pUC18
鍜?/p>
pUC19
璐ㄧ矑鍥捐氨
pUC18
鍜?/p>
pUC19
澶у皬鍙湁
2686bp
锛屾槸鏈�甯哥敤鐨勮川绮掕浇浣擄紝鍏剁粨鏋勭粍鎴愮揣
鍑戯紝
鍑犱箮涓嶅惈澶氫綑鐨?/p>
DNA
鐗囨锛?/p>
GenBank
娉ㄥ唽鍙蜂负
L08752
锛?/p>
pUC18
锛?/p>
鍜?/p>
X02514
锛?/p>
pUC19
锛?/p>
銆?/p>
鐢?/p>
pBR322
鏀归�犺�屾潵锛?/p>
鍏朵腑
lacZ
锛?/p>
MSC
锛?/p>
鏉ヨ嚜
M13mp18/19
鍥?/p>
3-4
鏄叾璐ㄧ矑鍥捐氨銆?/p>
杩欎袱涓川绮掔殑缁撴瀯鍑犱箮鏄畬鍏ㄤ竴鏍风殑锛?/p>
鍙槸澶氬厠闅嗕綅鐐圭殑鎺掑垪鏂瑰悜鐩稿弽銆?/p>
杩欎簺
璐ㄧ矑缂轰箯鎺у埗鎷疯礉鏁扮殑
rop
鍩哄洜锛屽洜姝ゅ叾鎷疯礉鏁拌揪
500-700
銆?/p>
pUC
绯诲垪杞戒綋
鍚湁涓�娈?/p>
lacZ
铔嬬櫧姘ㄥ熀鏈鐨勯儴鍒嗙紪鐮佸簭鍒楋紝鍦ㄧ壒瀹氱殑鍙椾綋缁嗚優涓彲琛ㄧ幇
伪
-
浜掕ˉ浣滅敤銆傚洜姝ゅ湪澶氬厠闅嗕綅鐐逛腑鎻掑叆浜嗗婧愮墖娈靛悗锛屽彲閫氳繃
伪
-
浜掕ˉ浣滅敤褰㈡垚鐨?/p>
钃濊壊鍜岀櫧鑹茶弻钀界瓫閫夐噸缁勮川绮掋�?/p>
鐗瑰緛搴忓垪鍖轰綅锛?/p>
pBR322_origin
1471 - 852
Ampicillin
2486 - 1626
lac_promoter
543 - 514