔熻兘锛氭矇榛樸�佹満浣撳唴婧愭�у熀鍥犺〃杈俱�?/p>
RNAi
鐨勫簲鐢細鍩哄洜鍔熻兘鐨勭爺绌躲�佸熀鍥犲墧闄ゃ�佸尰瀛︽柟闈㈢殑搴旂敤銆?/p>
RNAi
鑽墿銆?/p>
鍏抽敭璇嶏細鍩哄洜娌夐粯
RNAi
siRNA
miRNA
浠ュ墠鎴戝彧鐭ラ亾閬椾紶鐗╄川鏈?/p>
DNA
銆?/p>
RNA
锛屼害鍙煡閬?/p>
RNA
鐨勫姛鑳芥槸杞綍銆備絾
闅忕潃鐭ヨ瘑鐨勫澶氾紝
鎴戜簡瑙ｅ埌鍘熸潵铔嬬櫧璐ㄤ篃鏄竴绉嶉仐浼犵墿璐紝
渚嬪
Prion
铔嬬櫧銆?/p>
Prion
瀛﹁鐨勫嚭鐜颁娇寰楄泲鐧借川涓嶄粎浣撶幇鐢熺墿瀛﹀姛鑳斤紝
鑰屼笖鍙偍瀛橀仐浼犱俊鎭�?/p>
PrPC
铔嬬櫧
楂樼骇缁撴瀯鐨勬敼鍙樹笉浠呭彲閫犳垚铔嬬櫧鍔熻兘鐨勫彉鍖栵紝
鍦ㄦ煇绉嶆剰涔変笂杩樻槸閬椾紶淇℃伅浼犻�掔殑
鏂瑰紡銆傛垜浜︾煡閬撲簡涓嶆槸鎵�鏈夌殑
RNA
閮芥槸鍗曢摼鐨勶紝鏇寸煡閬撲簡
RNA
鐨勫姛鑳戒笉浠呬粎
鍙槸杞綍銆傞�氳繃瀛︿範鍒嗗瓙鐢熺墿瀛︽娴嬫妧鏈紝鎴戠煡閬撲簡
RNA
骞叉壈鐜拌薄鐨勫彂鐜般�佷綔
鐢ㄦ満鍒躲�佸簲鐢ㄧ瓑銆?/p>
1998
骞?/p>
Andrew
锛?/p>
Fire
鍜?/p>
Craig
C
锛?/p>
Mello
鍙戠幇鍙岄摼
RNA
鍙互
鎶戝埗绾胯櫕鍚屾簮鍩哄洜鐨勮〃杈句娇鍩哄洜娌夐粯锛?/p>
骞朵笖娌夐粯鍙仐浼犵粰鍚庝唬銆?/p>
鐜板皢杩欑被灏忓共鎵?/p>
RNA
鍒嗗瓙鍙互楂樻晥銆佺壒寮傚湴闃绘柇浣撳唴鍚屾簮鍩哄洜琛ㄨ揪锛屼績浣垮悓婧?/p>
mRNA
闄嶈В锛岃
浣跨粏鑳炶〃鐜板嚭鍩哄洜缂洪櫡鐨勮〃鍨嬬幇璞＄О涓?/p>
RNA
骞叉壈銆?/p>
2006
骞?/p>
10
鏈?/p>
2
鏃ワ紝鐟炲吀鐨?/p>
瀹跺崱缃楁灄鏂崱
(Karolinska)
鍖诲闄㈠甯冿紝灏?/p>
2006
骞村害璇鸿礉灏旂敓鐞嗗鎴栧尰瀛﹀鎺堜簣
涓ゅ悕缇庡浗绉戝瀹跺畨寰烽瞾路璐归噷
(Andrew
锛?/p>
Fire)
鍜屽厠鎷夋牸路绫虫礇
(Craig C
锛?/p>
Mello)
锛?/p>
浠ヨ〃褰颁粬浠湪
1998
骞寸殑閲嶈鍙戠幇銆傜憺鍏哥殗瀹跺崱缃楁灄鏂崱鍖诲闄㈠湪棰佸澹版槑涓
绉帮紝
璐归噷鍜岀背娲涚殑鍙戠幇鑳借В閲婅澶氫护浜哄洶鎯戙�?/p>
鐩镐簰鐭涚浘鐨勫疄楠岀粨鏋滐紝
骞舵彁绀轰簡鎺?/p>
鍒堕仐浼犱俊鎭祦鍔ㄧ殑鑷劧鏈哄埗銆?/p>
RNAi
鐨勫緢澶氶鍩熻繕鏄湭鐭ョ殑锛岃繖鏈夊緟浜庢垜浠竴浠?/p>
涓�浠ｄ汉鐨勪笉鏂姫鍔涳紝鍘绘彮寮�鍏剁绉樼殑闈㈢罕锛屼负鎴戜滑鑷繁鎻愪緵鏇村ソ鐨勫尰鐤楁潯浠剁瓑銆?/p>