TCID
50
娴嬪畾
50
锛呯粍缁囩粏鑳炴劅鏌撻噺
(50
锛?/p>
Tissue culture infection dose, TCID
50
)
鏄?/p>
鎸囪兘浣垮崐鏁板崟灞傜粏鑳炵
(
瀛?/p>
)
鍑虹幇缁嗚優鐥呭彉鐨勭梾姣掔█閲婂害銆傜敤姝ゆ柟娉曞彲浠ヤ及璁?/p>
鐥呮瘨鎰熸煋鎬х殑寮哄急鍙婄梾姣掔殑鍚噺锛屼絾涓嶈兘鍑嗙‘娴嬪畾鎰熸煋鎬х梾姣掗绮掔殑澶氬皯銆?/p>
TCID50
涓?/p>
PFU
鎹㈢畻
:
PFUs
锛?.7脳TCID50
鐨勬淮搴?/p>
锛?/p>
TCID50
娉曟祴鍒扮殑婊村害鍊兼瘮
鏍囧噯绌烘枒娉曢珮
0.7
锛?/p>
PFU
锛氳殌鏂戝舰鎴愬崟浣嶏紝
plaque forming unit
锛夈�傛劅鏌撴�ф淮
搴︾殑鍗曚綅涓�鑸〃绀轰负
PFU/ml
銆?/p>
鐢变簬娴嬪畾
pfu
寰�寰�閲嶅鎬ц緝宸紝
鍥犳杩戜簺骞磋澶?/p>
鐮旂┒鍙堝紑濮嬮噰鐢?/p>
TCID50
鏂规硶鏉ヨ绠楃梾姣掔殑鎰熸煋鍗曚綅銆傚洜姝ゅ缓璁篃鍙娇鐢?/p>
TCID50
娉曘�?/p>
銆愬疄楠屾潗鏂欍�?/p>
1
锛?/p>
宸查暱鎴愬崟灞傜殑
MDCK
缁嗚優鐨?/p>
96
寰瓟鍩瑰吇鏉?/p>
2
锛?/p>
鐢插瀷
H1N1
鐥呮瘨娑?/p>
3
锛?/p>
鐥呮瘨绋�閲婃恫锛堝惈
1.0
碌g/ml
TPCK
鑳伴叾鐨勬棤琛�娓?/p>
DMEM
锛涙敞锛氶櫎浜?/p>
H5
楂樿嚧鐥?/p>
鍨嬫祦鎰熺梾姣掔█閲婃椂鐢ㄦ棤琛�娓?/p>
DMEM
澶栵紝鍏跺畠浜氬瀷鐨勬祦鎰熺梾姣掔█閲婃椂閮界敤鍚?/p>
1.0
碌g/ml
鑳伴叾鐨勬棤琛�娓?/p>
DMEM
銆傛湁鏃?/p>
H5
鍨嬩篃鍔犮�傚姞鍏ヨ儼閰剁殑浣滅敤锛氫娇
HA
瑙ｇ涓哄彂鎸ユ椿鎬х殑褰㈠紡锛?/p>
HA
1
鍜?/p>
HA
0
銆傝繖閲岀敤鐨勮儼閰舵槸瀹為獙瀹や笓闂ㄩ厤鍒剁殑锛?/p>
涓嶅悓浜庝紶缁嗚優鐢ㄧ殑鑳伴叾銆傦級娉細
TPCK
鑳伴叾锛氬師濮嬫祿搴?/p>
100
碌g/ml銆?/p>
4
锛庣伃鑿?/p>
PBS
銆佷簩姘у寲纰筹紙
CO
2
锛夊煿鍏荤銆佸�掔疆鏄惧井闀滅瓑
銆愬疄楠屾楠ゃ�?/p>
1
锛?/p>
鍙栨棤鑿?/p>
EP
绠?/p>
10
鏀紝鍚勭鍒嗗埆鍔犵梾姣掔█閲婃恫
0.9
mL
锛岀劧鍚庡悜绗竴绠″姞鐢?/p>
鍨?/p>
H1N1
鐥呮瘨娑?/p>
0.1mL
锛?/p>
鍙嶅娣峰悎
3
娆★紝
鐢ㄥ彟涓�鏂板惛绠′粠绗竴绠″唴鍚告恫
0.1
mL
鍔犲叆鑷崇浜岀鍐咃紝
鍙嶅娣峰悎
3
娆★紝
鍐嶆崲涓�鏂板惛绠★紝
浠庣浜岀鍚告恫
0.1
mL